Reclamation of Herb Residues Using Probiotics and Their Therapeutic Effect on Diarrhea
Residues from herbal medicine processing in pharmaceutical plants create a large amount of waste (herb residues), which consists mainly of environmental pollution and medicinal waste. In order to resolve this problem, probiotics of Bacillus (B.) subtilis, Aspergillus (A.) oryzae, and Lactobacillus (...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/4265898 |
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| author | Fanjing Meng Tingtao Chen Dongwen Ma Xin Wang Xiaoxiao Zhao Puyuan Tian Huan Wang Zhiwen Hai Liang Shen Xianyao Tang Xiaolei Wang Hongbo Xin |
| author_facet | Fanjing Meng Tingtao Chen Dongwen Ma Xin Wang Xiaoxiao Zhao Puyuan Tian Huan Wang Zhiwen Hai Liang Shen Xianyao Tang Xiaolei Wang Hongbo Xin |
| author_sort | Fanjing Meng |
| collection | DOAJ |
| description | Residues from herbal medicine processing in pharmaceutical plants create a large amount of waste (herb residues), which consists mainly of environmental pollution and medicinal waste. In order to resolve this problem, probiotics of Bacillus (B.) subtilis, Aspergillus (A.) oryzae, and Lactobacillus (L.) plantarum M3 are selected to reuse herb residue of Jianweixiaoshi tablets (JT), and an antibiotic-associated diarrhea (AAD) mouse model was established to evaluate the therapeutic effects of the herb residue fermentation supernatant. Our results indicated that the fermentation supernatant had scavenged 77.8% of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 78% of O2•−, 36.7% of •OH, 39% of Fe2+ chelation, and 716 mg/L reducing power. The inhibition zones for Salmonella (S.) typhimurium, S. enteritidis, Shigella (Sh.) flexneri, Escherichia (E.) coli, Listeria (L.) monocytogenes, Sh. dysenteriae 301, and Staphylococcus (S.) aureus were 17, 14, 19, 18, 20, 19, and 20 mm, respectively. The in vivo results indicated that the fermentation supernatant resulted in a high diarrhea inhibition rate (56%, p<0.05), greatly enhanced the disruption of bacterial diversity caused by antibiotics, and restored the dominant position of L. johnsonii in the treatment and recovery stages. Therefore, the combination of the herb residue and probiotics suggests a potential to explore conversion of these materials for the possible development of therapies for AAD. |
| format | Article |
| id | doaj-art-8bb9a2344a3e437d8b8268de8cc7e8d2 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-8bb9a2344a3e437d8b8268de8cc7e8d22025-08-20T03:37:41ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/42658984265898Reclamation of Herb Residues Using Probiotics and Their Therapeutic Effect on DiarrheaFanjing Meng0Tingtao Chen1Dongwen Ma2Xin Wang3Xiaoxiao Zhao4Puyuan Tian5Huan Wang6Zhiwen Hai7Liang Shen8Xianyao Tang9Xiaolei Wang10Hongbo Xin11Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, ChinaInstitute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, ChinaInstitute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, ChinaInstitute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, ChinaInstitute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, ChinaInstitute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, ChinaInstitute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, ChinaDepartment of Obstetrics and Gynaecology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250012, ChinaInstitute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, ChinaInstitute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, ChinaInstitute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, ChinaResidues from herbal medicine processing in pharmaceutical plants create a large amount of waste (herb residues), which consists mainly of environmental pollution and medicinal waste. In order to resolve this problem, probiotics of Bacillus (B.) subtilis, Aspergillus (A.) oryzae, and Lactobacillus (L.) plantarum M3 are selected to reuse herb residue of Jianweixiaoshi tablets (JT), and an antibiotic-associated diarrhea (AAD) mouse model was established to evaluate the therapeutic effects of the herb residue fermentation supernatant. Our results indicated that the fermentation supernatant had scavenged 77.8% of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 78% of O2•−, 36.7% of •OH, 39% of Fe2+ chelation, and 716 mg/L reducing power. The inhibition zones for Salmonella (S.) typhimurium, S. enteritidis, Shigella (Sh.) flexneri, Escherichia (E.) coli, Listeria (L.) monocytogenes, Sh. dysenteriae 301, and Staphylococcus (S.) aureus were 17, 14, 19, 18, 20, 19, and 20 mm, respectively. The in vivo results indicated that the fermentation supernatant resulted in a high diarrhea inhibition rate (56%, p<0.05), greatly enhanced the disruption of bacterial diversity caused by antibiotics, and restored the dominant position of L. johnsonii in the treatment and recovery stages. Therefore, the combination of the herb residue and probiotics suggests a potential to explore conversion of these materials for the possible development of therapies for AAD.http://dx.doi.org/10.1155/2017/4265898 |
| spellingShingle | Fanjing Meng Tingtao Chen Dongwen Ma Xin Wang Xiaoxiao Zhao Puyuan Tian Huan Wang Zhiwen Hai Liang Shen Xianyao Tang Xiaolei Wang Hongbo Xin Reclamation of Herb Residues Using Probiotics and Their Therapeutic Effect on Diarrhea Mediators of Inflammation |
| title | Reclamation of Herb Residues Using Probiotics and Their Therapeutic Effect on Diarrhea |
| title_full | Reclamation of Herb Residues Using Probiotics and Their Therapeutic Effect on Diarrhea |
| title_fullStr | Reclamation of Herb Residues Using Probiotics and Their Therapeutic Effect on Diarrhea |
| title_full_unstemmed | Reclamation of Herb Residues Using Probiotics and Their Therapeutic Effect on Diarrhea |
| title_short | Reclamation of Herb Residues Using Probiotics and Their Therapeutic Effect on Diarrhea |
| title_sort | reclamation of herb residues using probiotics and their therapeutic effect on diarrhea |
| url | http://dx.doi.org/10.1155/2017/4265898 |
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