Au@Pt@HP1-HP2@Fe3O4 Nanoenzymatic Complexes Based on CHA Signal Amplification Strategy for Ultrasensitive SERS Detection of ctDNA in Liver Cancer
Xiaoyong Wang,1,* Jinxin Sheng,1,* Haifan Yang,2,3 Kang Shen,2,3 Jie Yao,1 Yayun Qian,2,3 Gaoyang Chen4 1Department of General Surgery, Nantong Haimen People’s Hospital, Nantong, Jiangsu, People’s Republic of China; 2Institute of Translational Medicine, Medical College, Yangz...
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Dove Medical Press
2025-07-01
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| author | Wang X Sheng J Yang H Shen K Yao J Qian Y Chen G |
| author_facet | Wang X Sheng J Yang H Shen K Yao J Qian Y Chen G |
| author_sort | Wang X |
| collection | DOAJ |
| description | Xiaoyong Wang,1,* Jinxin Sheng,1,* Haifan Yang,2,3 Kang Shen,2,3 Jie Yao,1 Yayun Qian,2,3 Gaoyang Chen4 1Department of General Surgery, Nantong Haimen People’s Hospital, Nantong, Jiangsu, People’s Republic of China; 2Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu, People’s Republic of China; 3The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine, Yangzhou, Jiangsu, People’s Republic of China; 4Department of Oncology, The Affiliated Taizhou Second People’s Hospital of Yangzhou University, Taizhou, Jiangsu, People’s Republic of China*These authors contributed equally to this workCorrespondence: Gaoyang Chen, Department of Oncology, The Affiliated Taizhou Second People’s Hospital of Yangzhou University, Taizhou, Jiangsu, People’s Republic of China, Email taizhouchengaoyang@163.comPurpose: Early diagnosis of liver cancer requires highly sensitive detection of biomarkers. This study aims to develop a novel method for detecting circulating tumor DNA (ctDNA) in the serum of liver cancer patients, leveraging a catalytic hairpin self-assembly (CHA) signal amplification strategy combined with surface-enhanced Raman scattering (SERS) technology and nano-enzyme catalysis.Methods: We synthesized Au@Pt@HP1-HP2@Fe3O4 nano-enzyme complexes, utilizing the SERS-enhancing properties of Pt-coated Au nanoparticles (Au@Pt) and the separation-enrichment capability of Fe3O4 magnetic beads. The complexes catalyzed the oxidation of colorless TMB by H2O2 to produce blue ox-TMB, enabling quantitative detection of PIK3CA E542K mutant ctDNA. The assay’s performance was validated using gold standard qRT-PCR.Results: Under optimized conditions, the method achieved a detection limit for PIK3CA E542K as low as 4.12 aM. The assay demonstrated high sensitivity, specificity, and efficient magnetic separation, making it a robust tool for ctDNA detection.Conclusion: This study presents a highly sensitive and specific detection platform for liver cancer early diagnosis, characterized by magnetic separation and nano-enzyme catalysis. The method holds significant clinical potential for the accurate and early detection of liver cancer biomarkers.Keywords: surface-enhanced Raman scattering, nano-enzymes, circulating tumor DNA, liver cancer, catalytic hairpin self-assembly |
| format | Article |
| id | doaj-art-8bcaf1acbfde4e688f8202084b25954e |
| institution | Kabale University |
| issn | 1178-2013 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Dove Medical Press |
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| series | International Journal of Nanomedicine |
| spelling | doaj-art-8bcaf1acbfde4e688f8202084b25954e2025-08-20T03:28:22ZengDove Medical PressInternational Journal of Nanomedicine1178-20132025-07-01Volume 20Issue 188918905104621Au@Pt@HP1-HP2@Fe3O4 Nanoenzymatic Complexes Based on CHA Signal Amplification Strategy for Ultrasensitive SERS Detection of ctDNA in Liver CancerWang X0Sheng J1Yang H2Shen K3Yao J4Qian Y5Chen G6Department of General SurgeryDepartment of General SurgeryInstitute of Translational Medicine, Medical CollegeInstitute of Translational Medicine, Medical CollegeDepartment of General SurgeryInstitute of Translational Medicine, Medical CollegeDepartment of OncologyXiaoyong Wang,1,* Jinxin Sheng,1,* Haifan Yang,2,3 Kang Shen,2,3 Jie Yao,1 Yayun Qian,2,3 Gaoyang Chen4 