Infectivity of Aflagellar Epimastigotes of Trypanosoma caninum in the DH82 Cell Line and Mouse Peritoneal Macrophages
Background:Trypanosoma caninum presents aflagellar and flagellar epimastigote, trypomastigote, and spheromastigote forms in axenic cultures. Attempts to utilize trypomastigote forms of T. caninum to develop in vitro and in vivo infection models have failed. To investigate the infection potential of...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-01-01
|
| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/japr/7057514 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850212101758386176 |
|---|---|
| author | Kátia Cristina Silva Nascimento Sandra Maria de Oliveira Souza Aline Fagundes Roger Magno Macedo Silva Francisco Odencio Rodrigues de Oliveira Junior Samanta Cristina das Chagas Xavier Gilbert Q. Santos Suzana Corte-Real Juliana Helena da Silva Barros |
| author_facet | Kátia Cristina Silva Nascimento Sandra Maria de Oliveira Souza Aline Fagundes Roger Magno Macedo Silva Francisco Odencio Rodrigues de Oliveira Junior Samanta Cristina das Chagas Xavier Gilbert Q. Santos Suzana Corte-Real Juliana Helena da Silva Barros |
| author_sort | Kátia Cristina Silva Nascimento |
| collection | DOAJ |
| description | Background:Trypanosoma caninum presents aflagellar and flagellar epimastigote, trypomastigote, and spheromastigote forms in axenic cultures. Attempts to utilize trypomastigote forms of T. caninum to develop in vitro and in vivo infection models have failed. To investigate the infection potential of aflagellar epimastigotes, T. caninum interaction studies were performed using DH82 cells and BALB/c mouse peritoneal macrophages in Dulbecco’s modified Eagle medium (DMEM)/F-12 medium supplemented with fetal bovine serum and bovine serum albumin. Light-field microscopy, scanning electron microscopy, and transmission electron microscopy were used to analyze these interactions. Regarding T. caninum–macrophage interactions, the following previously unseen results were obtained: (1) the aflagellar epimastigote form of T. caninum infects macrophages, and (2) T. caninum epimastigotes transformed into amastigotes inside macrophages. Aflagellar epimastigotes were seen adhering to and entering macrophages and differentiating to the amastigote form; amastigotes proliferated within the parasitophorous vacuole in macrophages after 15 min. At the final time point (48 h), there were few macrophages arranged on the coverslips, but interacting with free amastigotes of T. caninum, while some of the parasites changed to the flagellar epimastigote form. Considering the lack of information on T. caninum and its importance in public health, this study provides new insights into the biological cycle of T. caninum and parasite–host relationships. |
| format | Article |
| id | doaj-art-8bc54051d2f04d1c9123dfff0105af34 |
| institution | OA Journals |
| issn | 2090-0031 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-8bc54051d2f04d1c9123dfff0105af342025-08-20T02:09:25ZengWileyJournal of Parasitology Research2090-00312025-01-01202510.1155/japr/7057514Infectivity of Aflagellar Epimastigotes of Trypanosoma caninum in the DH82 Cell Line and Mouse Peritoneal MacrophagesKátia Cristina Silva Nascimento0Sandra Maria de Oliveira Souza1Aline Fagundes2Roger Magno Macedo Silva3Francisco Odencio Rodrigues de Oliveira Junior4Samanta Cristina das Chagas Xavier5Gilbert Q. Santos6Suzana Corte-Real7Juliana Helena da Silva Barros8Trypanosomatid Biology LaboratoryStructural Biology LaboratoryClinical Research and Surveillance in Leishmaniasis LaboratoryRudolf Barth Electron Microscopy PlatformCelular Ultrastructure Laboratory deTrypanosomatid Biology LaboratoryPedagogical Coordination SectionStructural Biology LaboratoryTrypanosomatid Biology LaboratoryBackground:Trypanosoma caninum presents aflagellar and flagellar epimastigote, trypomastigote, and spheromastigote forms in axenic