Studies on extracellular metabolites of two endophytic and epiphytic algae of Heveochlorella spp
To investigate the differences of extracellular metabolites between endophytic algae of Heveochlorella hainangensis and epiphytic algae of Heveochlorella roystonensis, the biomass and pH of culture medium were analyzed, as well as the extracellular metabolites were qualitatively detected by non-targ...
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| Language: | English |
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Zhejiang University Press
2021-06-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2020.09.221 |
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| author | DENG Ruru MA Shuai TAN Deguan FU Lili ZHANG Jiaming SUN Xuepiao LIU Pinghuai |
| author_facet | DENG Ruru MA Shuai TAN Deguan FU Lili ZHANG Jiaming SUN Xuepiao LIU Pinghuai |
| author_sort | DENG Ruru |
| collection | DOAJ |
| description | To investigate the differences of extracellular metabolites between endophytic algae of Heveochlorella hainangensis and epiphytic algae of Heveochlorella roystonensis, the biomass and pH of culture medium were analyzed, as well as the extracellular metabolites were qualitatively detected by non-targeted metabolomics analysis. The results showed that totally 141 compounds were detected in the culture media of the two algae species, of which eight and six ingredients specifically presented in the culture media of H. hainangensis and H. roystonensis, respectively. The relative contents of amino acids and their derivatives, ketone alcohols and other components in their extracellular metabolites were similar, but those of organic acids were remarkably different. After cultured for 15 d in the culture medium without exogenous organic carbon sources, the growth of H. hainangensis was slow, and the culture medium was slightly acidic, and the proportion of organic acids was 3.28%. However, the growth of H. roystonensis was normal, and the culture medium was alkaline, and the proportion of organic acids was 2.04%. When cultured in the media containing 0.5% sucrose for 15 d, the biomass of H. hainangensis increased from 0.09 g/L to 3.87 g/L; the proportion of organic acids increased to 4.64%; the contents of amines and alkaloids decreased; and the pH of culture medium decreased to 4.70. In the meantime, the biomass of H. roystonensis increased from 3.20 g/L to 5.24 g/L; the proportion of organic acids decreased to 1.13%; the contents of amines and alkaloids increased; and the pH of culture medium increased to 8.32. In conclusion, the change of pH in the culture media of the two species is opposite. The addition of organic carbon sources could significantly promote their growth and reproduction, promote the secretion of organic acids from H. hainangensis, inhibit the secretion of organic acids from H. roystonensis, thus enhance the degree of acidification or alkalization of the two cultures. The above results can provide a basis for the research on the evolution of Heveochlorella and the development and utilization of extracellular metabolites. |
| format | Article |
| id | doaj-art-8aff1929a69f4f299bca7d20adc95066 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2021-06-01 |
| publisher | Zhejiang University Press |
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| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-8aff1929a69f4f299bca7d20adc950662025-08-20T03:32:05ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552021-06-014736337010.3785/j.issn.1008-9209.2020.09.22110089209Studies on extracellular metabolites of two endophytic and epiphytic algae of Heveochlorella sppDENG RuruMA ShuaiTAN DeguanFU LiliZHANG JiamingSUN XuepiaoLIU PinghuaiTo investigate the differences of extracellular metabolites between endophytic algae of Heveochlorella hainangensis and epiphytic algae of Heveochlorella roystonensis, the biomass and pH of culture medium were analyzed, as well as the extracellular metabolites were qualitatively detected by non-targeted metabolomics analysis. The results showed that totally 141 compounds were detected in the culture media of the two algae species, of which eight and six ingredients specifically presented in the culture media of H. hainangensis and H. roystonensis, respectively. The relative contents of amino acids and their derivatives, ketone alcohols and other components in their extracellular metabolites were similar, but those of organic acids were remarkably different. After cultured for 15 d in the culture medium without exogenous organic carbon sources, the growth of H. hainangensis was slow, and the culture medium was slightly acidic, and the proportion of organic acids was 3.28%. However, the growth of H. roystonensis was normal, and the culture medium was alkaline, and the proportion of organic acids was 2.04%. When cultured in the media containing 0.5% sucrose for 15 d, the biomass of H. hainangensis increased from 0.09 g/L to 3.87 g/L; the proportion of organic acids increased to 4.64%; the contents of amines and alkaloids decreased; and the pH of culture medium decreased to 4.70. In the meantime, the biomass of H. roystonensis increased from 3.20 g/L to 5.24 g/L; the proportion of organic acids decreased to 1.13%; the contents of amines and alkaloids increased; and the pH of culture medium increased to 8.32. In conclusion, the change of pH in the culture media of the two species is opposite. The addition of organic carbon sources could significantly promote their growth and reproduction, promote the secretion of organic acids from H. hainangensis, inhibit the secretion of organic acids from H. roystonensis, thus enhance the degree of acidification or alkalization of the two cultures. The above results can provide a basis for the research on the evolution of Heveochlorella and the development and utilization of extracellular metabolites.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2020.09.221<italic>Heveochlorella hainanensis</italic> <italic>Heveochlorella roystonensis</italic> extracellular metabolitesnon-targeted metabolomics |
| spellingShingle | DENG Ruru MA Shuai TAN Deguan FU Lili ZHANG Jiaming SUN Xuepiao LIU Pinghuai Studies on extracellular metabolites of two endophytic and epiphytic algae of Heveochlorella spp 浙江大学学报. 农业与生命科学版 <italic>Heveochlorella hainanensis</italic> <italic>Heveochlorella roystonensis</italic> extracellular metabolites non-targeted metabolomics |
| title | Studies on extracellular metabolites of two endophytic and epiphytic algae of Heveochlorella spp |
| title_full | Studies on extracellular metabolites of two endophytic and epiphytic algae of Heveochlorella spp |
| title_fullStr | Studies on extracellular metabolites of two endophytic and epiphytic algae of Heveochlorella spp |
| title_full_unstemmed | Studies on extracellular metabolites of two endophytic and epiphytic algae of Heveochlorella spp |
| title_short | Studies on extracellular metabolites of two endophytic and epiphytic algae of Heveochlorella spp |
| title_sort | studies on extracellular metabolites of two endophytic and epiphytic algae of heveochlorella spp |
| topic | <italic>Heveochlorella hainanensis</italic> <italic>Heveochlorella roystonensis</italic> extracellular metabolites non-targeted metabolomics |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2020.09.221 |
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