Structure–activity relationship of 18F-labeled PD-L1-targeting small molecule ligands: impact of radiolabeling strategy on affinity and in vivo performance
Abstract Background Immune checkpoint inhibitor therapy addressing the PD-1/PD-L1 axis is a promising approach in cancer treatment. A clinically suitable radiotracer would allow molecular imaging of the temporospatial changes in tumor PD-L1 expression. This could enable the clinicians to select elig...
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SpringerOpen
2025-07-01
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| Series: | EJNMMI Radiopharmacy and Chemistry |
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| Online Access: | https://doi.org/10.1186/s41181-025-00359-2 |
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| author | Fabian Krutzek Cornelius K. Donat Sven Stadlbauer |
| author_facet | Fabian Krutzek Cornelius K. Donat Sven Stadlbauer |
| author_sort | Fabian Krutzek |
| collection | DOAJ |
| description | Abstract Background Immune checkpoint inhibitor therapy addressing the PD-1/PD-L1 axis is a promising approach in cancer treatment. A clinically suitable radiotracer would allow molecular imaging of the temporospatial changes in tumor PD-L1 expression. This could enable the clinicians to select eligible patients for checkpoint therapy and monitor therapeutic efficacy. Results Four biphenyl-based small-molecule PD-L1 ligands were synthesized using a convergent synthetic route, with a linear sequence of up to eleven steps. Two candidates were covalently labeled with 18F via either an azido glycosyl or PEG2 moiety, while the other two were modified with a RESCA chelator for Al[18F]F2+-labeling. The lipophilicity was assessed through determination of log D 7.4 values. In vitro binding affinities (inhibition constant, K i) toward PD-L1 were determined in competition with one of our previously published biphenyl-based small-molecule (K D = ~ 21 nM). Compared to this compound, both covalently labeled 18F-ligands exhibited decreased water solubility (log D 7.4 ~ − 2.5 and − 2.7), along with a markedly reduced (K i = 200‒500 nM) affinity. This was in line with in vivo small animal PET, where both compounds were characterized by a negligible tumor uptake, lack of contrast between target-positive/negative tumors and exclusively unfavorable hepatobiliary excretion. Similar results were observed for the chelator-modified ligands with slightly increased hydrophilicity (log D 7.4 ~ − 2.8 and − 2.9), showing a binding affinity of 150 nM for one compound, while binding was lost completely for the other. Again, a poor in vivo performance was observed, characterized by hepatobiliary clearance and lack of specific tumor uptake in the PD-L1 positive tumor. Conclusion Four biphenyl-based, 18F-labeled PD-L1 radioligands were developed using prosthetic groups (azido glycosyl or PEG2) for covalent fluorination and Al[18F]F2⁺-complexation with the RESCA chelator. Despite limited in vitro and in vivo performance, these fluorination approaches offer a foundation for developing improved PD-L1 radioligands after increasing the hydrophilicity and the spacing between the radiolabel and binding motif. |
| format | Article |
| id | doaj-art-8b92cb8c6e5d4ff59cf0c32f478bca18 |
| institution | Kabale University |
| issn | 2365-421X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | EJNMMI Radiopharmacy and Chemistry |
