Phenolic compounds from Chaenomeles speciosa alleviate inflammation in lipopolysaccharide-treated RAW264.7 macrophages via the NF-κB and MAPK pathways
Chaenomeles speciosa (Sweet) Nakai cultivated widely in temperate regions possesses anti-inflammatory properties, however, the underlying molecular mechanisms remain not fully understood. In this study, a purified phenolic extract of C. speciosa rich in chlorogenic acid, procyanidin B1 and catechin...
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| Format: | Article |
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Tsinghua University Press
2023-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002440 |
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| author | Fuxia Hu Chao Liu Fengqin Wang Changxin Zhou Maotong Zhu Dongxiao Sun-Waterhouse Zhaosheng Wang |
| author_facet | Fuxia Hu Chao Liu Fengqin Wang Changxin Zhou Maotong Zhu Dongxiao Sun-Waterhouse Zhaosheng Wang |
| author_sort | Fuxia Hu |
| collection | DOAJ |
| description | Chaenomeles speciosa (Sweet) Nakai cultivated widely in temperate regions possesses anti-inflammatory properties, however, the underlying molecular mechanisms remain not fully understood. In this study, a purified phenolic extract of C. speciosa rich in chlorogenic acid, procyanidin B1 and catechin (determined by HPLC-Q-TOF-MS/MS) exhibited dose-dependent anti-inflammatory effects on lipopolysaccharide (LPS)-treated RAW264.7 macrophages. The extract at 30 μg/mL was most potent and enabled most cells in normal morphology under LPS stimulation without causing cytotoxicity. The extract suppressed the levels of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β), and the mRNA and protein expressions of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). The mechanisms underlying such anti-inflammatory actions included the regulation of phosphorylation of related proteins to monitor the expressions of inflammatory mediators and genes in the nuclear factor kappa-B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways. Therefore, the phenolic extract from C. speciosa is a desirable anti-inflammatory agent for inflammatory conditions to meet the rising demand for natural and cost-effective therapeutics. |
| format | Article |
| id | doaj-art-8bbb0a2299884108b916d1cdcf002d6a |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-8bbb0a2299884108b916d1cdcf002d6a2025-02-03T11:09:30ZengTsinghua University PressFood Science and Human Wellness2213-45302023-07-0112410711080Phenolic compounds from Chaenomeles speciosa alleviate inflammation in lipopolysaccharide-treated RAW264.7 macrophages via the NF-κB and MAPK pathwaysFuxia Hu0Chao Liu1Fengqin Wang2Changxin Zhou3Maotong Zhu4Dongxiao Sun-Waterhouse5Zhaosheng Wang6Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, ChinaKey Laboratory of Novel Food Resources Processing, Ministry of Agriculture and Rural Affairs, Key Laboratory of Agro-Products Processing Technology of Shandong Province, Institute of Agro-Food Science and Technology, Jinan 250100, ChinaKey Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, ChinaKey Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, ChinaKey Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, ChinaSchool of Chemical Sciences, The University of Auckland, Private Bag 92019, New Zealand; Corresponding authors.Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China; Corresponding authors.Chaenomeles speciosa (Sweet) Nakai cultivated widely in temperate regions possesses anti-inflammatory properties, however, the underlying molecular mechanisms remain not fully understood. In this study, a purified phenolic extract of C. speciosa rich in chlorogenic acid, procyanidin B1 and catechin (determined by HPLC-Q-TOF-MS/MS) exhibited dose-dependent anti-inflammatory effects on lipopolysaccharide (LPS)-treated RAW264.7 macrophages. The extract at 30 μg/mL was most potent and enabled most cells in normal morphology under LPS stimulation without causing cytotoxicity. The extract suppressed the levels of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β), and the mRNA and protein expressions of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). The mechanisms underlying such anti-inflammatory actions included the regulation of phosphorylation of related proteins to monitor the expressions of inflammatory mediators and genes in the nuclear factor kappa-B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways. Therefore, the phenolic extract from C. speciosa is a desirable anti-inflammatory agent for inflammatory conditions to meet the rising demand for natural and cost-effective therapeutics.http://www.sciencedirect.com/science/article/pii/S2213453022002440Chaenomeles speciosaPhenolic extractPurificationAnti-inflammatory |
| spellingShingle | Fuxia Hu Chao Liu Fengqin Wang Changxin Zhou Maotong Zhu Dongxiao Sun-Waterhouse Zhaosheng Wang Phenolic compounds from Chaenomeles speciosa alleviate inflammation in lipopolysaccharide-treated RAW264.7 macrophages via the NF-κB and MAPK pathways Food Science and Human Wellness Chaenomeles speciosa Phenolic extract Purification Anti-inflammatory |
| title | Phenolic compounds from Chaenomeles speciosa alleviate inflammation in lipopolysaccharide-treated RAW264.7 macrophages via the NF-κB and MAPK pathways |
| title_full | Phenolic compounds from Chaenomeles speciosa alleviate inflammation in lipopolysaccharide-treated RAW264.7 macrophages via the NF-κB and MAPK pathways |
| title_fullStr | Phenolic compounds from Chaenomeles speciosa alleviate inflammation in lipopolysaccharide-treated RAW264.7 macrophages via the NF-κB and MAPK pathways |
| title_full_unstemmed | Phenolic compounds from Chaenomeles speciosa alleviate inflammation in lipopolysaccharide-treated RAW264.7 macrophages via the NF-κB and MAPK pathways |
| title_short | Phenolic compounds from Chaenomeles speciosa alleviate inflammation in lipopolysaccharide-treated RAW264.7 macrophages via the NF-κB and MAPK pathways |
| title_sort | phenolic compounds from chaenomeles speciosa alleviate inflammation in lipopolysaccharide treated raw264 7 macrophages via the nf κb and mapk pathways |
| topic | Chaenomeles speciosa Phenolic extract Purification Anti-inflammatory |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002440 |
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