CX3CR1 knockout aggravates Coxsackievirus B3-induced myocarditis.
Studies on inflammatory disorders elucidated the pivotal role of the CX3CL1/CX3CR1 axis with respect to the pathophysiology and diseases progression. Coxsackievirus B3 (CVB3)-induced myocarditis is associated with severe cardiac inflammation, which may progress to heart failure. We therefore investi...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182643&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850076499849248768 |
|---|---|
| author | Irene Müller Kathleen Pappritz Konstantinos Savvatis Kerstin Puhl Fengquan Dong Muhammad El-Shafeey Nazha Hamdani Isabell Hamann Michel Noutsias Carmen Infante-Duarte Wolfgang A Linke Sophie Van Linthout Carsten Tschöpe |
| author_facet | Irene Müller Kathleen Pappritz Konstantinos Savvatis Kerstin Puhl Fengquan Dong Muhammad El-Shafeey Nazha Hamdani Isabell Hamann Michel Noutsias Carmen Infante-Duarte Wolfgang A Linke Sophie Van Linthout Carsten Tschöpe |
| author_sort | Irene Müller |
| collection | DOAJ |
| description | Studies on inflammatory disorders elucidated the pivotal role of the CX3CL1/CX3CR1 axis with respect to the pathophysiology and diseases progression. Coxsackievirus B3 (CVB3)-induced myocarditis is associated with severe cardiac inflammation, which may progress to heart failure. We therefore investigated the influence of CX3CR1 ablation in the model of acute myocarditis, which was induced by inoculation with 5x105 plaque forming units of CVB3 (Nancy strain) in either CX3CR1-/- or C57BL6/j (WT) mice. Seven days after infection, myocardial inflammation, remodeling, and titin expression and phosphorylation were examined by immunohistochemistry, real-time PCR and Pro-Q diamond stain. Cardiac function was assessed by tip catheter. Compared to WT CVB3 mice, CX3CR1-/- CVB3 mice exhibited enhanced left ventricular expression of inflammatory cytokines and chemokines, which was associated with an increase of immune cell infiltration/presence. This shift towards a pro-inflammatory immune response further resulted in increased cardiac fibrosis and cardiomyocyte apoptosis, which was reflected by an impaired cardiac function in CX3CR1-/- CVB3 compared to WT CVB3 mice. These findings demonstrate a cardioprotective role of CX3CR1 in CVB3-infected mice and indicate the relevance of the CX3CL1/CX3CR1 system in CVB3-induced myocarditis. |
| format | Article |
| id | doaj-art-8adfa9f2ae684b97a588dc84787a2dc8 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-8adfa9f2ae684b97a588dc84787a2dc82025-08-20T02:46:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018264310.1371/journal.pone.0182643CX3CR1 knockout aggravates Coxsackievirus B3-induced myocarditis.Irene MüllerKathleen PappritzKonstantinos SavvatisKerstin PuhlFengquan DongMuhammad El-ShafeeyNazha HamdaniIsabell HamannMichel NoutsiasCarmen Infante-DuarteWolfgang A LinkeSophie Van LinthoutCarsten TschöpeStudies on inflammatory disorders elucidated the pivotal role of the CX3CL1/CX3CR1 axis with respect to the pathophysiology and diseases progression. Coxsackievirus B3 (CVB3)-induced myocarditis is associated with severe cardiac inflammation, which may progress to heart failure. We therefore investigated the influence of CX3CR1 ablation in the model of acute myocarditis, which was induced by inoculation with 5x105 plaque forming units of CVB3 (Nancy strain) in either CX3CR1-/- or C57BL6/j (WT) mice. Seven days after infection, myocardial inflammation, remodeling, and titin expression and phosphorylation were examined by immunohistochemistry, real-time PCR and Pro-Q diamond stain. Cardiac function was assessed by tip catheter. Compared to WT CVB3 mice, CX3CR1-/- CVB3 mice exhibited enhanced left ventricular expression of inflammatory cytokines and chemokines, which was associated with an increase of immune cell infiltration/presence. This shift towards a pro-inflammatory immune response further resulted in increased cardiac fibrosis and cardiomyocyte apoptosis, which was reflected by an impaired cardiac function in CX3CR1-/- CVB3 compared to WT CVB3 mice. These findings demonstrate a cardioprotective role of CX3CR1 in CVB3-infected mice and indicate the relevance of the CX3CL1/CX3CR1 system in CVB3-induced myocarditis.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182643&type=printable |
| spellingShingle | Irene Müller Kathleen Pappritz Konstantinos Savvatis Kerstin Puhl Fengquan Dong Muhammad El-Shafeey Nazha Hamdani Isabell Hamann Michel Noutsias Carmen Infante-Duarte Wolfgang A Linke Sophie Van Linthout Carsten Tschöpe CX3CR1 knockout aggravates Coxsackievirus B3-induced myocarditis. PLoS ONE |
| title | CX3CR1 knockout aggravates Coxsackievirus B3-induced myocarditis. |
| title_full | CX3CR1 knockout aggravates Coxsackievirus B3-induced myocarditis. |
| title_fullStr | CX3CR1 knockout aggravates Coxsackievirus B3-induced myocarditis. |
| title_full_unstemmed | CX3CR1 knockout aggravates Coxsackievirus B3-induced myocarditis. |
| title_short | CX3CR1 knockout aggravates Coxsackievirus B3-induced myocarditis. |
| title_sort | cx3cr1 knockout aggravates coxsackievirus b3 induced myocarditis |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182643&type=printable |
| work_keys_str_mv | AT irenemuller cx3cr1knockoutaggravatescoxsackievirusb3inducedmyocarditis AT kathleenpappritz cx3cr1knockoutaggravatescoxsackievirusb3inducedmyocarditis AT konstantinossavvatis cx3cr1knockoutaggravatescoxsackievirusb3inducedmyocarditis AT kerstinpuhl cx3cr1knockoutaggravatescoxsackievirusb3inducedmyocarditis AT fengquandong cx3cr1knockoutaggravatescoxsackievirusb3inducedmyocarditis AT muhammadelshafeey cx3cr1knockoutaggravatescoxsackievirusb3inducedmyocarditis AT nazhahamdani cx3cr1knockoutaggravatescoxsackievirusb3inducedmyocarditis AT isabellhamann cx3cr1knockoutaggravatescoxsackievirusb3inducedmyocarditis AT michelnoutsias cx3cr1knockoutaggravatescoxsackievirusb3inducedmyocarditis AT carmeninfanteduarte cx3cr1knockoutaggravatescoxsackievirusb3inducedmyocarditis AT wolfgangalinke cx3cr1knockoutaggravatescoxsackievirusb3inducedmyocarditis AT sophievanlinthout cx3cr1knockoutaggravatescoxsackievirusb3inducedmyocarditis AT carstentschope cx3cr1knockoutaggravatescoxsackievirusb3inducedmyocarditis |