Analytical Characteristics and Clinical Performance of Anti-Müllerian Hormone Immunoassay on the ADVIA Centaur<sup>®</sup> System: A Comparison with Other Chemiluminescent Methods
Background: Serum anti-Müllerian hormone (AMH) levels and antral follicle count are key in evaluating ovarian reserve (OR) for fertility. The performance of the Siemens Healthineers AMH assay was assessed on the ADVIA Centaur<sup>®</sup> System. Methods: Analytical characteristics, clini...
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MDPI AG
2024-11-01
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| Series: | Endocrines |
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| Online Access: | https://www.mdpi.com/2673-396X/5/4/37 |
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| author | Jelena Bogdanovic Kaitlin Freeman Chadwick Brown Rachel Singleton Millie Behera Jeanne E. O’Brien Edward Zbella Robert H. Christenson |
| author_facet | Jelena Bogdanovic Kaitlin Freeman Chadwick Brown Rachel Singleton Millie Behera Jeanne E. O’Brien Edward Zbella Robert H. Christenson |
| author_sort | Jelena Bogdanovic |
| collection | DOAJ |
| description | Background: Serum anti-Müllerian hormone (AMH) levels and antral follicle count are key in evaluating ovarian reserve (OR) for fertility. The performance of the Siemens Healthineers AMH assay was assessed on the ADVIA Centaur<sup>®</sup> System. Methods: Analytical characteristics, clinical performance, and method comparison studies were performed in a prospective cohort of 532 women at fertility clinics. Serum AMH levels were determined using ADVIA Centaur, Beckman Access<sup>®</sup>, and Roche Elecsys<sup>®</sup> assays. Results: The limit of quantitation for the ADVIA Centaur AMH assay was 0.030 ng/mL. Repeatability was ≤2.9% CV, within-lab repeatability was ≤3.2% CV, and reproducibility was ≤4.4% CV. Results using serum or lithium heparin sample types were equivalent. Diagnostic sensitivity across assays ranged from 77.3% to 90.2% and specificity ranged from 51.0 to 71.0%; corresponding positive and negative predictive values ranged from 66.6% to 74.3% and 74.2% to 83.0%, respectively. Receiver operating characteristic analyses demonstrated that the assays have a high probability for discriminating between diminished–normal and high OR. ADVIA and Beckman assays agreed according to ADVIA = 1.00 × Beckman + 0.014 ng/mL, τ = 0.909, while a more modest correlation of ADVIA = 1.41 × Roche − 0.024 ng/mL, τ = 0.777 was observed with Roche assay. Conclusions: The ADVIA Centaur assay demonstrates acceptable analytical characteristics and clinical performance comparable to the Roche AMH assay and is essentially interchangeable with the Beckman AMH assay for reliable OR assessment. |
| format | Article |
| id | doaj-art-8b8ac72ed32f4eaa8c8fef2b17077a1f |
| institution | OA Journals |
| issn | 2673-396X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Endocrines |
| spelling | doaj-art-8b8ac72ed32f4eaa8c8fef2b17077a1f2025-08-20T02:00:37ZengMDPI AGEndocrines2673-396X2024-11-015451652810.3390/endocrines5040037Analytical Characteristics and Clinical Performance of Anti-Müllerian Hormone Immunoassay on the ADVIA Centaur<sup>®</sup> System: A Comparison with Other Chemiluminescent MethodsJelena Bogdanovic0Kaitlin Freeman1Chadwick Brown2Rachel Singleton3Millie Behera4Jeanne E. O’Brien5Edward Zbella6Robert H. Christenson7Siemens Healthineers, Tarrytown, NY 10591, USASiemens Healthineers, Tarrytown, NY 10591, USASiemens Healthineers, Tarrytown, NY 10591, USASiemens Healthineers, Tarrytown, NY 10591, USABloom Reproductive Institute, Scottsdale, AZ 85258, USAShady Grove Fertility Center, Rockville, MD 20850, USAWomen’s Medical Research