EXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMA
Abstract. We studied expression of transcription factor T-bet protein in allergic (ABA) versus non-allergic bronchial asthma (NABA), depending on severity grade and clinical phase of the disease. A Western blotting technique was applied, according to the standard protocol (Amersham), using polyclona...
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
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| Series: | Медицинская иммунология |
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| Online Access: | https://www.mimmun.ru/mimmun/article/view/327 |
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| _version_ | 1849339359565709312 |
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| author | V. N. Mineev L. N. Sorokina |
| author_facet | V. N. Mineev L. N. Sorokina |
| author_sort | V. N. Mineev |
| collection | DOAJ |
| description | Abstract. We studied expression of transcription factor T-bet protein in allergic (ABA) versus non-allergic bronchial asthma (NABA), depending on severity grade and clinical phase of the disease. A Western blotting technique was applied, according to the standard protocol (Amersham), using polyclonal anti-T-bet antibodies at a 1:500 dilution (Santa Cruz Biotechnology, UK). The protein levels were quantified, using β-actin as a reference. It is shown for the first time that T-bet expression in ABA patients, as compared to healthy controls, was reduced with increasing disease severity, but did not correlate with clinical phase of the disease. In NABA patients, no sufficient differences in T-bet expression were revealed, as compared with healthy controls. The work was supported by St.Petersburg governmental grant PD04-4.0-102 (Certificate № ASP604079). |
| format | Article |
| id | doaj-art-8af325dc5bc24bb58d4839bb79a38b93 |
| institution | Kabale University |
| issn | 1563-0625 2313-741X |
| language | Russian |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj-art-8af325dc5bc24bb58d4839bb79a38b932025-08-20T03:44:08ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-01141-216917310.15789/1563-0625-2012-1-2-169-173324EXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMAV. N. Mineev0L. N. Sorokina1Санкт-Петербургский государственный медицинский университет имени академика И.П. Павлова, Санкт-ПетербургСанкт-Петербургский государственный медицинский университет имени академика И.П. Павлова, Санкт-ПетербургAbstract. We studied expression of transcription factor T-bet protein in allergic (ABA) versus non-allergic bronchial asthma (NABA), depending on severity grade and clinical phase of the disease. A Western blotting technique was applied, according to the standard protocol (Amersham), using polyclonal anti-T-bet antibodies at a 1:500 dilution (Santa Cruz Biotechnology, UK). The protein levels were quantified, using β-actin as a reference. It is shown for the first time that T-bet expression in ABA patients, as compared to healthy controls, was reduced with increasing disease severity, but did not correlate with clinical phase of the disease. In NABA patients, no sufficient differences in T-bet expression were revealed, as compared with healthy controls. The work was supported by St.Petersburg governmental grant PD04-4.0-102 (Certificate № ASP604079).https://www.mimmun.ru/mimmun/article/view/327bronchial asthmat-betmononuclear cells |
| spellingShingle | V. N. Mineev L. N. Sorokina EXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMA Медицинская иммунология bronchial asthma t-bet mononuclear cells |
| title | EXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMA |
| title_full | EXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMA |
| title_fullStr | EXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMA |
| title_full_unstemmed | EXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMA |
| title_short | EXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMA |
| title_sort | expression of t bet factor in peripheral blood mononuclear cells in bronchial asthma |
| topic | bronchial asthma t-bet mononuclear cells |
| url | https://www.mimmun.ru/mimmun/article/view/327 |
| work_keys_str_mv | AT vnmineev expressionoftbetfactorinperipheralbloodmononuclearcellsinbronchialasthma AT lnsorokina expressionoftbetfactorinperipheralbloodmononuclearcellsinbronchialasthma |