cultures. Attempts to utilize trypomastigote forms of T. caninum to develop in vitro and in vivo infection models have failed. To investigate the infection potential of aflagellar epimastigotes, T. caninum interaction studies were performed using DH82 cells and BALB/c mouse peritoneal macrophages in Dulbecco’s modified Eagle medium (DMEM)/F-12 medium supplemented with fetal bovine serum and bovine serum albumin. Light-field microscopy, scanning electron microscopy, and transmission electron microscopy were used to analyze these interactions. Regarding T. caninum–macrophage interactions, the following previously unseen results were obtained: (1) the aflagellar epimastigote form of T. caninum infects macrophages, and (2) T. caninum epimastigotes transformed into amastigotes inside macrophages. Aflagellar epimastigotes were seen adhering to and entering macrophages and differentiating to the amastigote form; amastigotes proliferated within the parasitophorous vacuole in macrophages after 15 min. At the final time point (48 h), there were few macrophages arranged on the coverslips, but interacting with free amastigotes of T. caninum, while some of the parasites changed to the flagellar epimastigote form. Considering the lack of information on T. caninum and its importance in public health, this study provides new insights into the biological cycle of T. caninum and parasite–host relationships.http://dx.doi.org/10.1155/japr/7057514 |
| spellingShingle | Kátia Cristina Silva Nascimento Sandra Maria de Oliveira Souza Aline Fagundes Roger Magno Macedo Silva Francisco Odencio Rodrigues de Oliveira Junior Samanta Cristina das Chagas Xavier Gilbert Q. Santos Suzana Corte-Real Juliana Helena da Silva Barros Infectivity of Aflagellar Epimastigotes of Trypanosoma caninum in the DH82 Cell Line and Mouse Peritoneal Macrophages Journal of Parasitology Research |
| title | Infectivity of Aflagellar Epimastigotes of Trypanosoma caninum in the DH82 Cell Line and Mouse Peritoneal Macrophages |
| title_full | Infectivity of Aflagellar Epimastigotes of Trypanosoma caninum in the DH82 Cell Line and Mouse Peritoneal Macrophages |
| title_fullStr | Infectivity of Aflagellar Epimastigotes of Trypanosoma caninum in the DH82 Cell Line and Mouse Peritoneal Macrophages |
| title_full_unstemmed | Infectivity of Aflagellar Epimastigotes of Trypanosoma caninum in the DH82 Cell Line and Mouse Peritoneal Macrophages |
| title_short | Infectivity of Aflagellar Epimastigotes of Trypanosoma caninum in the DH82 Cell Line and Mouse Peritoneal Macrophages |
| title_sort | infectivity of aflagellar epimastigotes of trypanosoma caninum in the dh82 cell line and mouse peritoneal macrophages |
| url | http://dx.doi.org/10.1155/japr/7057514 |
| work_keys_str_mv | AT katiacristinasilvanascimento infectivityofaflagellarepimastigotesoftrypanosomacaninuminthedh82celllineandmouseperitonealmacrophages AT sandramariadeoliveirasouza infectivityofaflagellarepimastigotesoftrypanosomacaninuminthedh82celllineandmouseperitonealmacrophages AT alinefagundes infectivityofaflagellarepimastigotesoftrypanosomacaninuminthedh82celllineandmouseperitonealmacrophages AT rogermagnomacedosilva infectivityofaflagellarepimastigotesoftrypanosomacaninuminthedh82celllineandmouseperitonealmacrophages AT franciscoodenciorodriguesdeoliveirajunior infectivityofaflagellarepimastigotesoftrypanosomacaninuminthedh82celllineandmouseperitonealmacrophages AT samantacristinadaschagasxavier infectivityofaflagellarepimastigotesoftrypanosomacaninuminthedh82celllineandmouseperitonealmacrophages AT gilbertqsantos infectivityofaflagellarepimastigotesoftrypanosomacaninuminthedh82celllineandmouseperitonealmacrophages AT suzanacortereal infectivityofaflagellarepimastigotesoftrypanosomacaninuminthedh82celllineandmouseperitonealmacrophages AT julianahelenadasilvabarros infectivityofaflagellarepimastigotesoftrypanosomacaninuminthedh82celllineandmouseperitonealmacrophages |