Brucella abortus impairs T lymphocyte responsiveness by mobilizing IL-1RA-secreting omental neutrophils

Abstract Immune evasion strategies of Brucella, the etiologic agent of brucellosis, a global zoonosis, remain partially understood. The omentum, a tertiary lymphoid organ part of visceral adipose tissue, has never been explored as a Brucella reservoir. We report that B. abortus infects and replicate...

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Main Authors: Joaquin M. Pellegrini, Gabriela González-Espinoza, Raheleh R. Shayan, Lisiena Hysenaj, Thomas Rouma, Vilma Arce-Gorvel, Hugues Lelouard, Dimitri Popoff, Yun Zhao, Sean Hanniffy, Amanda Castillo-Zeledón, Maite Loperena-Barber, Javier Celis-Gutierrez, Cyrille Mionnet, Mile Bosilkovski, Javier Solera, Eric Muraille, Elías Barquero-Calvo, Edgardo Moreno, Raquel Conde-Álvarez, Ignacio Moriyón, Jean-Pierre Gorvel, Sylvie Mémet
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-024-55799-2
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author Joaquin M. Pellegrini
Gabriela González-Espinoza
Raheleh R. Shayan
Lisiena Hysenaj
Thomas Rouma
Vilma Arce-Gorvel
Hugues Lelouard
Dimitri Popoff
Yun Zhao
Sean Hanniffy
Amanda Castillo-Zeledón
Maite Loperena-Barber
Javier Celis-Gutierrez
Cyrille Mionnet
Mile Bosilkovski
Javier Solera
Eric Muraille
Elías Barquero-Calvo
Edgardo Moreno
Raquel Conde-Álvarez
Ignacio Moriyón
Jean-Pierre Gorvel
Sylvie Mémet
author_facet Joaquin M. Pellegrini
Gabriela González-Espinoza
Raheleh R. Shayan
Lisiena Hysenaj
Thomas Rouma
Vilma Arce-Gorvel
Hugues Lelouard
Dimitri Popoff
Yun Zhao
Sean Hanniffy
Amanda Castillo-Zeledón
Maite Loperena-Barber
Javier Celis-Gutierrez
Cyrille Mionnet
Mile Bosilkovski
Javier Solera
Eric Muraille
Elías Barquero-Calvo
Edgardo Moreno
Raquel Conde-Álvarez
Ignacio Moriyón
Jean-Pierre Gorvel
Sylvie Mémet
author_sort Joaquin M. Pellegrini
collection DOAJ
description Abstract Immune evasion strategies of Brucella, the etiologic agent of brucellosis, a global zoonosis, remain partially understood. The omentum, a tertiary lymphoid organ part of visceral adipose tissue, has never been explored as a Brucella reservoir. We report that B. abortus infects and replicates within murine omental macrophages. Throughout the chronic phase of infection, the omentum accumulates macrophages, monocytes and neutrophils. The maintenance of PD-L1+Sca-1+ macrophages, monocytes and neutrophils in the omentum depends on the wadC-encoded determinant of Brucella LPS. We demonstrate that PD-L1+Sca-1+ murine omental neutrophils produce high levels of IL-1RA leading to T cell hyporesponsiveness. These findings corroborate brucellosis patient analysis of whole blood displaying upregulation of PDL1 and Ly6E genes, and of serum exhibiting high levels of IL-1RA. Overall, the omentum, a reservoir for B. abortus, promotes bacterial persistence and causes CD4+ and CD8+ T cell immunosuppression by IL-1RA secreted by PD-L1+Sca-1+ neutrophils.
