Akabane virus isolated from biting midges and its infection in local domestic animal, Yunnan, China: a field and laboratory investigation
IntroductionWe verified that Akabane virus (AKAV) is transmitted through biting midges and infects local domestic animals.MethodsIn 2013, viruses were isolate from biting midges in Yunnan, China, using BHK-21 and C6/36 cells. Two AKAV strains (No. 52 and 55) that induced cytopathogenic effects (CPE)...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| author | Jinxin Meng Yuwen He Nan Li Zhenxing Yang Si Fu Dongmei Wang Aiguo Xin Jinglin Wang Guodong Liang |
| author_facet | Jinxin Meng Yuwen He Nan Li Zhenxing Yang Si Fu Dongmei Wang Aiguo Xin Jinglin Wang Guodong Liang |
| author_sort | Jinxin Meng |
| collection | DOAJ |
| description | IntroductionWe verified that Akabane virus (AKAV) is transmitted through biting midges and infects local domestic animals.MethodsIn 2013, viruses were isolate from biting midges in Yunnan, China, using BHK-21 and C6/36 cells. Two AKAV strains (No. 52 and 55) that induced cytopathogenic effects (CPE) in BHK-21, MDBK, and Vero cells were characterized.ResultsThe complete genomic sequence of both viruses consisted three RNA segments (S, M, and L). The S segment (856 nucleotides) encoded a 233-amino-acid nucleocapsid protein and a 91-amino-acid nonstructural protein, while the M segment (4309 nucleotides) encoded a 1401-amino-acid polyprotein. The L segment (6869 nucleotides) encoded a 2511-amino-acid RNA-dependent RNA polymerase. Phylogenetic analysis revealed that specimen Nos. 52 and 55 clustered with AKAV genotype Ia viruses isolated from Asia. The AKAV strain (55) neutralizing antibody exhibited a total positive rate of 43.55% (202/466) against serum samples from cattle and goats collected in Yunnan Province. Specifically, the positive rates were 48.77% (139/285) for cattle and 34.81% (63/181) for goats. Neutralizing antibody titers in cattle (1:32–1:128) were higher than those in goats (1:4–1:16).DiscussionThis study represents the first isolation of AKAV from biting midges in China, along with the detection of high neutralizing antibody titers against AKAV in the serum samples of local cattle and goats. These findings suggested that biting midges are involved in AKAV transmission among domestic animals in Yunnan Province, China. |
| format | Article |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-8ba9c89981df4bdf9b4e9999dc23189e2025-08-20T02:59:38ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-01-011410.3389/fcimb.2024.14340451434045Akabane virus isolated from biting midges and its infection in local domestic animal, Yunnan, China: a field and laboratory investigationJinxin Meng0Yuwen He1Nan Li2Zhenxing Yang3Si Fu4Dongmei Wang5Aiguo Xin6Jinglin Wang7Guodong Liang8Yunnan Tropical and Subtropical Animal Viral Disease Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan, ChinaYunnan Tropical and Subtropical Animal Viral Disease Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan, ChinaYunnan Tropical and Subtropical Animal Viral Disease Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan, ChinaYunnan Tropical and Subtropical Animal Viral Disease Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan, ChinaJiangcheng County Animal Disease Prevention and Control Center, Jiangcheng, Yunnan, ChinaJiangcheng County Animal Disease Prevention and Control Center, Jiangcheng, Yunnan, ChinaYunnan Tropical and Subtropical Animal Viral Disease Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan, ChinaYunnan Tropical and Subtropical Animal Viral Disease Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan, ChinaNational Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaIntroductionWe verified that Akabane virus (AKAV) is transmitted through biting midges and infects local domestic animals.MethodsIn 2013, viruses were isolate from biting midges in Yunnan, China, using BHK-21 and C6/36 cells. Two AKAV strains (No. 52 and 55) that induced cytopathogenic effects (CPE) in BHK-21, MDBK, and Vero cells were characterized.ResultsThe complete genomic sequence of both viruses consisted three RNA segments (S, M, and L). The S segment (856 nucleotides) encoded a 233-amino-acid nucleocapsid protein and a 91-amino-acid nonstructural protein, while the M segment (4309 nucleotides) encoded a 1401-amino-acid polyprotein. The L segment (6869 nucleotides) encoded a 2511-amino-acid RNA-dependent RNA polymerase. Phylogenetic analysis revealed that specimen Nos. 52 and 55 clustered with AKAV genotype Ia viruses isolated from Asia. The AKAV strain (55) neutralizing antibody exhibited a total positive rate of 43.55% (202/466) against serum samples from cattle and goats collected in Yunnan Province. Specifically, the positive rates were 48.77% (139/285) for cattle and 34.81% (63/181) for goats. Neutralizing antibody titers in cattle (1:32–1:128) were higher than those in goats (1:4–1:16).DiscussionThis study represents the first isolation of AKAV from biting midges in China, along with the detection of high neutralizing antibody titers against AKAV in the serum samples of local cattle and goats. These findings suggested that biting midges are involved in AKAV transmission among domestic animals in Yunnan Province, China.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1434045/fullAkabane virusbiting midgesdomestic animalvirus isolationneutralizing antibody |
| spellingShingle | Jinxin Meng Yuwen He Nan Li Zhenxing Yang Si Fu Dongmei Wang Aiguo Xin Jinglin Wang Guodong Liang Akabane virus isolated from biting midges and its infection in local domestic animal, Yunnan, China: a field and laboratory investigation Frontiers in Cellular and Infection Microbiology Akabane virus biting midges domestic animal virus isolation neutralizing antibody |
| title | Akabane virus isolated from biting midges and its infection in local domestic animal, Yunnan, China: a field and laboratory investigation |
| title_full | Akabane virus isolated from biting midges and its infection in local domestic animal, Yunnan, China: a field and laboratory investigation |
| title_fullStr | Akabane virus isolated from biting midges and its infection in local domestic animal, Yunnan, China: a field and laboratory investigation |
| title_full_unstemmed | Akabane virus isolated from biting midges and its infection in local domestic animal, Yunnan, China: a field and laboratory investigation |
| title_short | Akabane virus isolated from biting midges and its infection in local domestic animal, Yunnan, China: a field and laboratory investigation |
| title_sort | akabane virus isolated from biting midges and its infection in local domestic animal yunnan china a field and laboratory investigation |
| topic | Akabane virus biting midges domestic animal virus isolation neutralizing antibody |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1434045/full |
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