Metamorphosis and development of malaria parasites in the liver are regulated by unconventional autophagy

Malaria parasites encounter diverse conditions as they transition between mosquito and mammalian hosts. A characteristic of the sporozoite stage of the parasite is that once it enters the hepatocyte, it changes its morphology and metabolism. Motile-elongated sporozoites transform into round trophozo...

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Main Authors: Suryansh Rajput, Satish Mishra
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Autophagy Reports
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Online Access:https://www.tandfonline.com/doi/10.1080/27694127.2025.2504060
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author Suryansh Rajput
Satish Mishra
author_facet Suryansh Rajput
Satish Mishra
author_sort Suryansh Rajput
collection DOAJ
description Malaria parasites encounter diverse conditions as they transition between mosquito and mammalian hosts. A characteristic of the sporozoite stage of the parasite is that once it enters the hepatocyte, it changes its morphology and metabolism. Motile-elongated sporozoites transform into round trophozoites, discard unnecessary organelles, undergo extensive replication, and mature into hepatic merozoites. However, the mechanisms of superfluous organelle elimination and apicoplast biogenesis are unclear. In our latest study, using a conditional mutagenesis system, we clarified the role of Atg7 during parasite metamorphosis in the liver. We found that cytosolic Atg7 is essential for the localization of Atg8 on the membrane and the development of parasites in the blood and liver stages. Parasites lacking Atg7 fail to lipidate Atg8 on the membrane, which leads to impaired exocytosis of micronemes, and parasites eventually fail to mature into hepatic merozoites. This work provides insights into the essential role of Atg7 in maintaining parasite cellular homeostasis during liver stage development.
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spelling doaj-art-8baca86a6e514d199096353756db06df2025-08-20T03:53:12ZengTaylor & Francis GroupAutophagy Reports2769-41272025-12-014110.1080/27694127.2025.2504060Metamorphosis and development of malaria parasites in the liver are regulated by unconventional autophagySuryansh Rajput0Satish Mishra1Division of Molecular Microbiology and Immunology, CSIR-Central Drug Research Institute, Lucknow, IndiaDivision of Molecular Microbiology and Immunology, CSIR-Central Drug Research Institute, Lucknow, IndiaMalaria parasites encounter diverse conditions as they transition between mosquito and mammalian hosts. A characteristic of the sporozoite stage of the parasite is that once it enters the hepatocyte, it changes its morphology and metabolism. Motile-elongated sporozoites transform into round trophozoites, discard unnecessary organelles, undergo extensive replication, and mature into hepatic merozoites. However, the mechanisms of superfluous organelle elimination and apicoplast biogenesis are unclear. In our latest study, using a conditional mutagenesis system, we clarified the role of Atg7 during parasite metamorphosis in the liver. We found that cytosolic Atg7 is essential for the localization of Atg8 on the membrane and the development of parasites in the blood and liver stages. Parasites lacking Atg7 fail to lipidate Atg8 on the membrane, which leads to impaired exocytosis of micronemes, and parasites eventually fail to mature into hepatic merozoites. This work provides insights into the essential role of Atg7 in maintaining parasite cellular homeostasis during liver stage development.https://www.tandfonline.com/doi/10.1080/27694127.2025.2504060ApicoplastautophagyAtg7Atg8lipidationliver stage
spellingShingle Suryansh Rajput
Satish Mishra
Metamorphosis and development of malaria parasites in the liver are regulated by unconventional autophagy
Autophagy Reports
Apicoplast
autophagy
Atg7
Atg8
lipidation
liver stage
title Metamorphosis and development of malaria parasites in the liver are regulated by unconventional autophagy
title_full Metamorphosis and development of malaria parasites in the liver are regulated by unconventional autophagy
title_fullStr Metamorphosis and development of malaria parasites in the liver are regulated by unconventional autophagy
title_full_unstemmed Metamorphosis and development of malaria parasites in the liver are regulated by unconventional autophagy
title_short Metamorphosis and development of malaria parasites in the liver are regulated by unconventional autophagy
title_sort metamorphosis and development of malaria parasites in the liver are regulated by unconventional autophagy
topic Apicoplast
autophagy
Atg7
Atg8
lipidation
liver stage
url https://www.tandfonline.com/doi/10.1080/27694127.2025.2504060
work_keys_str_mv AT suryanshrajput metamorphosisanddevelopmentofmalariaparasitesintheliverareregulatedbyunconventionalautophagy
AT satishmishra metamorphosisanddevelopmentofmalariaparasitesintheliverareregulatedbyunconventionalautophagy