Histopathological analysis of the liver in hypercholesterolemia rats treated with Dillenia serrata fruits
Background: Dillenia serrata (locally known as singi) is a natural product with the potential to improve liver function in hypercholesterolemia, due to anticholesterol and antioxidant properties. Objective: This study aimed to investigate the effect of D. serrata on the histopathological feature...
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Indonesian Society for Biochemistry and Molecular Biology
2022-12-01
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| Series: | Acta Biochimica Indonesiana |
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| Online Access: | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/85 |
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| author | Tien Tri Wistya Utami Pranita Aritrina Laode Kardin Sukurni Andi Noor Kholidha Syarifin |
| author_facet | Tien Tri Wistya Utami Pranita Aritrina Laode Kardin Sukurni Andi Noor Kholidha Syarifin |
| author_sort | Tien |
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Background: Dillenia serrata (locally known as singi) is a natural product with the potential to improve liver function in hypercholesterolemia, due to anticholesterol and antioxidant properties.
Objective: This study aimed to investigate the effect of D. serrata on the histopathological features of rat livers induced by a high-fat diet.
Methods: This study used a quasi-experimental in vivo with a post-test-only control group design. Rats were divided into four groups: normal, high-fat diet, as well as D. serrata, and simvastatin treatment. Hypercholesterolemia was induced by a high-fat diet for two weeks. Rat liver tissues were analyzed histologically using Hematoxylin-Eosin (HE) staining, and were observed under a light microscope at 40x magnification in five wide fields of view.
Results: The high-fat diet group had the most adipose cells, while the D. serrata group had the least. Statistical analysis demonstrated a significant difference between the high-fat diet group and the other groups (p < 0.05). Interestingly, no significant difference between the D. serrata group and either the normal or simvastatin group (p > 0.05), suggesting the treatment of fruit may restore liver function comparable to normal and simvastatin group.
Conclusion: The D. serrata fruit reduces the number of fat cells in the histopathology of rats induced with the high-fat diet.
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| format | Article |
| id | doaj-art-8b9ed13a8592489b9b042e4e1d931564 |
| institution | Kabale University |
| issn | 2654-6108 2654-3222 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Indonesian Society for Biochemistry and Molecular Biology |
| record_format | Article |
| series | Acta Biochimica Indonesiana |
| spelling | doaj-art-8b9ed13a8592489b9b042e4e1d9315642025-02-08T03:05:58ZengIndonesian Society for Biochemistry and Molecular BiologyActa Biochimica Indonesiana2654-61082654-32222022-12-015210.32889/actabioina.56Histopathological analysis of the liver in hypercholesterolemia rats treated with Dillenia serrata fruitsTien0Tri Wistya Utami1Pranita Aritrina2Laode Kardin3Sukurni4Andi Noor Kholidha Syarifin5Department of Biochemistry, Faculty of Medicine, Univeristas Halu Oleo, Kendari, Sulawesi Tenggara, IndonesiaFaculty of Medicine, Universitas Halu Oleo, Kendari, Sulawesi Tenggara, IndonesiaDepartment of Biochemistry, Faculty of Medicine, Univeristas Halu Oleo, Kendari, Sulawesi Tenggara, IndonesiaDepartment of Internal Medicine, Faculty of Medicine, Univeristas Halu Oleo, Kendari, Sulawesi TenggaraDepartment of Nursing, Faculty of Medicine, Univeristas Halu Oleo, Kendari, Sulawesi Tenggara, IndonesiaDepartment of Biochemistry, Faculty of Medicine, Univeristas Halu Oleo, Kendari, Sulawesi Tenggara, Indonesia Background: Dillenia serrata (locally known as singi) is a natural product with the potential to improve liver function in hypercholesterolemia, due to anticholesterol and antioxidant properties. Objective: This study aimed to investigate the effect of D. serrata on the histopathological features of rat livers induced by a high-fat diet. Methods: This study used a quasi-experimental in vivo with a post-test-only control group design. Rats were divided into four groups: normal, high-fat diet, as well as D. serrata, and simvastatin treatment. Hypercholesterolemia was induced by a high-fat diet for two weeks. Rat liver tissues were analyzed histologically using Hematoxylin-Eosin (HE) staining, and were observed under a light microscope at 40x magnification in five wide fields of view. Results: The high-fat diet group had the most adipose cells, while the D. serrata group had the least. Statistical analysis demonstrated a significant difference between the high-fat diet group and the other groups (p < 0.05). Interestingly, no significant difference between the D. serrata group and either the normal or simvastatin group (p > 0.05), suggesting the treatment of fruit may restore liver function comparable to normal and simvastatin group. Conclusion: The D. serrata fruit reduces the number of fat cells in the histopathology of rats induced with the high-fat diet. https://pbbmi.org/newjurnal/index.php/actabioina/article/view/85Dillenia serratanon-alcoholic fatty liver diseasehigh fat diethistopathological of liver |
| spellingShingle | Tien Tri Wistya Utami Pranita Aritrina Laode Kardin Sukurni Andi Noor Kholidha Syarifin Histopathological analysis of the liver in hypercholesterolemia rats treated with Dillenia serrata fruits Acta Biochimica Indonesiana Dillenia serrata non-alcoholic fatty liver disease high fat diet histopathological of liver |
| title | Histopathological analysis of the liver in hypercholesterolemia rats treated with Dillenia serrata fruits |
| title_full | Histopathological analysis of the liver in hypercholesterolemia rats treated with Dillenia serrata fruits |
| title_fullStr | Histopathological analysis of the liver in hypercholesterolemia rats treated with Dillenia serrata fruits |
| title_full_unstemmed | Histopathological analysis of the liver in hypercholesterolemia rats treated with Dillenia serrata fruits |
| title_short | Histopathological analysis of the liver in hypercholesterolemia rats treated with Dillenia serrata fruits |
| title_sort | histopathological analysis of the liver in hypercholesterolemia rats treated with dillenia serrata fruits |
| topic | Dillenia serrata non-alcoholic fatty liver disease high fat diet histopathological of liver |
| url | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/85 |
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