Group, LLC, Clearwater, FL 33759, USAClinical Chemistry Research Lab, University of Maryland, Baltimore, MD 21201, USABackground: Serum anti-Müllerian hormone (AMH) levels and antral follicle count are key in evaluating ovarian reserve (OR) for fertility. The performance of the Siemens Healthineers AMH assay was assessed on the ADVIA Centaur<sup>®</sup> System. Methods: Analytical characteristics, clinical performance, and method comparison studies were performed in a prospective cohort of 532 women at fertility clinics. Serum AMH levels were determined using ADVIA Centaur, Beckman Access<sup>®</sup>, and Roche Elecsys<sup>®</sup> assays. Results: The limit of quantitation for the ADVIA Centaur AMH assay was 0.030 ng/mL. Repeatability was ≤2.9% CV, within-lab repeatability was ≤3.2% CV, and reproducibility was ≤4.4% CV. Results using serum or lithium heparin sample types were equivalent. Diagnostic sensitivity across assays ranged from 77.3% to 90.2% and specificity ranged from 51.0 to 71.0%; corresponding positive and negative predictive values ranged from 66.6% to 74.3% and 74.2% to 83.0%, respectively. Receiver operating characteristic analyses demonstrated that the assays have a high probability for discriminating between diminished–normal and high OR. ADVIA and Beckman assays agreed according to ADVIA = 1.00 × Beckman + 0.014 ng/mL, τ = 0.909, while a more modest correlation of ADVIA = 1.41 × Roche − 0.024 ng/mL, τ = 0.777 was observed with Roche assay. Conclusions: The ADVIA Centaur assay demonstrates acceptable analytical characteristics and clinical performance comparable to the Roche AMH assay and is essentially interchangeable with the Beckman AMH assay for reliable OR assessment.https://www.mdpi.com/2673-396X/5/4/37anti-Müllerian hormoneantral follicle countovarian reserveADVIA Centaurautomated analyzerprospective studies |
| spellingShingle | Jelena Bogdanovic Kaitlin Freeman Chadwick Brown Rachel Singleton Millie Behera Jeanne E. O’Brien Edward Zbella Robert H. Christenson Analytical Characteristics and Clinical Performance of Anti-Müllerian Hormone Immunoassay on the ADVIA Centaur<sup>®</sup> System: A Comparison with Other Chemiluminescent Methods Endocrines anti-Müllerian hormone antral follicle count ovarian reserve ADVIA Centaur automated analyzer prospective studies |
| title | Analytical Characteristics and Clinical Performance of Anti-Müllerian Hormone Immunoassay on the ADVIA Centaur<sup>®</sup> System: A Comparison with Other Chemiluminescent Methods |
| title_full | Analytical Characteristics and Clinical Performance of Anti-Müllerian Hormone Immunoassay on the ADVIA Centaur<sup>®</sup> System: A Comparison with Other Chemiluminescent Methods |
| title_fullStr | Analytical Characteristics and Clinical Performance of Anti-Müllerian Hormone Immunoassay on the ADVIA Centaur<sup>®</sup> System: A Comparison with Other Chemiluminescent Methods |
| title_full_unstemmed | Analytical Characteristics and Clinical Performance of Anti-Müllerian Hormone Immunoassay on the ADVIA Centaur<sup>®</sup> System: A Comparison with Other Chemiluminescent Methods |
| title_short | Analytical Characteristics and Clinical Performance of Anti-Müllerian Hormone Immunoassay on the ADVIA Centaur<sup>®</sup> System: A Comparison with Other Chemiluminescent Methods |
| title_sort | analytical characteristics and clinical performance of anti mullerian hormone immunoassay on the advia centaur sup r sup system a comparison with other chemiluminescent methods |
| topic | anti-Müllerian hormone antral follicle count ovarian reserve ADVIA Centaur automated analyzer prospective studies |
| url | https://www.mdpi.com/2673-396X/5/4/37 |
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