Antibacterial Effects of Er:YAG Laser Irradiation on <i>Candida–Streptococcal</i> Biofilms
In contemporary dentistry, laser-based interventions have become one of the mainstays of care for patients with oral biofilm diseases, such as candidiasis, periodontal disease and peri-implantitis. The purpose of this study was to evaluate the effectiveness of Er:YAG laser (LightWalker, Ljubljana, F...
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2025-03-01
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| author | Zuzanna Grzech-Leśniak Jakub Pyrkosz Jagoda Szwach Patrycja Kosidło Jacek Matys Rafał Wiench Magdalena Pajączkowska Joanna Nowicka Marzena Dominiak Kinga Grzech-Leśniak |
| author_facet | Zuzanna Grzech-Leśniak Jakub Pyrkosz Jagoda Szwach Patrycja Kosidło Jacek Matys Rafał Wiench Magdalena Pajączkowska Joanna Nowicka Marzena Dominiak Kinga Grzech-Leśniak |
| author_sort | Zuzanna Grzech-Leśniak |
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| description | In contemporary dentistry, laser-based interventions have become one of the mainstays of care for patients with oral biofilm diseases, such as candidiasis, periodontal disease and peri-implantitis. The purpose of this study was to evaluate the effectiveness of Er:YAG laser (LightWalker, Ljubljana, Fotona, Slovenia) irradiation at varying irradiance levels (T1: 11.3 W/cm<sup>2</sup> and T2: 120.54 W/cm<sup>2</sup>) on microbial viability in single- and dual-species biofilm models, focusing on <i>Candida albicans</i>, <i>Candida glabrata</i> and <i>Streptococcus mutans</i>, to address challenges in managing complex oral biofilms in clinically relevant settings. The results showed substantial microbial reduction, with <i>C. albicans</i> being the most susceptible microorganism (93–99.9%), while <i>C. glabrata</i> exhibited marked resistance at higher irradiance levels. Interestingly, <i>S. mutans</i> demonstrated varying reductions based on the biofilm composition, highlighting the influence of microbial interactions. This study concluded that the Er:YAG laser effectively reduced biofilm viability, with its efficacy depending on the microbial composition and irradiance settings. These findings highlight the need for tailored erbium laser parameters to optimize clinical outcomes, underscoring the need for individualized polymicrobial biofilm management, particularly in periodontal and peri-implant therapies. |
| format | Article |
| id | doaj-art-8bc2fac3735c4bbfb9efd49d78c205d5 |
| institution | OA Journals |
| issn | 2075-1729 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj-art-8bc2fac3735c4bbfb9efd49d78c205d52025-08-20T02:11:24ZengMDPI AGLife2075-17292025-03-0115347410.3390/life15030474Antibacterial Effects of Er:YAG Laser Irradiation on <i>Candida–Streptococcal</i> BiofilmsZuzanna Grzech-Leśniak0Jakub Pyrkosz1Jagoda Szwach2Patrycja Kosidło3Jacek Matys4Rafał Wiench5Magdalena Pajączkowska6Joanna Nowicka7Marzena Dominiak8Kinga Grzech-Leśniak9Faculty of Medicine and Dentistry, Wroclaw Medical University, 50-367 Wroclaw, PolandFaculty of Medicine, Wroclaw Medical University, 50-367 Wroclaw, PolandFaculty of Medicine, Wroclaw Medical University, 50-367 Wroclaw, PolandFaculty of Medicine, Wroclaw Medical University, 50-367 Wroclaw, PolandLaser Laboratory, Dental Surgery Department, Faculty of Dentistry, Wroclaw Medical University, 50-367 Wroclaw, PolandDepartment of Periodontal Diseases and Oral Mucosa Diseases, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-055 Katowice, PolandDepartment of Microbiology, Faculty of Medicine, Wroclaw Medical University, 50-367 Wroclaw, PolandDepartment of Microbiology, Faculty of Medicine, Wroclaw Medical University, 50-367 Wroclaw, PolandLaser Laboratory, Dental Surgery Department, Faculty of Dentistry, Wroclaw Medical University, 50-367 Wroclaw, PolandLaser Laboratory, Dental Surgery Department, Faculty of Dentistry, Wroclaw Medical University, 50-367 Wroclaw, PolandIn contemporary dentistry, laser-based interventions have become one of the mainstays of care for patients with oral biofilm diseases, such as candidiasis, periodontal disease and peri-implantitis. The purpose of this study was to evaluate the effectiveness of Er:YAG laser (LightWalker, Ljubljana, Fotona, Slovenia) irradiation at varying irradiance levels (T1: 11.3 W/cm<sup>2</sup> and T2: 120.54 W/cm<sup>2</sup>) on microbial viability in single- and dual-species biofilm models, focusing on <i>Candida albicans</i>, <i>Candida glabrata</i> and <i>Streptococcus mutans</i>, to address challenges in managing complex oral biofilms in clinically relevant settings. The results showed substantial microbial reduction, with <i>C. albicans</i> being the most susceptible microorganism (93–99.9%), while <i>C. glabrata</i> exhibited marked resistance at higher irradiance levels. Interestingly, <i>S. mutans</i> demonstrated varying reductions based on the biofilm composition, highlighting the influence of microbial interactions. This study concluded that the Er:YAG laser effectively reduced biofilm viability, with its efficacy depending on the microbial composition and irradiance settings. These findings highlight the need for tailored erbium laser parameters to optimize clinical outcomes, underscoring the need for individualized polymicrobial biofilm management, particularly in periodontal and peri-implant therapies.https://www.mdpi.com/2075-1729/15/3/474<i>Candida albicans</i><i>Candida glabrata</i>Erbium laser<i>Streptococcus mutans</i> |
| spellingShingle | Zuzanna Grzech-Leśniak Jakub Pyrkosz Jagoda Szwach Patrycja Kosidło Jacek Matys Rafał Wiench Magdalena Pajączkowska Joanna Nowicka Marzena Dominiak Kinga Grzech-Leśniak Antibacterial Effects of Er:YAG Laser Irradiation on <i>Candida–Streptococcal</i> Biofilms Life <i>Candida albicans</i> <i>Candida glabrata</i> Erbium laser <i>Streptococcus mutans</i> |
| title | Antibacterial Effects of Er:YAG Laser Irradiation on <i>Candida–Streptococcal</i> Biofilms |
| title_full | Antibacterial Effects of Er:YAG Laser Irradiation on <i>Candida–Streptococcal</i> Biofilms |
| title_fullStr | Antibacterial Effects of Er:YAG Laser Irradiation on <i>Candida–Streptococcal</i> Biofilms |
| title_full_unstemmed | Antibacterial Effects of Er:YAG Laser Irradiation on <i>Candida–Streptococcal</i> Biofilms |
| title_short | Antibacterial Effects of Er:YAG Laser Irradiation on <i>Candida–Streptococcal</i> Biofilms |
| title_sort | antibacterial effects of er yag laser irradiation on i candida streptococcal i biofilms |
| topic | <i>Candida albicans</i> <i>Candida glabrata</i> Erbium laser <i>Streptococcus mutans</i> |
| url | https://www.mdpi.com/2075-1729/15/3/474 |
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