Progressive gut microbiota shifts and functional alterations across aging stages and frailty in mice
Summary: The gut microbiota modulates aging through metabolic and immune interactions. To characterize microbial changes during aging, we profiled gut microbiota across four stages in male C57BL/6J mice: young-adult, middle-aged, senescent, and frailty. Using 16S rRNA sequencing and targeted metabol...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-07-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225012465 |
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| Summary: | Summary: The gut microbiota modulates aging through metabolic and immune interactions. To characterize microbial changes during aging, we profiled gut microbiota across four stages in male C57BL/6J mice: young-adult, middle-aged, senescent, and frailty. Using 16S rRNA sequencing and targeted metabolomics, we observed a biphasic diversity pattern, with reduced richness in senescence followed by a rebound in frailty. Frailty-stage microbiota showed increased connectivity and enrichment of Lachnospiraceae, alongside short-chain fatty acid (SCFA) depletion, particularly butanoic acid. Functional predictions revealed elevated immune activity and reduced carbohydrate metabolism, linked to specific microbial taxa. These findings highlight a structured remodeling of the gut microbiota during aging, with frailty representing a distinct dysbiotic state associated with metabolic dysfunction. Modulating gut microbial composition may represent a potential strategy to attenuate frailty and promote healthy aging. |
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| ISSN: | 2589-0042 |