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spelling doaj-art-8b32eea2b7ff42bcaf3cbd9d17a636662025-08-20T02:48:12ZengNature PortfolioNature Communications2041-17232025-01-0116111610.1038/s41467-024-55799-2Brucella abortus impairs T lymphocyte responsiveness by mobilizing IL-1RA-secreting omental neutrophilsJoaquin M. Pellegrini0Gabriela González-Espinoza1Raheleh R. Shayan2Lisiena Hysenaj3Thomas Rouma4Vilma Arce-Gorvel5Hugues Lelouard6Dimitri Popoff7Yun Zhao8Sean Hanniffy9Amanda Castillo-Zeledón10Maite Loperena-Barber11Javier Celis-Gutierrez12Cyrille Mionnet13Mile Bosilkovski14Javier Solera15Eric Muraille16Elías Barquero-Calvo17Edgardo Moreno18Raquel Conde-Álvarez19Ignacio Moriyón20Jean-Pierre Gorvel21Sylvie Mémet22Aix Marseille Université, CNRS, INSERM, CIMLAix Marseille Université, CNRS, INSERM, CIMLAix Marseille Université, CNRS, INSERM, CIMLAix Marseille Université, CNRS, INSERM, CIMLUnité de Recherche en Biologie des Microorganismes (URBM)-Laboratoire d’Immunologie et de Microbiologie, NARILIS, University of NamurAix Marseille Université, CNRS, INSERM, CIMLAix Marseille Université, CNRS, INSERM, CIMLAix Marseille Université, CNRS, INSERM, CIMLAix Marseille Université, CNRS, INSERM, CIMLAix Marseille Université, CNRS, INSERM, CIMLUniversidad Nacional, Pathology Department, Escuela de Medicina VeterinariaUniversidad de Navarra, Instituto de Salud Tropical e Departamento de Microbiología y ParasitologíaAix Marseille Université, CNRS, INSERM, CIMLAix Marseille Université, CNRS, INSERM, CIMLUniversity Clinic for Infectious Diseases and Febrile ConditionsHospital General Universitario, Facultad de Medicina, Universidad Castilla la Mancha AlbaceteUnité de Recherche en Biologie des Microorganismes (URBM)-Laboratoire d’Immunologie et de Microbiologie, NARILIS, University of NamurUniversidad Nacional, Pathology Department, Escuela de Medicina VeterinariaUniversidad Nacional, Pathology Department, Escuela de Medicina VeterinariaUniversidad de Navarra, Instituto de Salud Tropical e Departamento de Microbiología y ParasitologíaUniversidad de Navarra, Instituto de Salud Tropical e Departamento de Microbiología y ParasitologíaAix Marseille Université, CNRS, INSERM, CIMLAix Marseille Université, CNRS, INSERM, CIMLAbstract Immune evasion strategies of Brucella, the etiologic agent of brucellosis, a global zoonosis, remain partially understood. The omentum, a tertiary lymphoid organ part of visceral adipose tissue, has never been explored as a Brucella reservoir. We report that B. abortus infects and replicates within murine omental macrophages. Throughout the chronic phase of infection, the omentum accumulates macrophages, monocytes and neutrophils. The maintenance of PD-L1+Sca-1+ macrophages, monocytes and neutrophils in the omentum depends on the wadC-encoded determinant of Brucella LPS. We demonstrate that PD-L1+Sca-1+ murine omental neutrophils produce high levels of IL-1RA leading to T cell hyporesponsiveness. These findings corroborate brucellosis patient analysis of whole blood displaying upregulation of PDL1 and Ly6E genes, and of serum exhibiting high levels of IL-1RA. Overall, the omentum, a reservoir for B. abortus, promotes bacterial persistence and causes CD4+ and CD8+ T cell immunosuppression by IL-1RA secreted by PD-L1+Sca-1+ neutrophils.https://doi.org/10.1038/s41467-024-55799-2
spellingShingle Joaquin M. Pellegrini
Gabriela González-Espinoza
Raheleh R. Shayan
Lisiena Hysenaj
Thomas Rouma
Vilma Arce-Gorvel
Hugues Lelouard
Dimitri Popoff
Yun Zhao
Sean Hanniffy
Amanda Castillo-Zeledón
Maite Loperena-Barber
Javier Celis-Gutierrez
Cyrille Mionnet
Mile Bosilkovski
Javier Solera
Eric Muraille
Elías Barquero-Calvo
Edgardo Moreno
Raquel Conde-Álvarez
Ignacio Moriyón
Jean-Pierre Gorvel
Sylvie Mémet
Brucella abortus impairs T lymphocyte responsiveness by mobilizing IL-1RA-secreting omental neutrophils
Nature Communications
title Brucella abortus impairs T lymphocyte responsiveness by mobilizing IL-1RA-secreting omental neutrophils
title_full Brucella abortus impairs T lymphocyte responsiveness by mobilizing IL-1RA-secreting omental neutrophils
title_fullStr Brucella abortus impairs T lymphocyte responsiveness by mobilizing IL-1RA-secreting omental neutrophils
title_full_unstemmed Brucella abortus impairs T lymphocyte responsiveness by mobilizing IL-1RA-secreting omental neutrophils
title_short Brucella abortus impairs T lymphocyte responsiveness by mobilizing IL-1RA-secreting omental neutrophils
title_sort brucella abortus impairs t lymphocyte responsiveness by mobilizing il 1ra secreting omental neutrophils
url https://doi.org/10.1038/s41467-024-55799-2
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