Microencapsulated inulin as a prebiotic: Enhancing growth, digestive enzyme activity, and immune response in striped catfish (Pangasianodon hypophthalmus)

This study assessed the influence of dietary microencapsulated inulin on the physiological performance, enzymatic activity, immune modulation, antioxidant status, and tissue morphology of striped catfish (Pangasianodon hypophthalmus). Juvenile fish (initial weight: 5.06 ± 0.12 g; length: 6.93 ± 0.14...

Full description

Saved in:
Bibliographic Details
Main Authors: Supalug Kattakdad, Nittaya Phungam, Urailuck Pongket, Woranit Muangmala, Suriya Udduang, Siti Ariza Aripin, Bundit Yuangsoi
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Aquaculture Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2352513425003722
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849392476223176704
author Supalug Kattakdad
Nittaya Phungam
Urailuck Pongket
Woranit Muangmala
Suriya Udduang
Siti Ariza Aripin
Bundit Yuangsoi
author_facet Supalug Kattakdad
Nittaya Phungam
Urailuck Pongket
Woranit Muangmala
Suriya Udduang
Siti Ariza Aripin
Bundit Yuangsoi
author_sort Supalug Kattakdad
collection DOAJ
description This study assessed the influence of dietary microencapsulated inulin on the physiological performance, enzymatic activity, immune modulation, antioxidant status, and tissue morphology of striped catfish (Pangasianodon hypophthalmus). Juvenile fish (initial weight: 5.06 ± 0.12 g; length: 6.93 ± 0.14 cm) were reared under controlled conditions and fed diets containing 0 %, 0.4 %, 0.8 %, and 1.2 % inulin for eight weeks. Feeding was conducted thrice daily to apparent satiation. The 0.8 % inulin treatment yielded the most pronounced improvements in final body weight, and feed conversion ratio (p < 0.05), while survival and viscerosomatic index were unaffected. Hepatosomatic index increased significantly in fish receiving 0.8 % and 1.2 % inulin-based diets. Enhanced cellulase activity was observed with inulin inclusion (p < 0.05), whereas protease and amylase activities remained unchanged. Blood parameters, including erythrocyte, leukocyte, and hemoglobin levels, showed no notable variation; however, total plasma protein, immunoglobulin, lysozyme, and antioxidant enzymes (Superoxide dismutase and Glutathione peroxidase) increased significantly. Morphological enhancements in intestinal tissues such as villi architecture and goblet cell density were observed, along with improved liver histoarchitecture in inulin-fed groups. These results support the use of microencapsulated inulin, particularly at 0.8 %, as an effective functional feed additive to enhance growth, immune defense, and tissue integrity in striped catfish culture.
format Article
id doaj-art-8b60fabcb8c24bc299bfcca12b000e4a
institution Kabale University
issn 2352-5134
language English
publishDate 2025-09-01
publisher Elsevier
record_format Article
series Aquaculture Reports
spelling doaj-art-8b60fabcb8c24bc299bfcca12b000e4a2025-08-20T03:40:46ZengElsevierAquaculture Reports2352-51342025-09-014310298610.1016/j.aqrep.2025.102986Microencapsulated inulin as a prebiotic: Enhancing growth, digestive enzyme activity, and immune response in striped catfish (Pangasianodon hypophthalmus)Supalug Kattakdad0Nittaya Phungam1Urailuck Pongket2Woranit Muangmala3Suriya Udduang4Siti Ariza Aripin5Bundit Yuangsoi6Faculty of Agriculture and Technology, Rajamangala University of Technology Isan, Surin 32000, Thailand; Corresponding author.Faculty of Agriculture and Technology, Rajamangala University of Technology Isan, Surin 32000, ThailandFaculty of Agriculture and Technology, Rajamangala University of Technology Isan, Surin 32000, ThailandFaculty of Agriculture and Technology, Rajamangala University of Technology Isan, Surin 32000, ThailandFaculty of Agriculture and Technology, Rajamangala University of Technology Isan, Surin 32000, ThailandFaculty of Fisheries and Food Science, Universiti Malaysia Terengganu, Terengganu 21030, MalaysiaFaculty of Agriculture, Khon Kaen University, Khon Kaen 40002, ThailandThis study assessed the influence of dietary microencapsulated inulin on the physiological