Ammonium based dicationic ionic liquids: An interaction with CRABP I deciphered with biophysical perspective
The present article examined the impact of ammonium-based dicationic ionic liquids (ILs) as co-solvent on the biophysical behaviour of CRABP I protein using various spectroscopic techniques along with molecular docking to support the experimental outcomes. Fluorescence study revealed significant alt...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Journal of Ionic Liquids |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772422025000199 |
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| author | Pratyush Ranjan Hota Devi Prasanna Behera Harekrushna Sahoo |
| author_facet | Pratyush Ranjan Hota Devi Prasanna Behera Harekrushna Sahoo |
| author_sort | Pratyush Ranjan Hota |
| collection | DOAJ |
| description | The present article examined the impact of ammonium-based dicationic ionic liquids (ILs) as co-solvent on the biophysical behaviour of CRABP I protein using various spectroscopic techniques along with molecular docking to support the experimental outcomes. Fluorescence study revealed significant alternations in the microenvironments of aromatic fluorescent amino acid residues. Fluorescence intensity indicated a decline upon increase in concentration of co-solvents, with a red shift. A similar trend also observed with the synchronous study. Stern-Volmer parameters revealed the static quenching behaviour whereas, existence of electrostatic interaction between protein and ILs is revealed from binding study using temperature-dependent fluorescence measurements. Conformational and structural changes of the protein were analysed using Circular Dichroism spectrometer. Molecular docking study supported the spectroscopic outcomes at the molecular level. |
| format | Article |
| id | doaj-art-8bc003dab0c44a5da2987aa36f7dd4c9 |
| institution | DOAJ |
| issn | 2772-4220 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ionic Liquids |
| spelling | doaj-art-8bc003dab0c44a5da2987aa36f7dd4c92025-08-20T03:10:25ZengElsevierJournal of Ionic Liquids2772-42202025-06-015110015010.1016/j.jil.2025.100150Ammonium based dicationic ionic liquids: An interaction with CRABP I deciphered with biophysical perspectivePratyush Ranjan Hota0Devi Prasanna Behera1Harekrushna Sahoo2Biophysical and Protein Chemistry Lab, Department of Chemistry, National Institute of Technology, Rourkela 769008, Odisha, IndiaBiophysical and Protein Chemistry Lab, Department of Chemistry, National Institute of Technology, Rourkela 769008, Odisha, IndiaBiophysical and Protein Chemistry Lab, Department of Chemistry, National Institute of Technology, Rourkela 769008, Odisha, India; Center for Nanomaterials, National Institute of Technology, Rourkela 769008, Odisha, India; Corresponding author.The present article examined the impact of ammonium-based dicationic ionic liquids (ILs) as co-solvent on the biophysical behaviour of CRABP I protein using various spectroscopic techniques along with molecular docking to support the experimental outcomes. Fluorescence study revealed significant alternations in the microenvironments of aromatic fluorescent amino acid residues. Fluorescence intensity indicated a decline upon increase in concentration of co-solvents, with a red shift. A similar trend also observed with the synchronous study. Stern-Volmer parameters revealed the static quenching behaviour whereas, existence of electrostatic interaction between protein and ILs is revealed from binding study using temperature-dependent fluorescence measurements. Conformational and structural changes of the protein were analysed using Circular Dichroism spectrometer. Molecular docking study supported the spectroscopic outcomes at the molecular level.http://www.sciencedirect.com/science/article/pii/S2772422025000199CRABP IAmmonium based dicationic ionic liquidsFluorescenceCircular dichroismMolecular docking |
| spellingShingle | Pratyush Ranjan Hota Devi Prasanna Behera Harekrushna Sahoo Ammonium based dicationic ionic liquids: An interaction with CRABP I deciphered with biophysical perspective Journal of Ionic Liquids CRABP I Ammonium based dicationic ionic liquids Fluorescence Circular dichroism Molecular docking |
| title | Ammonium based dicationic ionic liquids: An interaction with CRABP I deciphered with biophysical perspective |
| title_full | Ammonium based dicationic ionic liquids: An interaction with CRABP I deciphered with biophysical perspective |
| title_fullStr | Ammonium based dicationic ionic liquids: An interaction with CRABP I deciphered with biophysical perspective |
| title_full_unstemmed | Ammonium based dicationic ionic liquids: An interaction with CRABP I deciphered with biophysical perspective |
| title_short | Ammonium based dicationic ionic liquids: An interaction with CRABP I deciphered with biophysical perspective |
| title_sort | ammonium based dicationic ionic liquids an interaction with crabp i deciphered with biophysical perspective |
| topic | CRABP I Ammonium based dicationic ionic liquids Fluorescence Circular dichroism Molecular docking |
| url | http://www.sciencedirect.com/science/article/pii/S2772422025000199 |
| work_keys_str_mv | AT pratyushranjanhota ammoniumbaseddicationicionicliquidsaninteractionwithcrabpidecipheredwithbiophysicalperspective AT deviprasannabehera ammoniumbaseddicationicionicliquidsaninteractionwithcrabpidecipheredwithbiophysicalperspective AT harekrushnasahoo ammoniumbaseddicationicionicliquidsaninteractionwithcrabpidecipheredwithbiophysicalperspective |