The in ovo screening of 27 single essential oils showed selective effects on hatchability, performance and gene expression relevant to gut functions in broilers at hatch
The early post-hatching phase remains to be one of the most vulnerable phases in broiler production. Some essential oils have been reported to improve gut health and growth in broiler chickens when applied to post-hatching diets. However, in-feed applications are unable to prevent the health challen...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124012483 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832591853612957696 |
|---|---|
| author | Asad A. Khaskheli Shahram Niknafs Mila M.Y. Meijer Xinle Tan Peter R. Ferket Eugeni Roura |
| author_facet | Asad A. Khaskheli Shahram Niknafs Mila M.Y. Meijer Xinle Tan Peter R. Ferket Eugeni Roura |
| author_sort | Asad A. Khaskheli |
| collection | DOAJ |
| description | The early post-hatching phase remains to be one of the most vulnerable phases in broiler production. Some essential oils have been reported to improve gut health and growth in broiler chickens when applied to post-hatching diets. However, in-feed applications are unable to prevent the health challenges observed immediately after hatching. Thus, pre-hatch interventions need to be considered. A research project was developed with the aim of investigating the impact of in ovo application of 27 selected essential oils (EOs) on foetal development with emphasis on gut integrity in broiler hatchlings. The eggs were incubated under standard conditions until day 17.5, when 1 mL of each EO preparation (5 µL EO + 5 µL polysorbate-80 + 990 µL saline) was injected into the amnion. Hatchability, body weight and organ weights (residual yolk, gizzard-proventriculus, intestines, liver, and heart) were measured at hatch. Five essential oils eugenol, clove, tea tree, lemongrass, and thyme, significantly (P < 0.05) reduced hatchability (66.67 %, 58.33, 83.30 and 83.30 %) compared to the saline (96.80 %), were discarded from the rest of the study. The other 22 essential oils were investigated in a second phase to assess their impact on expression of gut biomarkers including: a) jejunum integrity; b) digestive enzymes and nutrient transporters; and c) immune system. The results indicated that lemon myrtle significantly increased and oregano EO decreased body weight at hatch (BW0) compared to the saline (P < 0.05). Ylang ylang, clary sage, bergamot, lemon myrtle, and black pepper upregulated the expressions of biomarkers regulating gut integrity and barrier functions (ZO-1, ZO-2, CLDN1, MARVELD2, EGFR and EGF), nutrients transporters (EAAT3, PEPT1, I-FABP1, SGLT1), and digestive enzymes (APN, SI). Ylang ylang, turmeric acid, star anise, clary sage, and black pepper upregulated the expression of gut immunity biomarkers IL1B, IL10, IGMH, CD3D, and BU1 compared to the saline. In conclusion, in ovo delivery of selected EOs has the potential to improve embryonic development relevant to nutrient digestion and absorption, gut integrity and immunity in broilers. |
| format | Article |
| id | doaj-art-8bbff5c73dcd40bc910150a8b50f12b1 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-8bbff5c73dcd40bc910150a8b50f12b12025-01-22T05:40:53ZengElsevierPoultry Science0032-57912025-01-011041104670The in ovo screening of 27 single essential oils showed selective effects on hatchability, performance and gene expression relevant to gut functions in broilers at hatchAsad A. Khaskheli0Shahram Niknafs1Mila M.Y. Meijer2Xinle Tan3Peter R. Ferket4Eugeni Roura5Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, AustraliaQueensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, AustraliaQueensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, AustraliaQueensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, AustraliaPrestage Department of Poultry Science, North Carolina State University, Raleigh, USAQueensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Australia; Corresponding author: Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, Australia.The early post-hatching phase remains to be one of the most vulnerable phases in broiler production. Some essential oils have been reported to improve gut health and growth in broiler chickens when applied to post-hatching diets. However, in-feed applications are unable to prevent the health challenges observed immediately after hatching. Thus, pre-hatch interventions need to be considered. A research project was developed with the aim of investigating the impact of in ovo application of 27 selected essential oils (EOs) on foetal development with emphasis on gut integrity in broiler hatchlings. The eggs were incubated under standard conditions until day 17.5, when 1 mL of each EO preparation (5 µL EO + 5 µL polysorbate-80 + 990 µL saline) was injected into the amnion. Hatchability, body weight and organ weights (residual yolk, gizzard-proventriculus, intestines, liver, and