Myeloid-derived suppressor cells exhibit distinct characteristics in bone marrow and blood of individuals with diffuse large B-cell lymphoma
Antitumor immune surveillance is the key feature of tumour progression and response to treatment in various malignancies, such as lymphomas. Myeloid derived suppressor cells (MDSCs) are bone marrow (BM)-derived cells with potent suppressive properties, implicated in T cell inhibition and tumour diss...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2024.1515097/full |
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| author | Paris Efstratiou Athina Damianaki Aglaia Kavidopoulou Polymnia Ioannidou Effrosyni Markaki Ioannis Moysis Skianis Electra Tsagliotis Vasilia Kaliafentaki Angelos Mattheakakis Maria Ximeri Eleftherios Manouras Matthieu Lavigne Panayotis Verginis Panayotis Verginis Panayotis Verginis Christina Kalpadakis |
| author_facet | Paris Efstratiou Athina Damianaki Aglaia Kavidopoulou Polymnia Ioannidou Effrosyni Markaki Ioannis Moysis Skianis Electra Tsagliotis Vasilia Kaliafentaki Angelos Mattheakakis Maria Ximeri Eleftherios Manouras Matthieu Lavigne Panayotis Verginis Panayotis Verginis Panayotis Verginis Christina Kalpadakis |
| author_sort | Paris Efstratiou |
| collection | DOAJ |
| description | Antitumor immune surveillance is the key feature of tumour progression and response to treatment in various malignancies, such as lymphomas. Myeloid derived suppressor cells (MDSCs) are bone marrow (BM)-derived cells with potent suppressive properties, implicated in T cell inhibition and tumour dissemination. In Diffuse Large B-cell Lymphoma (DLBCL), circulating MDSCs constitute the immunosuppressive tumor microenvironment, while the contribution of BM MDSCs in disease pathogenesis remains elusive. In the present study we aimed to evaluate both the frequencies as well as the molecular signatures of MDSCs in blood and BM from newly diagnosed DLBCL patients prior to treatment initiation and from age matched healthy donors. Circulating levels of total, monocytic (M-) and polymorphonuclear (PMN-) MDSCs were found increased in DLBCL compared to healthy control, while in DLBCL patients the BM MDSCs were significantly increased compared to blood. Transcriptomic analysis revealed significantly different molecular fingerprints to characterize circulating and BM M-MDSCs, implying that MDSCs exhibit their function with distinct mechanisms depending on the anatomical compartment. Despite that MDSC frequencies did not demonstrate any significant correlation with disease characteristics and outcome, our findings propose that gene expression profiling should be evaluated for their potential prognostic impact. Overall, the findings presented here, provide new insights in the immunosuppressive networks that operate in DLBCL and importantly propose new molecular mechanisms expressed by BM MDSCs which may be explored therapeutically. |
| format | Article |
| id | doaj-art-8b1a1fff81194035b7ce699ba798a78a |
| institution | Kabale University |
| issn | 2296-858X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj-art-8b1a1fff81194035b7ce699ba798a78a2025-01-29T06:46:15ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2025-01-011110.3389/fmed.2024.15150971515097Myeloid-derived suppressor cells exhibit distinct characteristics in bone marrow and blood of individuals with diffuse large B-cell lymphomaParis Efstratiou0Athina Damianaki1Aglaia Kavidopoulou2Polymnia Ioannidou3Effrosyni Markaki4Ioannis Moysis Skianis5Electra Tsagliotis6Vasilia Kaliafentaki7Angelos Mattheakakis8Maria Ximeri9Eleftherios Manouras10Matthieu Lavigne11Panayotis Verginis12Panayotis Verginis13Panayotis Verginis14Christina Kalpadakis15Laboratory of Hematology, Division of Laboratory Medicine, School of Medicine, University of Crete and University Hospital of Heraklion, Heraklion, GreeceLaboratory of Hematology, Division of Laboratory Medicine, School of Medicine, University of Crete and University Hospital of Heraklion, Heraklion, GreeceLaboratory of Hematology, Division of Laboratory Medicine, School of Medicine, University of Crete and University Hospital of Heraklion, Heraklion, GreeceLaboratory of Hematology, Division of Laboratory Medicine, School of Medicine, University of Crete and University Hospital of Heraklion, Heraklion, GreeceLaboratory of Immune Regulation and Tolerance, Division of Basic Sciences, School of Medicine, University of Crete, Heraklion, GreeceLaboratory of Hematology, Division of Laboratory Medicine, School of Medicine, University of Crete and University Hospital of Heraklion, Heraklion, GreeceInstitute of Molecular Biology and Biotechnology (IMBB), Foundation for Research and Technology-Hellas (FORTH), Heraklion, GreeceLaboratory of