Efficient modification and preparation of circular DNA for expression in cell culture

Abstract DNA plasmids are an essential tool for delivery and expression of RNAs and proteins in cell culture experiments. The preparation of plasmids typically involves a laborious process of bacterial cloning, validation, and purification. While the expression plasmids can be designed and ordered f...

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Bibliographic Details
Main Authors: Roman Teo Oliynyk, George M. Church
Format: Article
Language:English
Published: Nature Portfolio 2022-12-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-022-04363-z
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