Perforin Expression in Peripheral Blood Lymphatic Cells of Patients Subjected to Laparoscopic or Open Cholecystectomy
Perforin-(P-) related characteristics of cytotoxic T lymphocytes and natural killer cells were investigated in peripheral blood of patients subjected to open (OC; n=23) or laparoscopic cholecystectomy (LC; n=21) and healthy controls (n=20). Blood samples were obtained preoperatively and 24 hours aft...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2009/125152 |
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| author | Harry Grbas Ines Mrakovčić-Šutić Arsen Depolo Biserka Radošević-Stašić |
| author_facet | Harry Grbas Ines Mrakovčić-Šutić Arsen Depolo Biserka Radošević-Stašić |
| author_sort | Harry Grbas |
| collection | DOAJ |
| description | Perforin-(P-) related characteristics of cytotoxic T lymphocytes and natural killer cells were investigated in peripheral blood of patients subjected to open (OC; n=23) or laparoscopic cholecystectomy (LC; n=21) and healthy controls (n=20). Blood samples were obtained preoperatively and 24 hours after the surgeries, and the data were correlated with the intensity of cholestasis and concomitant inflammation, determined by functional hepatic tests.
Postoperative differences were found to be minimal: OC decreased only the percentage of CD56+ cells, while LC decreased the fraction of CD8+P+ cells and augmented the mean fluorescence intensity of P in CD56 cells. Patients elected for OC had, however, higher preoperative numbers of total P+, CD3+P+, and CD4+P+ cells than patients elected for LC and healthy controls, while both groups of patients, preoperatively, had lower fraction of CD16+P+ and CD56+P+ cells. These changes were in high correlation with blood concentrations of CRP, AP, and ALT, emphasizing the link between the preoperative cholestasis and inflammation and P-dependent cytotoxic mechanisms. |
| format | Article |
| id | doaj-art-8b038099bdba49a7b40d4d5a604f88cb |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
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| series | Mediators of Inflammation |
| spelling | doaj-art-8b038099bdba49a7b40d4d5a604f88cb2025-08-20T02:09:26ZengWileyMediators of Inflammation0962-93511466-18612009-01-01200910.1155/2009/125152125152Perforin Expression in Peripheral Blood Lymphatic Cells of Patients Subjected to Laparoscopic or Open CholecystectomyHarry Grbas0Ines Mrakovčić-Šutić1Arsen Depolo2Biserka Radošević-Stašić3Department of Surgery, Clinical Hospital Center, Medical Faculty, University of Rijeka, 51000 Rijeka, CroatiaDepartment of Physiology and Immunology, Medical Faculty, University of Rijeka, 51000 Rijeka, CroatiaDepartment of Surgery, Clinical Hospital Center, Medical Faculty, University of Rijeka, 51000 Rijeka, CroatiaDepartment of Physiology and Immunology, Medical Faculty, University of Rijeka, 51000 Rijeka, CroatiaPerforin-(P-) related characteristics of cytotoxic T lymphocytes and natural killer cells were investigated in peripheral blood of patients subjected to open (OC; n=23) or laparoscopic cholecystectomy (LC; n=21) and healthy controls (n=20). Blood samples were obtained preoperatively and 24 hours after the surgeries, and the data were correlated with the intensity of cholestasis and concomitant inflammation, determined by functional hepatic tests. Postoperative differences were found to be minimal: OC decreased only the percentage of CD56+ cells, while LC decreased the fraction of CD8+P+ cells and augmented the mean fluorescence intensity of P in CD56 cells. Patients elected for OC had, however, higher preoperative numbers of total P+, CD3+P+, and CD4+P+ cells than patients elected for LC and healthy controls, while both groups of patients, preoperatively, had lower fraction of CD16+P+ and CD56+P+ cells. These changes were in high correlation with blood concentrations of CRP, AP, and ALT, emphasizing the link between the preoperative cholestasis and inflammation and P-dependent cytotoxic mechanisms.http://dx.doi.org/10.1155/2009/125152 |
| spellingShingle | Harry Grbas Ines Mrakovčić-Šutić Arsen Depolo Biserka Radošević-Stašić Perforin Expression in Peripheral Blood Lymphatic Cells of Patients Subjected to Laparoscopic or Open Cholecystectomy Mediators of Inflammation |
| title | Perforin Expression in Peripheral Blood Lymphatic Cells of Patients Subjected to Laparoscopic or Open Cholecystectomy |
| title_full | Perforin Expression in Peripheral Blood Lymphatic Cells of Patients Subjected to Laparoscopic or Open Cholecystectomy |
| title_fullStr | Perforin Expression in Peripheral Blood Lymphatic Cells of Patients Subjected to Laparoscopic or Open Cholecystectomy |
| title_full_unstemmed | Perforin Expression in Peripheral Blood Lymphatic Cells of Patients Subjected to Laparoscopic or Open Cholecystectomy |
| title_short | Perforin Expression in Peripheral Blood Lymphatic Cells of Patients Subjected to Laparoscopic or Open Cholecystectomy |
| title_sort | perforin expression in peripheral blood lymphatic cells of patients subjected to laparoscopic or open cholecystectomy |
| url | http://dx.doi.org/10.1155/2009/125152 |
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