Protocol for covalent-targeted and activatable photoacoustic imaging agent for tumor imaging in mice
Summary: Activatable photoacoustic imaging probes offer a strategy to efficiently reduce background noise from endogenous chromophores. We present a protocol for tumor imaging in mice using an activatable covalent photoacoustic imaging probe, NOx-JS013. We describe steps for synthesizing NOx-JS013,...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725004526 |
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| Summary: | Summary: Activatable photoacoustic imaging probes offer a strategy to efficiently reduce background noise from endogenous chromophores. We present a protocol for tumor imaging in mice using an activatable covalent photoacoustic imaging probe, NOx-JS013. We describe steps for synthesizing NOx-JS013, in vitro and in situ validation through gel-based activity-based protein profiling and cellular imaging, and tumor imaging of aggressive prostate cancer mouse models. This approach provides a strategy for mitigating background noise in the development of photoacoustic imaging probes.For complete details on the use and execution of this protocol, please refer to Song et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
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| ISSN: | 2666-1667 |