Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells.
<h4>Objective</h4>Adipose Tissue Stromal Cells (ASCs) have important clinical applications in the regenerative medicine, cell replacement and gene therapies. Subcutaneous Adipose Tissue (SAT) is the most common source of these cells. The adult human thymus degenerates into adipose tissue...
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0144401&type=printable |
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| author | Wilfredo Oliva-Olivera Leticia Coín-Aragüez Julián Salas Said Lhamyani Adriana-Mariel Gentile Esteban Sarria García Abdelkrim Hmadcha Hatem Zayed Antonio Vega-Rioja Francisco J Tinahones Rajaa El Bekay |
| author_facet | Wilfredo Oliva-Olivera Leticia Coín-Aragüez Julián Salas Said Lhamyani Adriana-Mariel Gentile Esteban Sarria García Abdelkrim Hmadcha Hatem Zayed Antonio Vega-Rioja Francisco J Tinahones Rajaa El Bekay |
| author_sort | Wilfredo Oliva-Olivera |
| collection | DOAJ |
| description | <h4>Objective</h4>Adipose Tissue Stromal Cells (ASCs) have important clinical applications in the regenerative medicine, cell replacement and gene therapies. Subcutaneous Adipose Tissue (SAT) is the most common source of these cells. The adult human thymus degenerates into adipose tissue (TAT). However, it has never been studied before as a source of stem cells.<h4>Material and methods</h4>We performed a comparative characterization of TAT-ASCs and SAT-ASCs from myocardial ischemic subjects (n = 32) according to the age of the subjects.<h4>Results</h4>TAT-ASCs and SAT-ASCs showed similar features regarding their adherence, morphology and in their capacity to form CFU-F. Moreover, they have the capacity to differentiate into osteocyte and adipocyte lineages; and they present a surface marker profile corresponding with stem cells derived from AT; CD73+CD90+CD105+CD14-CD19-CD45-HLA-DR. Interestingly, and in opposition to SAT-ASCs, TAT-ASCs have CD14+CD34+CD133+CD45- cells. Moreover, TAT-ASCs from elderly subjects showed higher adipogenic and osteogenic capacities compared to middle aged subjects, indicating that, rather than impairing; aging seems to increase adipogenic and osteogenic capacities of TAT-ASCs.<h4>Conclusions</h4>This study describes the human TAT as a source of mesenchymal stem cells, which may have an enormous potential for regenerative medicine. |
| format | Article |
| id | doaj-art-8bb7318bc9754f2d93b6d4a2e377dafd |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-8bb7318bc9754f2d93b6d4a2e377dafd2025-08-20T02:15:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011012e014440110.1371/journal.pone.0144401Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells.Wilfredo Oliva-OliveraLeticia Coín-AragüezJulián SalasSaid LhamyaniAdriana-Mariel GentileEsteban Sarria GarcíaAbdelkrim HmadchaHatem ZayedAntonio Vega-RiojaFrancisco J TinahonesRajaa El Bekay<h4>Objective</h4>Adipose Tissue Stromal Cells (ASCs) have important clinical applications in the regenerative medicine, cell replacement and gene therapies. Subcutaneous Adipose Tissue (SAT) is the most common source of these cells. The adult human thymus degenerates into adipose tissue (TAT). However, it has never been studied before as a source of stem cells.<h4>Material and methods</h4>We performed a comparative characterization of TAT-ASCs and SAT-ASCs from myocardial ischemic subjects (n = 32) according to the age of the subjects.<h4>Results</h4>TAT-ASCs and SAT-ASCs showed similar features regarding their adherence, morphology and in their capacity to form CFU-F. Moreover, they have the capacity to differentiate into osteocyte and adipocyte lineages; and they present a surface marker profile corresponding with stem cells derived from AT; CD73+CD90+CD105+CD14-CD19-CD45-HLA-DR. Interestingly, and in opposition to SAT-ASCs, TAT-ASCs have CD14+CD34+CD133+CD45- cells. Moreover, TAT-ASCs from elderly subjects showed higher adipogenic and osteogenic capacities compared to middle aged subjects, indicating that, rather than impairing; aging seems to increase adipogenic and osteogenic capacities of TAT-ASCs.<h4>Conclusions</h4>This study describes the human TAT as a source of mesenchymal stem cells, which may have an enormous potential for regenerative medicine.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0144401&type=printable |
| spellingShingle | Wilfredo Oliva-Olivera Leticia Coín-Aragüez Julián Salas Said Lhamyani Adriana-Mariel Gentile Esteban Sarria García Abdelkrim Hmadcha Hatem Zayed Antonio Vega-Rioja Francisco J Tinahones Rajaa El Bekay Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells. PLoS ONE |
| title | Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells. |
| title_full | Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells. |
| title_fullStr | Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells. |
| title_full_unstemmed | Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells. |
| title_short | Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells. |
| title_sort | myocardial ischemic subject s thymus fat a novel source of multipotent stromal cells |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0144401&type=printable |
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