Identification and characterization of a novel QTL for barley yellow mosaic disease resistance from bulbous barley
Abstract Winter barley (Hordeum vulgare) production areas in the middle and lower reaches of the Yangtze River are severely threatened by barley yellow mosaic disease, which is caused by Barley yellow mosaic virus and Barley mild mosaic virus. Improving barley disease resistance in breeding programs...
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| Format: | Article |
| Language: | English |
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Wiley
2025-03-01
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| Series: | The Plant Genome |
| Online Access: | https://doi.org/10.1002/tpg2.20557 |
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| author | Yi Hong Hui Zhou Mengna Zhang Yuhang Zhang Juan Zhu Chao Lv Baojian Guo Feifei Wang Qingliang Li Jie Sun Rugen Xu |
| author_facet | Yi Hong Hui Zhou Mengna Zhang Yuhang Zhang Juan Zhu Chao Lv Baojian Guo Feifei Wang Qingliang Li Jie Sun Rugen Xu |
| author_sort | Yi Hong |
| collection | DOAJ |
| description | Abstract Winter barley (Hordeum vulgare) production areas in the middle and lower reaches of the Yangtze River are severely threatened by barley yellow mosaic disease, which is caused by Barley yellow mosaic virus and Barley mild mosaic virus. Improving barley disease resistance in breeding programs requires knowledge of genetic loci in germplasm resources. In this study, bulked segregant analysis (BSA) identified a novel major quantitative trait loci (QTL) QRym.ZN1‐7H for barley yellow mosaic disease resistance in an F2 population derived from the cross between “Nongke 1–6” (H. vulgare) and “Zaoshu 3” (H. vulgare). This QTL, originating from bulbous barley (Hordeum bulbosum), demonstrated stability and was further validated in another F2 population derived from the cross between “Nongke 2–6” (H. vulgare) and “Supi 1” (H. vulgare). QRym.ZN1‐7H accounted for 10.61%–19.34% of the phenotypic variance. The QTL was further fine mapped to the 14‐ to 39‐Mb interval on barley chromosome 7H. Transcriptome analysis identified 53 and 35 differentially expressed genes in roots and leaves (at QRym.ZN1‐7H locus), respectively, with nine genes differentially expressing in both tissues. HORVU.MOREX.r3.7HG0650990, a member of the disease resistance protein family (NBS‐LRR class), is the most likely candidate gene for QRym.ZN1‐7H. Enrichment analysis indicated that QRym.ZN1‐7H may be involved in signal transduction in plant innate immune response. This study laid a foundation for barley disease resistance breeding. |
| format | Article |
| id | doaj-art-8aec816940a6446faf8a3bc5d458bbb5 |
| institution | Kabale University |
| issn | 1940-3372 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Plant Genome |
| spelling | doaj-art-8aec816940a6446faf8a3bc5d458bbb52025-08-20T03:43:57ZengWileyThe Plant Genome1940-33722025-03-01181n/an/a10.1002/tpg2.20557Identification and characterization of a novel QTL for barley yellow mosaic disease resistance from bulbous barleyYi Hong0Hui Zhou1Mengna Zhang2Yuhang Zhang3Juan Zhu4Chao Lv5Baojian Guo6Feifei Wang7Qingliang Li8Jie Sun9Rugen Xu10Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co‐Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri‐Product Safety of Ministry of Education of China Yangzhou University Yangzhou ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co‐Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri‐Product Safety of Ministry of Education of China Yangzhou University Yangzhou ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co‐Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri‐Product Safety of Ministry of Education of China Yangzhou University Yangzhou ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co‐Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri‐Product Safety of Ministry of Education of China Yangzhou University Yangzhou ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co‐Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri‐Product Safety of Ministry of Education of China Yangzhou University Yangzhou ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co‐Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri‐Product Safety of Ministry of Education of China Yangzhou University Yangzhou ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co‐Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri‐Product Safety of Ministry of Education of China Yangzhou University Yangzhou ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co‐Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri‐Product Safety of Ministry of Education of China Yangzhou University Yangzhou ChinaChina Resources Beer (Holdings) Company Limited Beijing ChinaChina Resources Beer (Holdings) Company Limited Beijing ChinaKey Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co‐Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture and Agri‐Product Safety of Ministry of Education of China Yangzhou University Yangzhou ChinaAbstract Winter barley (Hordeum vulgare) production areas in the middle and lower reaches of the Yangtze River are severely threatened by barley yellow mosaic disease, which is caused by Barley yellow mosaic virus and Barley mild mosaic virus. Improving barley disease resistance in breeding programs requires knowledge of genetic loci in germplasm resources. In this study, bulked segregant analysis (BSA) identified a novel major quantitative trait loci (QTL) QRym.ZN1‐7H for barley yellow mosaic disease resistance in an F2 population derived from the cross between “Nongke 1–6” (H. vulgare) and “Zaoshu 3” (H. vulgare). This QTL, originating from bulbous barley (Hordeum bulbosum), demonstrated stability and was further validated in another F2 population derived from the cross between “Nongke 2–6” (H. vulgare) and “Supi 1” (H. vulgare). QRym.ZN1‐7H accounted for 10.61%–19.34% of the phenotypic variance. The QTL was further fine mapped to the 14‐ to 39‐Mb interval on barley chromosome 7H. Transcriptome analysis identified 53 and 35 differentially expressed genes in roots and leaves (at QRym.ZN1‐7H locus), respectively, with nine genes differentially expressing in both tissues. HORVU.MOREX.r3.7HG0650990, a member of the disease resistance protein family (NBS‐LRR class), is the most likely candidate gene for QRym.ZN1‐7H. Enrichment analysis indicated that QRym.ZN1‐7H may be involved in signal transduction in plant innate immune response. This study laid a foundation for barley disease resistance breeding.https://doi.org/10.1002/tpg2.20557 |
| spellingShingle | Yi Hong Hui Zhou Mengna Zhang Yuhang Zhang Juan Zhu Chao Lv Baojian Guo Feifei Wang Qingliang Li Jie Sun Rugen Xu Identification and characterization of a novel QTL for barley yellow mosaic disease resistance from bulbous barley The Plant Genome |
| title | Identification and characterization of a novel QTL for barley yellow mosaic disease resistance from bulbous barley |
| title_full | Identification and characterization of a novel QTL for barley yellow mosaic disease resistance from bulbous barley |
| title_fullStr | Identification and characterization of a novel QTL for barley yellow mosaic disease resistance from bulbous barley |
| title_full_unstemmed | Identification and characterization of a novel QTL for barley yellow mosaic disease resistance from bulbous barley |
| title_short | Identification and characterization of a novel QTL for barley yellow mosaic disease resistance from bulbous barley |
| title_sort | identification and characterization of a novel qtl for barley yellow mosaic disease resistance from bulbous barley |
| url | https://doi.org/10.1002/tpg2.20557 |
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