Altered dendritic morphology of MEC II pyramidal and stellate cells in Rett syndrome mice
IntroductionMutations in the methyl-CpG-binding protein-2 gene (MECP2), which cause Rett syndrome (RTT), disrupt neuronal activity; however, the impact of the MECP2 loss-of-function on the cytoarchitecture of medial entorhinal cortex layer II (MECII) neurons—crucial for spatial memory and learning—r...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Neuroanatomy |
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| author | Manigandan Krishnan Ayishal B. Mydeen Mohammed M. Nakhal Marwa F. Ibrahim Richard L. Jayaraj Milos R. Ljubisavljevic Mohammad I. K. Hamad Fatima Y. Ismail Fatima Y. Ismail |
| author_facet | Manigandan Krishnan Ayishal B. Mydeen Mohammed M. Nakhal Marwa F. Ibrahim Richard L. Jayaraj Milos R. Ljubisavljevic Mohammad I. K. Hamad Fatima Y. Ismail Fatima Y. Ismail |
| author_sort | Manigandan Krishnan |
| collection | DOAJ |
| description | IntroductionMutations in the methyl-CpG-binding protein-2 gene (MECP2), which cause Rett syndrome (RTT), disrupt neuronal activity; however, the impact of the MECP2 loss-of-function on the cytoarchitecture of medial entorhinal cortex layer II (MECII) neurons—crucial for spatial memory and learning—remains poorly understood.MethodsIn this study, we utilized Golgi staining and neuron tracing in the Mecp2+/− mouse model of RTT to investigate the pyramidal and stellate cell alterations in MECII.Results and discussionOur findings revealed that pyramidal cells displayed a significant reduction in apical dendritic length, soma size, and spine density, while basal dendrites showed increased dendritic complexity and branching. On the other hand, stellate cells exhibited dendritic hypertrophy along with increased soma size, primary dendrites, and localized increase in dendritic intersections, despite an overall reduction in total dendritic length and spine density. These findings underscore the notion that MECP2 loss-of-function can disrupt MECII pyramidal and stellate cell cytoarchitecture in a cell-type-specific manner, emphasizing its critical role in maintaining proper dendritic morphology in circuits, which is crucial for learning and memory. |
| format | Article |
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| institution | Kabale University |
| issn | 1662-5129 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Neuroanatomy |
| spelling | doaj-art-8b7be3d9fa184c0a97d50e6d201010c72025-08-20T03:32:09ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292025-06-011910.3389/fnana.2025.15804351580435Altered dendritic morphology of MEC II pyramidal and stellate cells in Rett syndrome miceManigandan Krishnan0Ayishal B. Mydeen1Mohammed M. Nakhal2Marwa F. Ibrahim3Richard L. Jayaraj4Milos R. Ljubisavljevic5Mohammad I. K. Hamad6Fatima Y. Ismail7Fatima Y. Ismail8Department of Pediatrics, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Pediatrics, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Pediatrics, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Neurology (Adjunct), Johns Hopkins University School of Medicine, Baltimore, MD, United StatesIntroductionMutations in the methyl-CpG-binding protein-2 gene (MECP2), which cause Rett syndrome (RTT), disrupt neuronal activity; however, the impact of the MECP2 loss-of-function on the cytoarchitecture of medial entorhinal cortex layer II (MECII) neurons—crucial for spatial memory and learning—remains poorly understood.MethodsIn this study, we utilized Golgi staining and neuron tracing in the Mecp2+/− mouse model of RTT to investigate the pyramidal and stellate cell alterations in MECII.Results and discussionOur findings revealed that pyramidal cells displayed a significant reduction in apical dendritic length, soma size, and spine density, while basal dendrites showed increased dendritic complexity and branching. On the other hand, stellate cells exhibited dendritic hypertrophy along with increased soma size, primary dendrites, and localized increase in dendritic intersections, despite an overall reduction in total dendritic length and spine density. These findings underscore the notion that MECP2 loss-of-function can disrupt MECII pyramidal and stellate cell cytoarchitecture in a cell-type-specific manner, emphasizing its critical role in maintaining proper dendritic morphology in circuits, which is crucial for learning and memory.https://www.frontiersin.org/articles/10.3389/fnana.2025.1580435/fullRett syndromeMecp2 mouse modeldendritic branchingpyramidal cellsstellate cellsmedial entorhinal cortex |
| spellingShingle | Manigandan Krishnan Ayishal B. Mydeen Mohammed M. Nakhal Marwa F. Ibrahim Richard L. Jayaraj Milos R. Ljubisavljevic Mohammad I. K. Hamad Fatima Y. Ismail Fatima Y. Ismail Altered dendritic morphology of MEC II pyramidal and stellate cells in Rett syndrome mice Frontiers in Neuroanatomy Rett syndrome Mecp2 mouse model dendritic branching pyramidal cells stellate cells medial entorhinal cortex |
| title | Altered dendritic morphology of MEC II pyramidal and stellate cells in Rett syndrome mice |
| title_full | Altered dendritic morphology of MEC II pyramidal and stellate cells in Rett syndrome mice |
| title_fullStr | Altered dendritic morphology of MEC II pyramidal and stellate cells in Rett syndrome mice |
| title_full_unstemmed | Altered dendritic morphology of MEC II pyramidal and stellate cells in Rett syndrome mice |
| title_short | Altered dendritic morphology of MEC II pyramidal and stellate cells in Rett syndrome mice |
| title_sort | altered dendritic morphology of mec ii pyramidal and stellate cells in rett syndrome mice |
| topic | Rett syndrome Mecp2 mouse model dendritic branching pyramidal cells stellate cells medial entorhinal cortex |
| url | https://www.frontiersin.org/articles/10.3389/fnana.2025.1580435/full |
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