Use of ultra-freezers of -152 °c as a viable alternative to liquid nitrogen in storing
<b>Aim: </b>This study aims to investigate the utilization of the ultra-freezer to store the semen at a -152 °C as an alternative method to traditional semen storage methods.<p> <b>Materials and Methods: </b>A total of 80 straws were collected from two bulls on differen...
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Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1363 |
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| author | Israa Mahmoud Alhamad Mehmet Borga Tirpan |
| author_facet | Israa Mahmoud Alhamad Mehmet Borga Tirpan |
| author_sort | Israa Mahmoud Alhamad |
| collection | DOAJ |
| description | <b>Aim: </b>This study aims to investigate the utilization of the ultra-freezer to store
the semen at a -152 °C as an alternative method to traditional semen storage
methods.<p>
<b>Materials and Methods: </b>A total of 80 straws were collected from two bulls
on different days. The semen collected on the same day were sorted into two
different groups. The experimental group of the study was composed of sperm
stored at -152 °C for one week, while the control group was composed of
sperm stored at -196 °C using the traditional storage method. At the end of the
experimental period, frozen semen at -196 °C and -152 °C were thawed at a
temperature of 37 °C water bath for 30 seconds. Then, the spermatological parameters
and morphological properties of the sperm were examined by using
a computer-assisted sperm analysis system (CASA).<p>
<b>Results: </b>The evaluation of semen samples yielded the following results; the
motility and progressive motility of the frozen and stored sperm (69.66%;
37.61%) at -196 °C were higher than the values (54.54%; 28.72%) stored at
-152 °C (p<0.001). The percentage of sperm with hyperactivity in group -196
and -152, was found to be 30.79% and 25.61%, respectively, (p<0.05). There
was no statistical difference between kinetic values, and it was close to numerical
values (p>0.05). Moreover, it was determined that the abnormal sperm
rate was at 32% for the semen stored in liquid nitrogen and was 45% for the
semen stored at -152 °C (p <0.001).<p>
<b>Conclusion: </b>As a result of the experiment, it was determined that the postthawing
parameters of the sperms stored in -152 °C freezer were within acceptable
ranges. |
| format | Article |
| id | doaj-art-8baec4fffa5d4238b523e0aaa868c758 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-8baec4fffa5d4238b523e0aaa868c7582025-02-03T11:25:37ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19533731861921363Use of ultra-freezers of -152 °c as a viable alternative to liquid nitrogen in storingIsraa Mahmoud AlhamadMehmet Borga Tirpan<b>Aim: </b>This study aims to investigate the utilization of the ultra-freezer to store the semen at a -152 °C as an alternative method to traditional semen storage methods.<p> <b>Materials and Methods: </b>A total of 80 straws were collected from two bulls on different days. The semen collected on the same day were sorted into two different groups. The experimental group of the study was composed of sperm stored at -152 °C for one week, while the control group was composed of sperm stored at -196 °C using the traditional storage method. At the end of the experimental period, frozen semen at -196 °C and -152 °C were thawed at a temperature of 37 °C water bath for 30 seconds. Then, the spermatological parameters and morphological properties of the sperm were examined by using a computer-assisted sperm analysis system (CASA).<p> <b>Results: </b>The evaluation of semen samples yielded the following results; the motility and progressive motility of the frozen and stored sperm (69.66%; 37.61%) at -196 °C were higher than the values (54.54%; 28.72%) stored at -152 °C (p<0.001). The percentage of sperm with hyperactivity in group -196 and -152, was found to be 30.79% and 25.61%, respectively, (p<0.05). There was no statistical difference between kinetic values, and it was close to numerical values (p>0.05). Moreover, it was determined that the abnormal sperm rate was at 32% for the semen stored in liquid nitrogen and was 45% for the semen stored at -152 °C (p <0.001).<p> <b>Conclusion: </b>As a result of the experiment, it was determined that the postthawing parameters of the sperms stored in -152 °C freezer were within acceptable ranges.http://eurasianjvetsci.org/pdf.php3?id=1363bull semenstorage-152°c ultra -low temperature freezer |
| spellingShingle | Israa Mahmoud Alhamad Mehmet Borga Tirpan Use of ultra-freezers of -152 °c as a viable alternative to liquid nitrogen in storing Eurasian Journal of Veterinary Sciences bull semen storage -152°c ultra -low temperature freezer |
| title | Use of ultra-freezers of -152 °c as a viable alternative to liquid nitrogen in storing |
| title_full | Use of ultra-freezers of -152 °c as a viable alternative to liquid nitrogen in storing |
| title_fullStr | Use of ultra-freezers of -152 °c as a viable alternative to liquid nitrogen in storing |
| title_full_unstemmed | Use of ultra-freezers of -152 °c as a viable alternative to liquid nitrogen in storing |
| title_short | Use of ultra-freezers of -152 °c as a viable alternative to liquid nitrogen in storing |
| title_sort | use of ultra freezers of 152 °c as a viable alternative to liquid nitrogen in storing |
| topic | bull semen storage -152°c ultra -low temperature freezer |
| url | http://eurasianjvetsci.org/pdf.php3?id=1363 |
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