Proteomics and Recurrence of Atrial Fibrillation: A Pilot Study Nested in the PREDIMAR Trial

Introduction: Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia worldwide. Although catheter ablation is the most efficacious therapy, relapses occur frequently (30%) in the first year after ablation. Novel biomarkers of recurrence are needed for a better prediction...

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Main Authors: Cristina Razquin, Joaquín Fernandez-Irigoyen, María Teresa Barrio-López, Begoña López, Susana Ravassa, Pablo Ramos, Rosa Macías-Ruíz, Alicia Ibañez Criado, Enrique Santamaría, Leticia Goni, Eduardo Castellanos, Jose Luis Ibañez Criado, Luis Tercedor, Ignacio García-Bolao, Miguel A. Martínez-González, Jesús Almendral, Miguel Ruiz-Canela
Format: Article
Language:English
Published: Karger Publishers 2025-01-01
Series:Lifestyle Genomics
Online Access:https://karger.com/article/doi/10.1159/000543639
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author Cristina Razquin
Joaquín Fernandez-Irigoyen
María Teresa Barrio-López
Begoña López
Susana Ravassa
Pablo Ramos
Rosa Macías-Ruíz
Alicia Ibañez Criado
Enrique Santamaría
Leticia Goni
Eduardo Castellanos
Jose Luis Ibañez Criado
Luis Tercedor
Ignacio García-Bolao
Miguel A. Martínez-González
Jesús Almendral
Miguel Ruiz-Canela
author_facet Cristina Razquin
Joaquín Fernandez-Irigoyen
María Teresa Barrio-López
Begoña López
Susana Ravassa
Pablo Ramos
Rosa Macías-Ruíz
Alicia Ibañez Criado
Enrique Santamaría
Leticia Goni
Eduardo Castellanos
Jose Luis Ibañez Criado
Luis Tercedor
Ignacio García-Bolao
Miguel A. Martínez-González
Jesús Almendral
Miguel Ruiz-Canela
author_sort Cristina Razquin
collection DOAJ
description Introduction: Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia worldwide. Although catheter ablation is the most efficacious therapy, relapses occur frequently (30%) in the first year after ablation. Novel biomarkers of recurrence are needed for a better prediction of recurrence and management of AF. In this pilot study, we aimed to analyze the baseline proteome of subjects included in a case-control study to find differential proteins associated with AF recurrence. Methods: Baseline serum proteomics (354 proteins) data from 16 cases (recurrent AF) and 17 controls (non-recurrent) were obtained using MS/MS analysis. A false discovery rate was performed using a nonlinear fitting method for the selection of proteins. Logistic regression models were used to further analyze the association between differentially expressed proteins and AF recurrence. Results: Ten proteins were differentially represented in cases vs. controls. Two were upregulated in the cases compared to the controls: keratin type I cytoskeletal 17 (Fold-change [FC] = 2.14; p = 0.017) and endoplasmic bifunctional protein (FC = 1.65; p = 0.032). Eight were downregulated in the cases compared to the controls: C4bpA (FC = 0.64; p = 0.006), coagulation factor XI (FC = 0.83; p = 0.011), CLIC1 (FC = 0.62; p = 0.017), haptoglobin (FC = 0.37; p = 0.021), Ig alpha-2 chain C region (FC = 0.49; p = 0.022), C4bpB (FC = 0.73; p = 0.028), N-acetylglucosamine-1-phosphotransferase subunit gamma (FC = 0.61; p = 0.033), and platelet glycoprotein Ib alpha chain (FC = 0.84; p = 0.038). Conclusion: This pilot study identifies ten differentially expressed serum proteins associated with AF recurrence, offering potential biomarkers for improved prediction and management.
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spelling doaj-art-8b9eaa5e71024a2abaf6543c912e94972025-08-20T03:09:48ZengKarger PublishersLifestyle Genomics2504-31882025-01-01181525810.1159/000543639Proteomics and Recurrence of Atrial Fibrillation: A Pilot Study Nested in the PREDIMAR TrialCristina RazquinJoaquín Fernandez-IrigoyenMaría Teresa Barrio-LópezBegoña LópezSusana RavassaPablo RamosRosa Macías-RuízAlicia Ibañez CriadoEnrique SantamaríaLeticia GoniEduardo CastellanosJose Luis Ibañez CriadoLuis TercedorIgnacio García-BolaoMiguel A. Martínez-GonzálezJesús AlmendralMiguel Ruiz-Canela Introduction: Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia worldwide. Although catheter ablation is the most efficacious therapy, relapses occur frequently (30%) in the first year after ablation. Novel biomarkers of recurrence are needed for a better prediction of recurrence and management of AF. In this pilot study, we aimed to analyze the baseline proteome of subjects included in a case-control study to find differential proteins associated with AF recurrence. Methods: Baseline serum proteomics (354 proteins) data from 16 cases (recurrent AF) and 17 controls (non-recurrent) were obtained using MS/MS analysis. A false discovery rate was performed using a nonlinear fitting method for the selection of proteins. Logistic regression models were used to further analyze the association between differentially expressed proteins and AF recurrence. Results: Ten proteins were differentially represented in cases vs. controls. Two were upregulated in the cases compared to the controls: keratin type I cytoskeletal 17 (Fold-change [FC] = 2.14; p = 0.017) and endoplasmic bifunctional protein (FC = 1.65; p = 0.032). Eight were downregulated in the cases compared to the controls: C4bpA (FC = 0.64; p = 0.006), coagulation factor XI (FC = 0.83; p = 0.011), CLIC1 (FC = 0.62; p = 0.017), haptoglobin (FC = 0.37; p = 0.021), Ig alpha-2 chain C region (FC = 0.49; p = 0.022), C4bpB (FC = 0.73; p = 0.028), N-acetylglucosamine-1-phosphotransferase subunit gamma (FC = 0.61; p = 0.033), and platelet glycoprotein Ib alpha chain (FC = 0.84; p = 0.038). Conclusion: This pilot study identifies ten differentially expressed serum proteins associated with AF recurrence, offering potential biomarkers for improved prediction and management. https://karger.com/article/doi/10.1159/000543639
spellingShingle Cristina Razquin
Joaquín Fernandez-Irigoyen
María Teresa Barrio-López
Begoña López
Susana Ravassa
Pablo Ramos
Rosa Macías-Ruíz
Alicia Ibañez Criado
Enrique Santamaría
Leticia Goni
Eduardo Castellanos
Jose Luis Ibañez Criado
Luis Tercedor
Ignacio García-Bolao
Miguel A. Martínez-González
Jesús Almendral
Miguel Ruiz-Canela
Proteomics and Recurrence of Atrial Fibrillation: A Pilot Study Nested in the PREDIMAR Trial
Lifestyle Genomics
title Proteomics and Recurrence of Atrial Fibrillation: A Pilot Study Nested in the PREDIMAR Trial
title_full Proteomics and Recurrence of Atrial Fibrillation: A Pilot Study Nested in the PREDIMAR Trial
title_fullStr Proteomics and Recurrence of Atrial Fibrillation: A Pilot Study Nested in the PREDIMAR Trial
title_full_unstemmed Proteomics and Recurrence of Atrial Fibrillation: A Pilot Study Nested in the PREDIMAR Trial
title_short Proteomics and Recurrence of Atrial Fibrillation: A Pilot Study Nested in the PREDIMAR Trial
title_sort proteomics and recurrence of atrial fibrillation a pilot study nested in the predimar trial
url https://karger.com/article/doi/10.1159/000543639
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