heart) were measured at hatch. Five essential oils eugenol, clove, tea tree, lemongrass, and thyme, significantly (P < 0.05) reduced hatchability (66.67 %, 58.33, 83.30 and 83.30 %) compared to the saline (96.80 %), were discarded from the rest of the study. The other 22 essential oils were investigated in a second phase to assess their impact on expression of gut biomarkers including: a) jejunum integrity; b) digestive enzymes and nutrient transporters; and c) immune system. The results indicated that lemon myrtle significantly increased and oregano EO decreased body weight at hatch (BW0) compared to the saline (P < 0.05). Ylang ylang, clary sage, bergamot, lemon myrtle, and black pepper upregulated the expressions of biomarkers regulating gut integrity and barrier functions (ZO-1, ZO-2, CLDN1, MARVELD2, EGFR and EGF), nutrients transporters (EAAT3, PEPT1, I-FABP1, SGLT1), and digestive enzymes (APN, SI). Ylang ylang, turmeric acid, star anise, clary sage, and black pepper upregulated the expression of gut immunity biomarkers IL1B, IL10, IGMH, CD3D, and BU1 compared to the saline. In conclusion, in ovo delivery of selected EOs has the potential to improve embryonic development relevant to nutrient digestion and absorption, gut integrity and immunity in broilers.http://www.sciencedirect.com/science/article/pii/S0032579124012483Essential oilsin ovoGut integrityDigestive enzymesImmune system |
| spellingShingle | Asad A. Khaskheli Shahram Niknafs Mila M.Y. Meijer Xinle Tan Peter R. Ferket Eugeni Roura The in ovo screening of 27 single essential oils showed selective effects on hatchability, performance and gene expression relevant to gut functions in broilers at hatch Poultry Science Essential oils in ovo Gut integrity Digestive enzymes Immune system |
| title | The in ovo screening of 27 single essential oils showed selective effects on hatchability, performance and gene expression relevant to gut functions in broilers at hatch |
| title_full | The in ovo screening of 27 single essential oils showed selective effects on hatchability, performance and gene expression relevant to gut functions in broilers at hatch |
| title_fullStr | The in ovo screening of 27 single essential oils showed selective effects on hatchability, performance and gene expression relevant to gut functions in broilers at hatch |
| title_full_unstemmed | The in ovo screening of 27 single essential oils showed selective effects on hatchability, performance and gene expression relevant to gut functions in broilers at hatch |
| title_short | The in ovo screening of 27 single essential oils showed selective effects on hatchability, performance and gene expression relevant to gut functions in broilers at hatch |
| title_sort | in ovo screening of 27 single essential oils showed selective effects on hatchability performance and gene expression relevant to gut functions in broilers at hatch |
| topic | Essential oils in ovo Gut integrity Digestive enzymes Immune system |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124012483 |
| work_keys_str_mv | AT asadakhaskheli theinovoscreeningof27singleessentialoilsshowedselectiveeffectsonhatchabilityperformanceandgeneexpressionrelevanttogutfunctionsinbroilersathatch AT shahramniknafs theinovoscreeningof27singleessentialoilsshowedselectiveeffectsonhatchabilityperformanceandgeneexpressionrelevanttogutfunctionsinbroilersathatch AT milamymeijer theinovoscreeningof27singleessentialoilsshowedselectiveeffectsonhatchabilityperformanceandgeneexpressionrelevanttogutfunctionsinbroilersathatch AT xinletan theinovoscreeningof27singleessentialoilsshowedselectiveeffectsonhatchabilityperformanceandgeneexpressionrelevanttogutfunctionsinbroilersathatch AT peterrferket theinovoscreeningof27singleessentialoilsshowedselectiveeffectsonhatchabilityperformanceandgeneexpressionrelevanttogutfunctionsinbroilersathatch AT eugeniroura theinovoscreeningof27singleessentialoilsshowedselectiveeffectsonhatchabilityperformanceandgeneexpressionrelevanttogutfunctionsinbroilersathatch AT asadakhaskheli inovoscreeningof27singleessentialoilsshowedselectiveeffectsonhatchabilityperformanceandgeneexpressionrelevanttogutfunctionsinbroilersathatch AT shahramniknafs inovoscreeningof27singleessentialoilsshowedselectiveeffectsonhatchabilityperformanceandgeneexpressionrelevanttogutfunctionsinbroilersathatch AT milamymeijer inovoscreeningof27singleessentialoilsshowedselectiveeffectsonhatchabilityperformanceandgeneexpressionrelevanttogutfunctionsinbroilersathatch AT xinletan inovoscreeningof27singleessentialoilsshowedselectiveeffectsonhatchabilityperformanceandgeneexpressionrelevanttogutfunctionsinbroilersathatch AT peterrferket inovoscreeningof27singleessentialoilsshowedselectiveeffectsonhatchabilityperformanceandgeneexpressionrelevanttogutfunctionsinbroilersathatch AT eugeniroura inovoscreeningof27singleessentialoilsshowedselectiveeffectsonhatchabilityperformanceandgeneexpressionrelevanttogutfunctionsinbroilersathatch |