Hematology, Division of Laboratory Medicine, School of Medicine, University of Crete and University Hospital of Heraklion, Heraklion, GreeceLaboratory of Hematology, Division of Laboratory Medicine, School of Medicine, University of Crete and University Hospital of Heraklion, Heraklion, GreeceLaboratory of Hematology, Division of Laboratory Medicine, School of Medicine, University of Crete and University Hospital of Heraklion, Heraklion, GreeceLaboratory of Hematology, Division of Laboratory Medicine, School of Medicine, University of Crete and University Hospital of Heraklion, Heraklion, GreeceInstitute of Molecular Biology and Biotechnology (IMBB), Foundation for Research and Technology-Hellas (FORTH), Heraklion, GreeceLaboratory of Hematology, Division of Laboratory Medicine, School of Medicine, University of Crete and University Hospital of Heraklion, Heraklion, GreeceLaboratory of Immune Regulation and Tolerance, Division of Basic Sciences, School of Medicine, University of Crete, Heraklion, GreeceInstitute of Molecular Biology and Biotechnology (IMBB), Foundation for Research and Technology-Hellas (FORTH), Heraklion, GreeceLaboratory of Hematology, Division of Laboratory Medicine, School of Medicine, University of Crete and University Hospital of Heraklion, Heraklion, GreeceAntitumor immune surveillance is the key feature of tumour progression and response to treatment in various malignancies, such as lymphomas. Myeloid derived suppressor cells (MDSCs) are bone marrow (BM)-derived cells with potent suppressive properties, implicated in T cell inhibition and tumour dissemination. In Diffuse Large B-cell Lymphoma (DLBCL), circulating MDSCs constitute the immunosuppressive tumor microenvironment, while the contribution of BM MDSCs in disease pathogenesis remains elusive. In the present study we aimed to evaluate both the frequencies as well as the molecular signatures of MDSCs in blood and BM from newly diagnosed DLBCL patients prior to treatment initiation and from age matched healthy donors. Circulating levels of total, monocytic (M-) and polymorphonuclear (PMN-) MDSCs were found increased in DLBCL compared to healthy control, while in DLBCL patients the BM MDSCs were significantly increased compared to blood. Transcriptomic analysis revealed significantly different molecular fingerprints to characterize circulating and BM M-MDSCs, implying that MDSCs exhibit their function with distinct mechanisms depending on the anatomical compartment. Despite that MDSC frequencies did not demonstrate any significant correlation with disease characteristics and outcome, our findings propose that gene expression profiling should be evaluated for their potential prognostic impact. Overall, the findings presented here, provide new insights in the immunosuppressive networks that operate in DLBCL and importantly propose new molecular mechanisms expressed by BM MDSCs which may be explored therapeutically.https://www.frontiersin.org/articles/10.3389/fmed.2024.1515097/fullDLBCLMDSCsbone marrowlymphomastranscriptomic analysis |
| spellingShingle | Paris Efstratiou Athina Damianaki Aglaia Kavidopoulou Polymnia Ioannidou Effrosyni Markaki Ioannis Moysis Skianis Electra Tsagliotis Vasilia Kaliafentaki Angelos Mattheakakis Maria Ximeri Eleftherios Manouras Matthieu Lavigne Panayotis Verginis Panayotis Verginis Panayotis Verginis Christina Kalpadakis Myeloid-derived suppressor cells exhibit distinct characteristics in bone marrow and blood of individuals with diffuse large B-cell lymphoma Frontiers in Medicine DLBCL MDSCs bone marrow lymphomas transcriptomic analysis |
| title | Myeloid-derived suppressor cells exhibit distinct characteristics in bone marrow and blood of individuals with diffuse large B-cell lymphoma |
| title_full | Myeloid-derived suppressor cells exhibit distinct characteristics in bone marrow and blood of individuals with diffuse large B-cell lymphoma |
| title_fullStr | Myeloid-derived suppressor cells exhibit distinct characteristics in bone marrow and blood of individuals with diffuse large B-cell lymphoma |
| title_full_unstemmed | Myeloid-derived suppressor cells exhibit distinct characteristics in bone marrow and blood of individuals with diffuse large B-cell lymphoma |
| title_short | Myeloid-derived suppressor cells exhibit distinct characteristics in bone marrow and blood of individuals with diffuse large B-cell lymphoma |
| title_sort | myeloid derived suppressor cells exhibit distinct characteristics in bone marrow and blood of individuals with diffuse large b cell lymphoma |
| topic | DLBCL MDSCs bone marrow lymphomas transcriptomic analysis |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2024.1515097/full |
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