Detection of Hedyotis corymbosa (L.) Lam. in traditional Chinese medicine Hedyotis diffusa Willd. with the Proofman-LMTIA method
There is increasing demand for the traditional Chinese medicine Hedyotis diffusa Willd. due to its effectiveness in treating hepatitis and malignant tumors of the liver, lung, and stomach. Hedyotis corymbosa (L.) Lam. has commonly become an adulterant of H. diffusa Willd. The proofreading enzyme-med...
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2025-07-01
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| Series: | Pharmacia |
| Online Access: | https://pharmacia.pensoft.net/article/157723/download/pdf/ |
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| author | Yongzhen Wang Juan Tian Dan Zhou Deguo Wang Bailing Yin |
| author_facet | Yongzhen Wang Juan Tian Dan Zhou Deguo Wang Bailing Yin |
| author_sort | Yongzhen Wang |
| collection | DOAJ |
| description | There is increasing demand for the traditional Chinese medicine Hedyotis diffusa Willd. due to its effectiveness in treating hepatitis and malignant tumors of the liver, lung, and stomach. Hedyotis corymbosa (L.) Lam. has commonly become an adulterant of H. diffusa Willd. The proofreading enzyme-mediated probe cleavage coupled with ladder-shape melting temperature isothermal amplification (Proofman-LMTIA) can specifically and sensitively detect nucleic acids. In this study, the Proofman-LMTIA method was established to identify H. corymbosa (L.) Lam. for quality control of the traditional Chinese medicine Hedyotis diffusa Willd. The LMTIA primers and Proofman probe were designed using the internal transcribed spacer 2 (ITS2) of H. corymbosa (L.) Lam. as the target. The reaction temperature was optimized, and the specificity and sensitivity were determined. The repeatability and reliability were also assessed. The optimal reaction temperature of the Proofman-LMTIA method was 61 °C. The method demonstrated high specificity, repeatability, reliability, and sensitivity, with amplification completed within 20 min and a detection limit of 10 pg/μL genomic H. corymbosa (L.) Lam. This study successfully established a Proofman-LMTIA method for the detection of H. corymbosa (L.) Lam., providing an effective approach to distinguish authentic from adulterated traditional Chinese medicine. |
| format | Article |
| id | doaj-art-8afdb5d776cb48d4b27626a9f40bcd51 |
| institution | DOAJ |
| issn | 2603-557X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Pharmacia |
| spelling | doaj-art-8afdb5d776cb48d4b27626a9f40bcd512025-08-20T03:08:51ZengPensoft PublishersPharmacia2603-557X2025-07-01721910.3897/pharmacia.72.e157723157723Detection of Hedyotis corymbosa (L.) Lam. in traditional Chinese medicine Hedyotis diffusa Willd. with the Proofman-LMTIA methodYongzhen Wang0Juan Tian1Dan Zhou2Deguo Wang3Bailing Yin4Xuchang UniversityXuchang UniversityXuchang UniversityXuchang UniversityYuzhou Houshengtang Traditional Chinese Medicine Co., LtdThere is increasing demand for the traditional Chinese medicine Hedyotis diffusa Willd. due to its effectiveness in treating hepatitis and malignant tumors of the liver, lung, and stomach. Hedyotis corymbosa (L.) Lam. has commonly become an adulterant of H. diffusa Willd. The proofreading enzyme-mediated probe cleavage coupled with ladder-shape melting temperature isothermal amplification (Proofman-LMTIA) can specifically and sensitively detect nucleic acids. In this study, the Proofman-LMTIA method was established to identify H. corymbosa (L.) Lam. for quality control of the traditional Chinese medicine Hedyotis diffusa Willd. The LMTIA primers and Proofman probe were designed using the internal transcribed spacer 2 (ITS2) of H. corymbosa (L.) Lam. as the target. The reaction temperature was optimized, and the specificity and sensitivity were determined. The repeatability and reliability were also assessed. The optimal reaction temperature of the Proofman-LMTIA method was 61 °C. The method demonstrated high specificity, repeatability, reliability, and sensitivity, with amplification completed within 20 min and a detection limit of 10 pg/μL genomic H. corymbosa (L.) Lam. This study successfully established a Proofman-LMTIA method for the detection of H. corymbosa (L.) Lam., providing an effective approach to distinguish authentic from adulterated traditional Chinese medicine.https://pharmacia.pensoft.net/article/157723/download/pdf/ |
| spellingShingle | Yongzhen Wang Juan Tian Dan Zhou Deguo Wang Bailing Yin Detection of Hedyotis corymbosa (L.) Lam. in traditional Chinese medicine Hedyotis diffusa Willd. with the Proofman-LMTIA method Pharmacia |
| title | Detection of Hedyotis corymbosa (L.) Lam. in traditional Chinese medicine Hedyotis diffusa Willd. with the Proofman-LMTIA method |
| title_full | Detection of Hedyotis corymbosa (L.) Lam. in traditional Chinese medicine Hedyotis diffusa Willd. with the Proofman-LMTIA method |
| title_fullStr | Detection of Hedyotis corymbosa (L.) Lam. in traditional Chinese medicine Hedyotis diffusa Willd. with the Proofman-LMTIA method |
| title_full_unstemmed | Detection of Hedyotis corymbosa (L.) Lam. in traditional Chinese medicine Hedyotis diffusa Willd. with the Proofman-LMTIA method |
| title_short | Detection of Hedyotis corymbosa (L.) Lam. in traditional Chinese medicine Hedyotis diffusa Willd. with the Proofman-LMTIA method |
| title_sort | detection of hedyotis corymbosa l lam in traditional chinese medicine hedyotis diffusa willd with the proofman lmtia method |
| url | https://pharmacia.pensoft.net/article/157723/download/pdf/ |
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