Genetic and biochemical insights into siderophore biosynthesis in the marine fish pathogen Tenacibaculum maritimum
Abstract Siderophore production is a key fitness trait for bacteria, particularly in iron-limited environments such as marine ecosystems and host tissues. In this study, we identify the tenABECDC2D2hp1-4 gene cluster as responsible for siderophore biosynthesis in Tenacibaculum maritimum, confirmed t...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-08217-6 |
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| Summary: | Abstract Siderophore production is a key fitness trait for bacteria, particularly in iron-limited environments such as marine ecosystems and host tissues. In this study, we identify the tenABECDC2D2hp1-4 gene cluster as responsible for siderophore biosynthesis in Tenacibaculum maritimum, confirmed through genome analysis and a tenCD-inactivated mutant. This cluster, highly similar to the desferrioxamine biosynthesis system in Streptomyces coelicolor, features a unique tenCD duplication/fusion, essential for siderophore formation. Additionally, accessory genes (hp1-4) encode functions such as nitroreductase and N-acetyltransferase, likely contributing to siderophore diversification. LC/MS analysis of T. maritimum cultures revealed the production of 20 amphiphilic, acylated desferrioxamine-like siderophores. These findings provide new insights into the genetic and metabolic versatility of marine pathogens in iron acquisition. |
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| ISSN: | 2045-2322 |