| spelling | doaj-art-8b92cb8c6e5d4ff59cf0c32f478bca182025-08-20T03:42:03ZengSpringerOpenEJNMMI Radiopharmacy and Chemistry2365-421X2025-07-0110113510.1186/s41181-025-00359-2Structure–activity relationship of 18F-labeled PD-L1-targeting small molecule ligands: impact of radiolabeling strategy on affinity and in vivo performanceFabian Krutzek0Cornelius K. Donat1Sven Stadlbauer2Helmholtz-Zentrum Dresden-Rossendorf, Institute of Radiopharmaceutical Cancer ResearchHelmholtz-Zentrum Dresden-Rossendorf, Institute of Radiopharmaceutical Cancer ResearchHelmholtz-Zentrum Dresden-Rossendorf, Institute of Radiopharmaceutical Cancer ResearchAbstract Background Immune checkpoint inhibitor therapy addressing the PD-1/PD-L1 axis is a promising approach in cancer treatment. A clinically suitable radiotracer would allow molecular imaging of the temporospatial changes in tumor PD-L1 expression. This could enable the clinicians to select eligible patients for checkpoint therapy and monitor therapeutic efficacy. Results Four biphenyl-based small-molecule PD-L1 ligands were synthesized using a convergent synthetic route, with a linear sequence of up to eleven steps. Two candidates were covalently labeled with 18F via either an azido glycosyl or PEG2 moiety, while the other two were modified with a RESCA chelator for Al[18F]F2+-labeling. The lipophilicity was assessed through determination of log D 7.4 values. In vitro binding affinities (inhibition constant, K i) toward PD-L1 were determined in competition with one of our previously published biphenyl-based small-molecule (K D = ~ 21 nM). Compared to this compound, both covalently labeled 18F-ligands exhibited decreased water solubility (log D 7.4 ~ − 2.5 and − 2.7), along with a markedly reduced (K i = 200‒500 nM) affinity. This was in line with in vivo small animal PET, where both compounds were characterized by a negligible tumor uptake, lack of contrast between target-positive/negative tumors and exclusively unfavorable hepatobiliary excretion. Similar results were observed for the chelator-modified ligands with slightly increased hydrophilicity (log D 7.4 ~ − 2.8 and − 2.9), showing a binding affinity of 150 nM for one compound, while binding was lost completely for the other. Again, a poor in vivo performance was observed, characterized by hepatobiliary clearance and lack of specific tumor uptake in the PD-L1 positive tumor. Conclusion Four biphenyl-based, 18F-labeled PD-L1 radioligands were developed using prosthetic groups (azido glycosyl or PEG2) for covalent fluorination and Al[18F]F2⁺-complexation with the RESCA chelator. Despite limited in vitro and in vivo performance, these fluorination approaches offer a foundation for developing improved PD-L1 radioligands after increasing the hydrophilicity and the spacing between the radiolabel and binding motif.https://doi.org/10.1186/s41181-025-00359-2PD-L1RadioligandsPositron emission tomographySmall moleculesFluorine-18Chelators |
| spellingShingle | Fabian Krutzek Cornelius K. Donat Sven Stadlbauer Structure–activity relationship of 18F-labeled PD-L1-targeting small molecule ligands: impact of radiolabeling strategy on affinity and in vivo performance EJNMMI Radiopharmacy and Chemistry PD-L1 Radioligands Positron emission tomography Small molecules Fluorine-18 Chelators |
| title | Structure–activity relationship of 18F-labeled PD-L1-targeting small molecule ligands: impact of radiolabeling strategy on affinity and in vivo performance |
| title_full | Structure–activity relationship of 18F-labeled PD-L1-targeting small molecule ligands: impact of radiolabeling strategy on affinity and in vivo performance |
| title_fullStr | Structure–activity relationship of 18F-labeled PD-L1-targeting small molecule ligands: impact of radiolabeling strategy on affinity and in vivo performance |
| title_full_unstemmed | Structure–activity relationship of 18F-labeled PD-L1-targeting small molecule ligands: impact of radiolabeling strategy on affinity and in vivo performance |
| title_short | Structure–activity relationship of 18F-labeled PD-L1-targeting small molecule ligands: impact of radiolabeling strategy on affinity and in vivo performance |
| title_sort | structure activity relationship of 18f labeled pd l1 targeting small molecule ligands impact of radiolabeling strategy on affinity and in vivo performance |
| topic | PD-L1 Radioligands Positron emission tomography Small molecules Fluorine-18 Chelators |
| url | https://doi.org/10.1186/s41181-025-00359-2 |
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