Tutorial on high-performance liquid chromatography of isotopologues of organic compounds
Separation of isotopologues or chemical species having different number of neutrons in one or more atoms of specific compound represents an important but difficult task. Application of high-performance liquid chromatography (HPLC) provides some flexibility in various separation modes of isotopologue...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-05-01
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| Series: | Journal of Chromatography Open |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772391725000052 |
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| author | P.N. Nesterenko V.D. Kazakova |
| author_facet | P.N. Nesterenko V.D. Kazakova |
| author_sort | P.N. Nesterenko |
| collection | DOAJ |
| description | Separation of isotopologues or chemical species having different number of neutrons in one or more atoms of specific compound represents an important but difficult task. Application of high-performance liquid chromatography (HPLC) provides some flexibility in various separation modes of isotopologues via different isotopic effects. This tutorial describes basic isotopic effects (hydrophobicity, polarizability, acid-base properties, size difference, etc.), which can influence HPLC performance in the separation of isotopologues. The article is focused on separation selectivity of deuterated organic compounds, for which different properties are considered. The use of advanced adsorption materials and techniques is discussed. |
| format | Article |
| id | doaj-art-8b0c29e6fb594458bc8f19c977eb11cb |
| institution | DOAJ |
| issn | 2772-3917 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Chromatography Open |
| spelling | doaj-art-8b0c29e6fb594458bc8f19c977eb11cb2025-08-20T03:00:50ZengElsevierJournal of Chromatography Open2772-39172025-05-01710020710.1016/j.jcoa.2025.100207Tutorial on high-performance liquid chromatography of isotopologues of organic compoundsP.N. Nesterenko0V.D. Kazakova1Corresponding author.; Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, GSP-1, Moscow, 119991, RussiaDepartment of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, GSP-1, Moscow, 119991, RussiaSeparation of isotopologues or chemical species having different number of neutrons in one or more atoms of specific compound represents an important but difficult task. Application of high-performance liquid chromatography (HPLC) provides some flexibility in various separation modes of isotopologues via different isotopic effects. This tutorial describes basic isotopic effects (hydrophobicity, polarizability, acid-base properties, size difference, etc.), which can influence HPLC performance in the separation of isotopologues. The article is focused on separation selectivity of deuterated organic compounds, for which different properties are considered. The use of advanced adsorption materials and techniques is discussed.http://www.sciencedirect.com/science/article/pii/S2772391725000052Liquid chromatographyIsotopologuesRetention mechanismSeparation selectivityIsotope effects |
| spellingShingle | P.N. Nesterenko V.D. Kazakova Tutorial on high-performance liquid chromatography of isotopologues of organic compounds Journal of Chromatography Open Liquid chromatography Isotopologues Retention mechanism Separation selectivity Isotope effects |
| title | Tutorial on high-performance liquid chromatography of isotopologues of organic compounds |
| title_full | Tutorial on high-performance liquid chromatography of isotopologues of organic compounds |
| title_fullStr | Tutorial on high-performance liquid chromatography of isotopologues of organic compounds |
| title_full_unstemmed | Tutorial on high-performance liquid chromatography of isotopologues of organic compounds |
| title_short | Tutorial on high-performance liquid chromatography of isotopologues of organic compounds |
| title_sort | tutorial on high performance liquid chromatography of isotopologues of organic compounds |
| topic | Liquid chromatography Isotopologues Retention mechanism Separation selectivity Isotope effects |
| url | http://www.sciencedirect.com/science/article/pii/S2772391725000052 |
| work_keys_str_mv | AT pnnesterenko tutorialonhighperformanceliquidchromatographyofisotopologuesoforganiccompounds AT vdkazakova tutorialonhighperformanceliquidchromatographyofisotopologuesoforganiccompounds |