Investigation of potential Shiga toxin producing Escherichia coli (STEC) associated with a local foodborne outbreak using multidisciplinary approaches
Shiga toxin producing Escherichia coli (STEC) outbreak is a public health concern as it can potentially cause a variety of clinical manifestations including diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). However E. coli are generally innocuous commensal organisms, and there is a...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2016-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221345301630091X |
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| author | Kristen A. Lozinak Niketa Jani Jayanthi Gangiredla Isha Patel Christopher A. Elkins Zonglin Hu Prince A. Kassim Robert A. Myers Pongpan Laksanalamai |
| author_facet | Kristen A. Lozinak Niketa Jani Jayanthi Gangiredla Isha Patel Christopher A. Elkins Zonglin Hu Prince A. Kassim Robert A. Myers Pongpan Laksanalamai |
| author_sort | Kristen A. Lozinak |
| collection | DOAJ |
| description | Shiga toxin producing Escherichia coli (STEC) outbreak is a public health concern as it can potentially cause a variety of clinical manifestations including diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). However E. coli are generally innocuous commensal organisms, and there is a need to discriminate pathogenic from non-pathogenic isolates rapidly and accurately. In this study, we have used standard culture based methods and advanced molecular approaches to characterize E. coli in food in a local outbreak investigation. We show that the application of DNA based detection methods including real-time PCR and DNA microarray along with a traditional culture method can identify the organism implicated in an outbreak at the strain level for pathogenic potential. |
| format | Article |
| id | doaj-art-8b9880d89e7e4f508499bb54bb07a92a |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2016-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-8b9880d89e7e4f508499bb54bb07a92a2025-02-03T02:38:52ZengTsinghua University PressFood Science and Human Wellness2213-45302016-09-015316316810.1016/j.fshw.2016.07.002Investigation of potential Shiga toxin producing Escherichia coli (STEC) associated with a local foodborne outbreak using multidisciplinary approachesKristen A. Lozinak0Niketa Jani1Jayanthi Gangiredla2Isha Patel3Christopher A. Elkins4Zonglin Hu5Prince A. Kassim6Robert A. Myers7Pongpan Laksanalamai8Laboratories Administration, Maryland Department of Health and Mental Hygiene, 1770 Ashland Ave., Baltimore, MD 21205, United StatesLaboratories Administration, Maryland Department of Health and Mental Hygiene, 1770 Ashland Ave., Baltimore, MD 21205, United StatesOffice of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, 8301 Muirkirk Rd., Laurel, MD 20708, United StatesOffice of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, 8301 Muirkirk Rd., Laurel, MD 20708, United StatesOffice of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, 8301 Muirkirk Rd., Laurel, MD 20708, United StatesWinchester Engineering and Analytical Center, Office of Regulatory Affairs, US Food and Drug Administration, 109 Holton St., Winchester, MA 01890, United StatesLaboratories Administration, Maryland Department of Health and Mental Hygiene, 1770 Ashland Ave., Baltimore, MD 21205, United StatesLaboratories Administration, Maryland Department of Health and Mental Hygiene, 1770 Ashland Ave., Baltimore, MD 21205, United StatesLaboratories Administration, Maryland Department of Health and Mental Hygiene, 1770 Ashland Ave., Baltimore, MD 21205, United StatesShiga toxin producing Escherichia coli (STEC) outbreak is a public health concern as it can potentially cause a variety of clinical manifestations including diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). However E. coli are generally innocuous commensal organisms, and there is a need to discriminate pathogenic from non-pathogenic isolates rapidly and accurately. In this study, we have used standard culture based methods and advanced molecular approaches to characterize E. coli in food in a local outbreak investigation. We show that the application of DNA based detection methods including real-time PCR and DNA microarray along with a traditional culture method can identify the organism implicated in an outbreak at the strain level for pathogenic potential.http://www.sciencedirect.com/science/article/pii/S221345301630091XE. coli non-O157Shiga toxin producing E. coli (STEC)RT-PCRDNA microarray |
| spellingShingle | Kristen A. Lozinak Niketa Jani Jayanthi Gangiredla Isha Patel Christopher A. Elkins Zonglin Hu Prince A. Kassim Robert A. Myers Pongpan Laksanalamai Investigation of potential Shiga toxin producing Escherichia coli (STEC) associated with a local foodborne outbreak using multidisciplinary approaches Food Science and Human Wellness E. coli non-O157 Shiga toxin producing E. coli (STEC) RT-PCR DNA microarray |
| title | Investigation of potential Shiga toxin producing Escherichia coli (STEC) associated with a local foodborne outbreak using multidisciplinary approaches |
| title_full | Investigation of potential Shiga toxin producing Escherichia coli (STEC) associated with a local foodborne outbreak using multidisciplinary approaches |
| title_fullStr | Investigation of potential Shiga toxin producing Escherichia coli (STEC) associated with a local foodborne outbreak using multidisciplinary approaches |
| title_full_unstemmed | Investigation of potential Shiga toxin producing Escherichia coli (STEC) associated with a local foodborne outbreak using multidisciplinary approaches |
| title_short | Investigation of potential Shiga toxin producing Escherichia coli (STEC) associated with a local foodborne outbreak using multidisciplinary approaches |
| title_sort | investigation of potential shiga toxin producing escherichia coli stec associated with a local foodborne outbreak using multidisciplinary approaches |
| topic | E. coli non-O157 Shiga toxin producing E. coli (STEC) RT-PCR DNA microarray |
| url | http://www.sciencedirect.com/science/article/pii/S221345301630091X |
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