performance, enzymatic activity, immune modulation, antioxidant status, and tissue morphology of striped catfish (Pangasianodon hypophthalmus). Juvenile fish (initial weight: 5.06 ± 0.12 g; length: 6.93 ± 0.14 cm) were reared under controlled conditions and fed diets containing 0 %, 0.4 %, 0.8 %, and 1.2 % inulin for eight weeks. Feeding was conducted thrice daily to apparent satiation. The 0.8 % inulin treatment yielded the most pronounced improvements in final body weight, and feed conversion ratio (p < 0.05), while survival and viscerosomatic index were unaffected. Hepatosomatic index increased significantly in fish receiving 0.8 % and 1.2 % inulin-based diets. Enhanced cellulase activity was observed with inulin inclusion (p < 0.05), whereas protease and amylase activities remained unchanged. Blood parameters, including erythrocyte, leukocyte, and hemoglobin levels, showed no notable variation; however, total plasma protein, immunoglobulin, lysozyme, and antioxidant enzymes (Superoxide dismutase and Glutathione peroxidase) increased significantly. Morphological enhancements in intestinal tissues such as villi architecture and goblet cell density were observed, along with improved liver histoarchitecture in inulin-fed groups. These results support the use of microencapsulated inulin, particularly at 0.8 %, as an effective functional feed additive to enhance growth, immune defense, and tissue integrity in striped catfish culture.http://www.sciencedirect.com/science/article/pii/S2352513425003722Feed additiveImmune stimulantInulinPrebioticStriped catfish
spellingShingle Supalug Kattakdad
Nittaya Phungam
Urailuck Pongket
Woranit Muangmala
Suriya Udduang
Siti Ariza Aripin
Bundit Yuangsoi
Microencapsulated inulin as a prebiotic: Enhancing growth, digestive enzyme activity, and immune response in striped catfish (Pangasianodon hypophthalmus)
Aquaculture Reports
Feed additive
Immune stimulant
Inulin
Prebiotic
Striped catfish
title Microencapsulated inulin as a prebiotic: Enhancing growth, digestive enzyme activity, and immune response in striped catfish (Pangasianodon hypophthalmus)
title_full Microencapsulated inulin as a prebiotic: Enhancing growth, digestive enzyme activity, and immune response in striped catfish (Pangasianodon hypophthalmus)
title_fullStr Microencapsulated inulin as a prebiotic: Enhancing growth, digestive enzyme activity, and immune response in striped catfish (Pangasianodon hypophthalmus)
title_full_unstemmed Microencapsulated inulin as a prebiotic: Enhancing growth, digestive enzyme activity, and immune response in striped catfish (Pangasianodon hypophthalmus)
title_short Microencapsulated inulin as a prebiotic: Enhancing growth, digestive enzyme activity, and immune response in striped catfish (Pangasianodon hypophthalmus)
title_sort microencapsulated inulin as a prebiotic enhancing growth digestive enzyme activity and immune response in striped catfish pangasianodon hypophthalmus
topic Feed additive
Immune stimulant
Inulin
Prebiotic
Striped catfish
url http://www.sciencedirect.com/science/article/pii/S2352513425003722
work_keys_str_mv AT supalugkattakdad microencapsulatedinulinasaprebioticenhancinggrowthdigestiveenzymeactivityandimmuneresponseinstripedcatfishpangasianodonhypophthalmus
AT nittayaphungam microencapsulatedinulinasaprebioticenhancinggrowthdigestiveenzymeactivityandimmuneresponseinstripedcatfishpangasianodonhypophthalmus
AT urailuckpongket microencapsulatedinulinasaprebioticenhancinggrowthdigestiveenzymeactivityandimmuneresponseinstripedcatfishpangasianodonhypophthalmus
AT woranitmuangmala microencapsulatedinulinasaprebioticenhancinggrowthdigestiveenzymeactivityandimmuneresponseinstripedcatfishpangasianodonhypophthalmus
AT suriyaudduang microencapsulatedinulinasaprebioticenhancinggrowthdigestiveenzymeactivityandimmuneresponseinstripedcatfishpangasianodonhypophthalmus
AT sitiarizaaripin microencapsulatedinulinasaprebioticenhancinggrowthdigestiveenzymeactivityandimmuneresponseinstripedcatfishpangasianodonhypophthalmus
AT bundityuangsoi microencapsulatedinulinasaprebioticenhancinggrowthdigestiveenzymeactivityandimmuneresponseinstripedcatfishpangasianodonhypophthalmus