Th1/Th2 Dichotomy in Obese Women with Gestational Diabetes and Their Macrosomic Babies
The aim of the study was to assess T cell differentiation and the modulation of inflammatory cytokines in obese and gestational diabetes mellitus (GDM) women and their macrosomic newborns. Hence, immediately after delivery, blood samples were collected through the mother’s arm vein and the umbilical...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2018/8474617 |
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| author | A. Seck A. Hichami S. Doucouré F. Diallo Agne H. Bassène A. Ba C. Sokhna N. A. Khan A. Samb |
| author_facet | A. Seck A. Hichami S. Doucouré F. Diallo Agne H. Bassène A. Ba C. Sokhna N. A. Khan A. Samb |
| author_sort | A. Seck |
| collection | DOAJ |
| description | The aim of the study was to assess T cell differentiation and the modulation of inflammatory cytokines in obese and gestational diabetes mellitus (GDM) women and their macrosomic newborns. Hence, immediately after delivery, blood samples were collected through the mother’s arm vein and the umbilical cordon vein. Biochemical parameters measured were HbA1C, glucose, insulin, triglyceride (TG), total cholesterol (Tchol), HDL cholesterol (HDLchol), and LDL cholesterol (LDLchol). T lymphocytes were purified from the total blood with Ficoll-Paque. The mRNA expression of inflammatory markers in T cells was determined by RT-qPCR. We observed that diabetic mothers exhibited higher HbA1C, glycemia, insulinemia, TG, Tchol, HDLchol, and LDLchol levels than control mothers. Glycemia was not significantly different between macrosomic and control newborns. However, insulinemia was high in macrosomic babies. TG, Tchol, HDLchol, and LDLchol were not significantly different between macrosomic and control babies. In diabetic mothers, mRNA expression of the Th1 cell subtype was significantly increased. Th1 markers were upregulated in babies born to diabetic women than in control newborns. However, expression of two Th2 markers (GATA3 and IL-4) was not significantly different between control and GDM women and between their respective newborns. Interestingly, IL-10 mRNA expression was decreased in diabetic mothers and their offsprings. The Th1/Th2 cytokine ratio was increased in GDM obese mothers and their macrosomic newborns, suggesting a proinflammatory status in these subjects. |
| format | Article |
| id | doaj-art-8b6724848087452f912553467072af46 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-8b6724848087452f912553467072af462025-02-03T06:11:01ZengWileyJournal of Diabetes Research2314-67452314-67532018-01-01201810.1155/2018/84746178474617Th1/Th2 Dichotomy in Obese Women with Gestational Diabetes and Their Macrosomic BabiesA. Seck0A. Hichami1S. Doucouré2F. Diallo Agne3H. Bassène4A. Ba5C. Sokhna6N. A. Khan7A. Samb8Laboratory of Physiology and Functional Explorations, Faculty of Medicine, Pharmacy and Odontology, Cheikh Anta Diop University, 5005 Dakar-Fann, SenegalU1231 INSERM/Université de Bourgogne-Franche Comté (UBFC)/Agro-Sup, Physiologie de la Nutrition & Toxicologie, Dijon 21000, FranceInstitute of Research for Development, VITROME Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, IHU-Mediterranean Infection, CP18524 Dakar, SenegalLaboratory of Biochemistry and Molecular Biology, Faculty of Medicine, Pharmacy and Odontology, Cheikh Anta Diop University, 5005 Dakar-Fann, SenegalInstitute of Research for Development, VITROME Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, IHU-Mediterranean Infection, CP18524 Dakar, SenegalLaboratory of Physiology and Functional Explorations, Faculty of Medicine, Pharmacy and Odontology, Cheikh Anta Diop University, 5005 Dakar-Fann, SenegalInstitute of Research for Development, VITROME Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, IHU-Mediterranean Infection, CP18524 Dakar, SenegalU1231 INSERM/Université de Bourgogne-Franche Comté (UBFC)/Agro-Sup, Physiologie de la Nutrition & Toxicologie, Dijon 21000, FranceLaboratory of Physiology and Functional Explorations, Faculty of Medicine, Pharmacy and Odontology, Cheikh Anta Diop University, 5005 Dakar-Fann, SenegalThe aim of the study was to assess T cell differentiation and the modulation of inflammatory cytokines in obese and gestational diabetes mellitus (GDM) women and their macrosomic newborns. Hence, immediately after delivery, blood samples were collected through the mother’s arm vein and the umbilical cordon vein. Biochemical parameters measured were HbA1C, glucose, insulin, triglyceride (TG), total cholesterol (Tchol), HDL cholesterol (HDLchol), and LDL cholesterol (LDLchol). T lymphocytes were purified from the total blood with Ficoll-Paque. The mRNA expression of inflammatory markers in T cells was determined by RT-qPCR. We observed that diabetic mothers exhibited higher HbA1C, glycemia, insulinemia, TG, Tchol, HDLchol, and LDLchol levels than control mothers. Glycemia was not significantly different between macrosomic and control newborns. However, insulinemia was high in macrosomic babies. TG, Tchol, HDLchol, and LDLchol were not significantly different between macrosomic and control babies. In diabetic mothers, mRNA expression of the Th1 cell subtype was significantly increased. Th1 markers were upregulated in babies born to diabetic women than in control newborns. However, expression of two Th2 markers (GATA3 and IL-4) was not significantly different between control and GDM women and between their respective newborns. Interestingly, IL-10 mRNA expression was decreased in diabetic mothers and their offsprings. The Th1/Th2 cytokine ratio was increased in GDM obese mothers and their macrosomic newborns, suggesting a proinflammatory status in these subjects.http://dx.doi.org/10.1155/2018/8474617 |
| spellingShingle | A. Seck A. Hichami S. Doucouré F. Diallo Agne H. Bassène A. Ba C. Sokhna N. A. Khan A. Samb Th1/Th2 Dichotomy in Obese Women with Gestational Diabetes and Their Macrosomic Babies Journal of Diabetes Research |
| title | Th1/Th2 Dichotomy in Obese Women with Gestational Diabetes and Their Macrosomic Babies |
| title_full | Th1/Th2 Dichotomy in Obese Women with Gestational Diabetes and Their Macrosomic Babies |
| title_fullStr | Th1/Th2 Dichotomy in Obese Women with Gestational Diabetes and Their Macrosomic Babies |
| title_full_unstemmed | Th1/Th2 Dichotomy in Obese Women with Gestational Diabetes and Their Macrosomic Babies |
| title_short | Th1/Th2 Dichotomy in Obese Women with Gestational Diabetes and Their Macrosomic Babies |
| title_sort | th1 th2 dichotomy in obese women with gestational diabetes and their macrosomic babies |
| url | http://dx.doi.org/10.1155/2018/8474617 |
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