Efficient modification and preparation of circular DNA for expression in cell culture
Abstract DNA plasmids are an essential tool for delivery and expression of RNAs and proteins in cell culture experiments. The preparation of plasmids typically involves a laborious process of bacterial cloning, validation, and purification. While the expression plasmids can be designed and ordered f...
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| Main Authors: | Roman Teo Oliynyk, George M. Church |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-12-01
|
| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-022-04363-z |
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