ANTIPROLIFERATIVE EFFECT OF LACTOBACILLUS PLANTARUM L4 STRAIN ISOLATED FROM CERVICOVAGINAL MICROFLORA ON HELA CANCER CELL LINE
Objective: Lactobacillus has been shown to inhibit proliferation of various cancer cells, but the effects of vaginal Lactobacillus on cervical cancer cells have rarely been reported. The goal of this investigation was to assess the anti-proliferative effect on cancer cell line HeLa (Human Cervical C...
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Istanbul University Press
2022-01-01
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| Series: | İstanbul Tıp Fakültesi Dergisi |
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| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/E7CD5990B92149D39004C5487C2D552F |
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| author | Esin Kıray Serap Yalçın Ergin Kariptaş |
| author_facet | Esin Kıray Serap Yalçın Ergin Kariptaş |
| author_sort | Esin Kıray |
| collection | DOAJ |
| description | Objective: Lactobacillus has been shown to inhibit proliferation of various cancer cells, but the effects of vaginal Lactobacillus on cervical cancer cells have rarely been reported. The goal of this investigation was to assess the anti-proliferative effect on cancer cell line HeLa (Human Cervical Carcinoma Cell) and potential probiotic properties of Lactobacillus plantarum L4 isolated from cervicovaginal flora of healthy women in Turkey. Materials and Methods: Molecular identification of the species was performed by 16S rDNA analysis. Probiotic properties of the L4 strain were investigated by conventional methods. Human Interleukin-10 (IL-10) and Tumor Necrosis Factor- alpha (TNF-alpha) ELISA kits were used in the evelation of the immune modulator effect of the L4 strain. The antiproliferative effect of the L4 strain on the HeLA cell line was performed using the XTT kit. Results: L. plantarum L4 strain exhibited strong probiotic properties. The L4 strain showed an anti-inflammatory effect on HeLa by reducing the production of TNF-α and increased IL-10 production. The greatest antiproliferative effect of L. plantarum L4 strain on HeLa cells was observed at the highest dose of the metabolite 0.0006 gr/ml with a death rate of 90-95% while the number of living cells was found to be between 5-10%. The strain showed no anticancer effect on human umbilical vein endothelial cells (HUVEC). Conclusion: L. plantarum L4 strain, with strong probiotic properties, can be considered a promising treatment candidate for HPV cancer due to its immunomodulatory effect and high antiproliferative effect, even in very small doses. |
| format | Article |
| id | doaj-art-8b7a653fbc1b491793eabfc91ac78a50 |
| institution | OA Journals |
| issn | 1305-6441 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | İstanbul Tıp Fakültesi Dergisi |
| spelling | doaj-art-8b7a653fbc1b491793eabfc91ac78a502025-08-20T02:15:19ZengIstanbul University Pressİstanbul Tıp Fakültesi Dergisi1305-64412022-01-01851677610.26650/IUITFD.915299123456ANTIPROLIFERATIVE EFFECT OF LACTOBACILLUS PLANTARUM L4 STRAIN ISOLATED FROM CERVICOVAGINAL MICROFLORA ON HELA CANCER CELL LINEEsin Kıray0https://orcid.org/0000-0002-6908-5909Serap Yalçın1https://orcid.org/0000-0002-9584-266XErgin Kariptaş2https://orcid.org/0000-0001-6513-9589Kırşehir Ahi Evran Üniversitesi, Kirsehir, TurkiyeKırşehir Ahi Evran Üniversitesi, Kirsehir, TurkiyeOndokuz Mayıs Üniversitesi, Samsun, TurkiyeObjective: Lactobacillus has been shown to inhibit proliferation of various cancer cells, but the effects of vaginal Lactobacillus on cervical cancer cells have rarely been reported. The goal of this investigation was to assess the anti-proliferative effect on cancer cell line HeLa (Human Cervical Carcinoma Cell) and potential probiotic properties of Lactobacillus plantarum L4 isolated from cervicovaginal flora of healthy women in Turkey. Materials and Methods: Molecular identification of the species was performed by 16S rDNA analysis. Probiotic properties of the L4 strain were investigated by conventional methods. Human Interleukin-10 (IL-10) and Tumor Necrosis Factor- alpha (TNF-alpha) ELISA kits were used in the evelation of the immune modulator effect of the L4 strain. The antiproliferative effect of the L4 strain on the HeLA cell line was performed using the XTT kit. Results: L. plantarum L4 strain exhibited strong probiotic properties. The L4 strain showed an anti-inflammatory effect on HeLa by reducing the production of TNF-α and increased IL-10 production. The greatest antiproliferative effect of L. plantarum L4 strain on HeLa cells was observed at the highest dose of the metabolite 0.0006 gr/ml with a death rate of 90-95% while the number of living cells was found to be between 5-10%. The strain showed no anticancer effect on human umbilical vein endothelial cells (HUVEC). Conclusion: L. plantarum L4 strain, with strong probiotic properties, can be considered a promising treatment candidate for HPV cancer due to its immunomodulatory effect and high antiproliferative effect, even in very small doses.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/E7CD5990B92149D39004C5487C2D552Flactobacillus plantarumantiproliferative effecthelail-10tnf-α |
| spellingShingle | Esin Kıray Serap Yalçın Ergin Kariptaş ANTIPROLIFERATIVE EFFECT OF LACTOBACILLUS PLANTARUM L4 STRAIN ISOLATED FROM CERVICOVAGINAL MICROFLORA ON HELA CANCER CELL LINE İstanbul Tıp Fakültesi Dergisi lactobacillus plantarum antiproliferative effect hela il-10 tnf-α |
| title | ANTIPROLIFERATIVE EFFECT OF LACTOBACILLUS PLANTARUM L4 STRAIN ISOLATED FROM CERVICOVAGINAL MICROFLORA ON HELA CANCER CELL LINE |
| title_full | ANTIPROLIFERATIVE EFFECT OF LACTOBACILLUS PLANTARUM L4 STRAIN ISOLATED FROM CERVICOVAGINAL MICROFLORA ON HELA CANCER CELL LINE |
| title_fullStr | ANTIPROLIFERATIVE EFFECT OF LACTOBACILLUS PLANTARUM L4 STRAIN ISOLATED FROM CERVICOVAGINAL MICROFLORA ON HELA CANCER CELL LINE |
| title_full_unstemmed | ANTIPROLIFERATIVE EFFECT OF LACTOBACILLUS PLANTARUM L4 STRAIN ISOLATED FROM CERVICOVAGINAL MICROFLORA ON HELA CANCER CELL LINE |
| title_short | ANTIPROLIFERATIVE EFFECT OF LACTOBACILLUS PLANTARUM L4 STRAIN ISOLATED FROM CERVICOVAGINAL MICROFLORA ON HELA CANCER CELL LINE |
| title_sort | antiproliferative effect of lactobacillus plantarum l4 strain isolated from cervicovaginal microflora on hela cancer cell line |
| topic | lactobacillus plantarum antiproliferative effect hela il-10 tnf-α |
| url | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/E7CD5990B92149D39004C5487C2D552F |
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