Th17 Cytokines Disrupt the Airway Mucosal Barrier in Chronic Rhinosinusitis
Cytokine mediated changes in paracellular permeability contribute to a multitude of pathological conditions including chronic rhinosinusitis (CRS). The purpose of this study was to investigate the effect of interferons and of Th1, Th2, and Th17 cytokines on respiratory epithelium barrier function. C...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/9798206 |
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| author | Mahnaz Ramezanpour Sophia Moraitis Jason L. P. Smith P. J. Wormald Sarah Vreugde |
| author_facet | Mahnaz Ramezanpour Sophia Moraitis Jason L. P. Smith P. J. Wormald Sarah Vreugde |
| author_sort | Mahnaz Ramezanpour |
| collection | DOAJ |
| description | Cytokine mediated changes in paracellular permeability contribute to a multitude of pathological conditions including chronic rhinosinusitis (CRS). The purpose of this study was to investigate the effect of interferons and of Th1, Th2, and Th17 cytokines on respiratory epithelium barrier function. Cytokines and interferons were applied to the basolateral side of air-liquid interface (ALI) cultures of primary human nasal epithelial cells (HNECs) from CRS with nasal polyp patients. Transepithelial electrical resistance (TEER) and permeability of FITC-conjugated dextrans were measured over time. Additionally, the expression of the tight junction protein Zona Occludens-1 (ZO-1) was examined via immunofluorescence. Data was analysed using ANOVA, followed by Tukey HSD post hoc test. Our results showed that application of interferons and of Th1 or Th2 cytokines did not affect the mucosal barrier function. In contrast, the Th17 cytokines IL-17, IL-22, and IL-26 showed a significant disruption of the epithelial barrier, evidenced by a loss of TEER, increased paracellular permeability of FITC-dextrans, and discontinuous ZO-1 immunolocalisation. These results indicate that Th17 cytokines may contribute to the development of CRSwNP by promoting a leaky mucosal barrier. |
| format | Article |
| id | doaj-art-8b1126e2129b412ba4c18ccc28be0573 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-8b1126e2129b412ba4c18ccc28be05732025-08-20T02:09:27ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/97982069798206Th17 Cytokines Disrupt the Airway Mucosal Barrier in Chronic RhinosinusitisMahnaz Ramezanpour0Sophia Moraitis1Jason L. P. Smith2P. J. Wormald3Sarah Vreugde4Department of Surgery (Otorhinolaryngology Head and Neck Surgery), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide, SA 5011, AustraliaDepartment of Surgery (Otorhinolaryngology Head and Neck Surgery), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide, SA 5011, AustraliaSchool of Biology, Faculty of Science and Engineering, Flinders University of South Australia, Adelaide, SA 5042, AustraliaDepartment of Surgery (Otorhinolaryngology Head and Neck Surgery), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide, SA 5011, AustraliaDepartment of Surgery (Otorhinolaryngology Head and Neck Surgery), The Queen Elizabeth Hospital and The University of Adelaide, Adelaide, SA 5011, AustraliaCytokine mediated changes in paracellular permeability contribute to a multitude of pathological conditions including chronic rhinosinusitis (CRS). The purpose of this study was to investigate the effect of interferons and of Th1, Th2, and Th17 cytokines on respiratory epithelium barrier function. Cytokines and interferons were applied to the basolateral side of air-liquid interface (ALI) cultures of primary human nasal epithelial cells (HNECs) from CRS with nasal polyp patients. Transepithelial electrical resistance (TEER) and permeability of FITC-conjugated dextrans were measured over time. Additionally, the expression of the tight junction protein Zona Occludens-1 (ZO-1) was examined via immunofluorescence. Data was analysed using ANOVA, followed by Tukey HSD post hoc test. Our results showed that application of interferons and of Th1 or Th2 cytokines did not affect the mucosal barrier function. In contrast, the Th17 cytokines IL-17, IL-22, and IL-26 showed a significant disruption of the epithelial barrier, evidenced by a loss of TEER, increased paracellular permeability of FITC-dextrans, and discontinuous ZO-1 immunolocalisation. These results indicate that Th17 cytokines may contribute to the development of CRSwNP by promoting a leaky mucosal barrier.http://dx.doi.org/10.1155/2016/9798206 |
| spellingShingle | Mahnaz Ramezanpour Sophia Moraitis Jason L. P. Smith P. J. Wormald Sarah Vreugde Th17 Cytokines Disrupt the Airway Mucosal Barrier in Chronic Rhinosinusitis Mediators of Inflammation |
| title | Th17 Cytokines Disrupt the Airway Mucosal Barrier in Chronic Rhinosinusitis |
| title_full | Th17 Cytokines Disrupt the Airway Mucosal Barrier in Chronic Rhinosinusitis |
| title_fullStr | Th17 Cytokines Disrupt the Airway Mucosal Barrier in Chronic Rhinosinusitis |
| title_full_unstemmed | Th17 Cytokines Disrupt the Airway Mucosal Barrier in Chronic Rhinosinusitis |
| title_short | Th17 Cytokines Disrupt the Airway Mucosal Barrier in Chronic Rhinosinusitis |
| title_sort | th17 cytokines disrupt the airway mucosal barrier in chronic rhinosinusitis |
| url | http://dx.doi.org/10.1155/2016/9798206 |
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