1Department of General Surgery, Nantong Haimen People’s Hospital, Nantong, Jiangsu, People’s Republic of China; 2Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu, People’s Republic of China; 3The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine, Yangzhou, Jiangsu, People’s Republic of China; 4Department of Oncology, The Affiliated Taizhou Second People’s Hospital of Yangzhou University, Taizhou, Jiangsu, People’s Republic of China*These authors contributed equally to this workCorrespondence: Gaoyang Chen, Department of Oncology, The Affiliated Taizhou Second People’s Hospital of Yangzhou University, Taizhou, Jiangsu, People’s Republic of China, Email taizhouchengaoyang@163.comPurpose: Early diagnosis of liver cancer requires highly sensitive detection of biomarkers. This study aims to develop a novel method for detecting circulating tumor DNA (ctDNA) in the serum of liver cancer patients, leveraging a catalytic hairpin self-assembly (CHA) signal amplification strategy combined with surface-enhanced Raman scattering (SERS) technology and nano-enzyme catalysis.Methods: We synthesized Au@Pt@HP1-HP2@Fe3O4 nano-enzyme complexes, utilizing the SERS-enhancing properties of Pt-coated Au nanoparticles (Au@Pt) and the separation-enrichment capability of Fe3O4 magnetic beads. The complexes catalyzed the oxidation of colorless TMB by H2O2 to produce blue ox-TMB, enabling quantitative detection of PIK3CA E542K mutant ctDNA. The assay’s performance was validated using gold standard qRT-PCR.Results: Under optimized conditions, the method achieved a detection limit for PIK3CA E542K as low as 4.12 aM. The assay demonstrated high sensitivity, specificity, and efficient magnetic separation, making it a robust tool for ctDNA detection.Conclusion: This study presents a highly sensitive and specific detection platform for liver cancer early diagnosis, characterized by magnetic separation and nano-enzyme catalysis. The method holds significant clinical potential for the accurate and early detection of liver cancer biomarkers.Keywords: surface-enhanced Raman scattering, nano-enzymes, circulating tumor DNA, liver cancer, catalytic hairpin self-assemblyhttps://www.dovepress.com/aupthp1-hp2fe3o4-nanoenzymatic-complexes-based-on-cha-signal-amplifica-peer-reviewed-fulltext-article-IJNSurface-enhanced Raman scatteringnano-enzymescirculating tumor DNAliver cancercatalytic hairpin self-assembly |
| spellingShingle | Wang X Sheng J Yang H Shen K Yao J Qian Y Chen G Au@Pt@HP1-HP2@Fe3O4 Nanoenzymatic Complexes Based on CHA Signal Amplification Strategy for Ultrasensitive SERS Detection of ctDNA in Liver Cancer International Journal of Nanomedicine Surface-enhanced Raman scattering nano-enzymes circulating tumor DNA liver cancer catalytic hairpin self-assembly |
| title | Au@Pt@HP1-HP2@Fe3O4 Nanoenzymatic Complexes Based on CHA Signal Amplification Strategy for Ultrasensitive SERS Detection of ctDNA in Liver Cancer |
| title_full | Au@Pt@HP1-HP2@Fe3O4 Nanoenzymatic Complexes Based on CHA Signal Amplification Strategy for Ultrasensitive SERS Detection of ctDNA in Liver Cancer |
| title_fullStr | Au@Pt@HP1-HP2@Fe3O4 Nanoenzymatic Complexes Based on CHA Signal Amplification Strategy for Ultrasensitive SERS Detection of ctDNA in Liver Cancer |
| title_full_unstemmed | Au@Pt@HP1-HP2@Fe3O4 Nanoenzymatic Complexes Based on CHA Signal Amplification Strategy for Ultrasensitive SERS Detection of ctDNA in Liver Cancer |
| title_short | Au@Pt@HP1-HP2@Fe3O4 Nanoenzymatic Complexes Based on CHA Signal Amplification Strategy for Ultrasensitive SERS Detection of ctDNA in Liver Cancer |
| title_sort | au pt hp1 hp2 fe3o4 nanoenzymatic complexes based on cha signal amplification strategy for ultrasensitive sers detection of ctdna in liver cancer |
| topic | Surface-enhanced Raman scattering nano-enzymes circulating tumor DNA liver cancer catalytic hairpin self-assembly |
| url | https://www.dovepress.com/aupthp1-hp2fe3o4-nanoenzymatic-complexes-based-on-cha-signal-amplifica-peer-reviewed-fulltext-article-IJN |
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