PCBP2 as an intrinsic agi ng factor regulates the senescence of hBMSCs through the ROS-FGF2 signaling axis
Background: It has been reported that loss of PCBP2 led to increased reactive oxygen species (ROS) production and accelerated cell aging. Knockdown of PCBP2 in HCT116 cells leads to significant downregulation of fibroblast growth factor 2 (FGF2). Here, we tried to elucidate the intrinsic factors and...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2025-03-01
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| Online Access: | https://elifesciences.org/articles/92419 |
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| author | Pengbo Chen Bo Li Zeyu Lu Qingyin Xu Huoliang Zheng Shengdan Jiang Leisheng Jiang Xinfeng Zheng |
| author_facet | Pengbo Chen Bo Li Zeyu Lu Qingyin Xu Huoliang Zheng Shengdan Jiang Leisheng Jiang Xinfeng Zheng |
| author_sort | Pengbo Chen |
| collection | DOAJ |
| description | Background: It has been reported that loss of PCBP2 led to increased reactive oxygen species (ROS) production and accelerated cell aging. Knockdown of PCBP2 in HCT116 cells leads to significant downregulation of fibroblast growth factor 2 (FGF2). Here, we tried to elucidate the intrinsic factors and potential mechanisms of bone marrow mesenchymal stromal cells (BMSCs) aging from the interactions among PCBP2, ROS, and FGF2.
Methods: Unlabeled quantitative proteomics were performed to show differentially expressed proteins in the replicative senescent human bone marrow mesenchymal stromal cells (RS-hBMSCs). ROS and FGF2 were detected in the loss-and-gain cell function experiments of PCBP2. The functional recovery experiments were performed to verify whether PCBP2 regulates cell function through ROS/FGF2-dependent ways.
Results: PCBP2 expression was significantly lower in P10-hBMSCs. Knocking down the expression of PCBP2 inhibited the proliferation while accentuated the apoptosis and cell arrest of RS-hBMSCs. PCBP2 silence could increase the production of ROS. On the contrary, overexpression of PCBP2 increased the viability of both P3-hBMSCs and P10-hBMSCs significantly. Meanwhile, overexpression of PCBP2 led to significantly reduced expression of FGF2. Overexpression of FGF2 significantly offset the effect of PCBP2 overexpression in P10-hBMSCs, leading to decreased cell proliferation, increased apoptosis, and reduced G0/G1 phase ratio of the cells.
Conclusions: This study initially elucidates that PCBP2 as an intrinsic aging factor regulates the replicative senescence of hBMSCs through the ROS-FGF2 signaling axis.
Funding: This study was supported by the National Natural Science Foundation of China (82172474). |
| format | Article |
| id | doaj-art-8b4d15a4dbb4416aac9f5a0640d1acf7 |
| institution | DOAJ |
| issn | 2050-084X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | eLife Sciences Publications Ltd |
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| series | eLife |
| spelling | doaj-art-8b4d15a4dbb4416aac9f5a0640d1acf72025-08-20T03:06:04ZengeLife Sciences Publications LtdeLife2050-084X2025-03-011310.7554/eLife.92419PCBP2 as an intrinsic agi ng factor regulates the senescence of hBMSCs through the ROS-FGF2 signaling axisPengbo Chen0Bo Li1Zeyu Lu2https://orcid.org/0000-0002-3051-9183Qingyin Xu3Huoliang Zheng4Shengdan Jiang5Leisheng Jiang6Xinfeng Zheng7https://orcid.org/0000-0003-0837-785XSpine Center, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaSpine Center, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaSpine Center, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaSpine Center, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaSpine Center, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaSpine Center, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaSpine Center, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaSpine Center, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaBackground: It has been reported that loss of PCBP2 led to increased reactive oxygen species (ROS) production and accelerated cell aging. Knockdown of PCBP2 in HCT116 cells leads to significant downregulation of fibroblast growth factor 2 (FGF2). Here, we tried to elucidate the intrinsic factors and potential mechanisms of bone marrow mesenchymal stromal cells (BMSCs) aging from the interactions among PCBP2, ROS, and FGF2. Methods: Unlabeled quantitative proteomics were performed to show differentially expressed proteins in the replicative senescent human bone marrow mesenchymal stromal cells (RS-hBMSCs). ROS and FGF2 were detected in the loss-and-gain cell function experiments of PCBP2. The functional recovery experiments were performed to verify whether PCBP2 regulates cell function through ROS/FGF2-dependent ways. Results: PCBP2 expression was significantly lower in P10-hBMSCs. Knocking down the expression of PCBP2 inhibited the proliferation while accentuated the apoptosis and cell arrest of RS-hBMSCs. PCBP2 silence could increase the production of ROS. On the contrary, overexpression of PCBP2 increased the viability of both P3-hBMSCs and P10-hBMSCs significantly. Meanwhile, overexpression of PCBP2 led to significantly reduced expression of FGF2. Overexpression of FGF2 significantly offset the effect of PCBP2 overexpression in P10-hBMSCs, leading to decreased cell proliferation, increased apoptosis, and reduced G0/G1 phase ratio of the cells. Conclusions: This study initially elucidates that PCBP2 as an intrinsic aging factor regulates the replicative senescence of hBMSCs through the ROS-FGF2 signaling axis. Funding: This study was supported by the National Natural Science Foundation of China (82172474).https://elifesciences.org/articles/92419osteoporosissenescencehBMSCsPCBP2ROSFGF2 |
| spellingShingle | Pengbo Chen Bo Li Zeyu Lu Qingyin Xu Huoliang Zheng Shengdan Jiang Leisheng Jiang Xinfeng Zheng PCBP2 as an intrinsic agi ng factor regulates the senescence of hBMSCs through the ROS-FGF2 signaling axis eLife osteoporosis senescence hBMSCs PCBP2 ROS FGF2 |
| title | PCBP2 as an intrinsic agi ng factor regulates the senescence of hBMSCs through the ROS-FGF2 signaling axis |
| title_full | PCBP2 as an intrinsic agi ng factor regulates the senescence of hBMSCs through the ROS-FGF2 signaling axis |
| title_fullStr | PCBP2 as an intrinsic agi ng factor regulates the senescence of hBMSCs through the ROS-FGF2 signaling axis |
| title_full_unstemmed | PCBP2 as an intrinsic agi ng factor regulates the senescence of hBMSCs through the ROS-FGF2 signaling axis |
| title_short | PCBP2 as an intrinsic agi ng factor regulates the senescence of hBMSCs through the ROS-FGF2 signaling axis |
| title_sort | pcbp2 as an intrinsic agi ng factor regulates the senescence of hbmscs through the ros fgf2 signaling axis |
| topic | osteoporosis senescence hBMSCs PCBP2 ROS FGF2 |
| url | https://elifesciences.org/articles/92419 |
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