Antioxidant Capacity and Phenolic Profile of Defatted Seed Powder Derived from Organically Grown Vine Crops
Grape seeds derived from winemaking pomace represent a significant waste stream with potential for valorization due to their rich phenolic content. The present study aimed to assess the phenolic composition and antioxidant potency of defatted grape seeds powder (DP) resulting after oil extraction t...
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AcademicPres
2024-11-01
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| Series: | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology |
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| Online Access: | https://journals.usamvcluj.ro/index.php/fst/article/view/14985 |
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| author | Daniela GITEA Manuel Alexandru GITEA Andrei-Flavius RADU Manuela Bianca PASCA Simona Gabriela BUNGAU Delia Mirela TIT |
| author_facet | Daniela GITEA Manuel Alexandru GITEA Andrei-Flavius RADU Manuela Bianca PASCA Simona Gabriela BUNGAU Delia Mirela TIT |
| author_sort | Daniela GITEA |
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Grape seeds derived from winemaking pomace represent a significant waste stream with potential for valorization due to their rich phenolic content. The present study aimed to assess the phenolic composition and antioxidant potency of defatted grape seeds powder (DP) resulting after oil extraction through three different methods (i.e., cold stirring (ST), ultrasound-assisted extraction (US), and microwave-assisted extraction (MW)) from two grape varieties (i.e., Fetească Regală and Merlot) grown under organic farming conditions. The phenolic profile was determined using High-performance liquid chromatography with diode array detection and mass spectrometry operating in electrospray ionization positive mode, while the total phenolic content was determined using an adapted Folin-Ciocalteu technique, The samples' antioxidant properties were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability assessments, as well as Cupric ion reducing antioxidant capacity and Ferric reducing antioxidant power assays. The phenolic qualitative profile differed between the two varieties and was unaffected by the defatting method. Instead, at the level of each variety, significant variations (p<0.05) were observed in the quantity of phenolic compounds, depending on the defatting method. Moreover, the total phenolic content was significantly higher in the MW-DP compared to ST-DP and US-DP (p<0.05), particularly from Fetească Regală grape seeds. The DPPH assay demonstrated the highest antioxidant activity in MW-DP from Fetească Regală. The study underscores the importance of the extraction methods and grape variety on the phenolic profile and antioxidant potency of DP. These findings suggest potential applications in pharmaceuticals, functional foods, and cosmetics, offering sustainable solutions for grape pomace utilization.
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| format | Article |
| id | doaj-art-8b8f01f6191243ecb77e007d7e99a2e8 |
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| issn | 2344-2344 2344-5300 |
| language | English |
| publishDate | 2024-11-01 |
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| series | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology |
| spelling | doaj-art-8b8f01f6191243ecb77e007d7e99a2e82025-08-20T01:54:25ZengAcademicPresBulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology2344-23442344-53002024-11-0181210.15835/buasvmcn-fst:2024.000914985Antioxidant Capacity and Phenolic Profile of Defatted Seed Powder Derived from Organically Grown Vine CropsDaniela GITEA0Manuel Alexandru GITEA1Andrei-Flavius RADU2Manuela Bianca PASCA3Simona Gabriela BUNGAU4Delia Mirela TIT5Faculty of Medicine and Pharmacy, University of Oradea, 410028 Oradea, RomaniaFaculty of Environmental Protection, University of Oradea, 410087, Oradea, RomaniaFaculty of Medicine and Pharmacy, University of Oradea, 410028 Oradea, Romania; Doctoral School of Biomedical Sciences, University of Oradea, 410087 Oradea, Romania Faculty of Medicine and Pharmacy, University of Oradea, 410028 Oradea, RomaniaFaculty of Medicine and Pharmacy, University of Oradea, 410028 Oradea, Romania; Doctoral School of Biomedical Sciences, University of Oradea, 410087 Oradea, RomaniaFaculty of Medicine and Pharmacy, University of Oradea, 410028 Oradea, Romania; Doctoral School of Biomedical Sciences, University of Oradea, 410087 Oradea, Romania Grape seeds derived from winemaking pomace represent a significant waste stream with potential for valorization due to their rich phenolic content. The present study aimed to assess the phenolic composition and antioxidant potency of defatted grape seeds powder (DP) resulting after oil extraction through three different methods (i.e., cold stirring (ST), ultrasound-assisted extraction (US), and microwave-assisted extraction (MW)) from two grape varieties (i.e., Fetească Regală and Merlot) grown under organic farming conditions. The phenolic profile was determined using High-performance liquid chromatography with diode array detection and mass spectrometry operating in electrospray ionization positive mode, while the total phenolic content was determined using an adapted Folin-Ciocalteu technique, The samples' antioxidant properties were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability assessments, as well as Cupric ion reducing antioxidant capacity and Ferric reducing antioxidant power assays. The phenolic qualitative profile differed between the two varieties and was unaffected by the defatting method. Instead, at the level of each variety, significant variations (p<0.05) were observed in the quantity of phenolic compounds, depending on the defatting method. Moreover, the total phenolic content was significantly higher in the MW-DP compared to ST-DP and US-DP (p<0.05), particularly from Fetească Regală grape seeds. The DPPH assay demonstrated the highest antioxidant activity in MW-DP from Fetească Regală. The study underscores the importance of the extraction methods and grape variety on the phenolic profile and antioxidant potency of DP. These findings suggest potential applications in pharmaceuticals, functional foods, and cosmetics, offering sustainable solutions for grape pomace utilization. https://journals.usamvcluj.ro/index.php/fst/article/view/14985Grape seeds powder; phenolic composition; antioxidant potency; extraction methods. |
| spellingShingle | Daniela GITEA Manuel Alexandru GITEA Andrei-Flavius RADU Manuela Bianca PASCA Simona Gabriela BUNGAU Delia Mirela TIT Antioxidant Capacity and Phenolic Profile of Defatted Seed Powder Derived from Organically Grown Vine Crops Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology Grape seeds powder; phenolic composition; antioxidant potency; extraction methods. |
| title | Antioxidant Capacity and Phenolic Profile of Defatted Seed Powder Derived from Organically Grown Vine Crops |
| title_full | Antioxidant Capacity and Phenolic Profile of Defatted Seed Powder Derived from Organically Grown Vine Crops |
| title_fullStr | Antioxidant Capacity and Phenolic Profile of Defatted Seed Powder Derived from Organically Grown Vine Crops |
| title_full_unstemmed | Antioxidant Capacity and Phenolic Profile of Defatted Seed Powder Derived from Organically Grown Vine Crops |
| title_short | Antioxidant Capacity and Phenolic Profile of Defatted Seed Powder Derived from Organically Grown Vine Crops |
| title_sort | antioxidant capacity and phenolic profile of defatted seed powder derived from organically grown vine crops |
| topic | Grape seeds powder; phenolic composition; antioxidant potency; extraction methods. |
| url | https://journals.usamvcluj.ro/index.php/fst/article/view/14985 |
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