Evaluation of a Burkholderia ambifaria strain from plants as a novel promising probiotic in dental caries management
Background Probiotics serve as a novel preventive or therapeutic approach for dental caries owing to their ability to reverse dysbiosis and restore a healthy microbiota. Here, we identified Burkholderia ambifaria AFS098024 as a probiotic candidate isolated from plants.Methods The safety of B. ambifa...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2024.2420612 |
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| author | Zirang Chen Yangyu Lu Zhezhen Xu Lijing Wu Xi Wei Yanling Cai |
| author_facet | Zirang Chen Yangyu Lu Zhezhen Xu Lijing Wu Xi Wei Yanling Cai |
| author_sort | Zirang Chen |
| collection | DOAJ |
| description | Background Probiotics serve as a novel preventive or therapeutic approach for dental caries owing to their ability to reverse dysbiosis and restore a healthy microbiota. Here, we identified Burkholderia ambifaria AFS098024 as a probiotic candidate isolated from plants.Methods The safety of B. ambifaria was evaluated by hemolytic activity, D-lactic acid production and antibiotic susceptibility. In vitro biofilm model derived from the saliva of caries-free and caries-active donors and in vivo rat caries model were used to assess the efficacy of B. ambifaria in caries prevention and treatment.Results B. ambifaria was safe as a probiotic candidate and it could integrate with in vitro biofilm model. It significantly reduced the biomass and lactate production of biofilms from caries-active donors and disrupted biofilm structures. B. ambifaria effectively reduced the severity of carious lesions in rat molars, regardless of the inoculation sequence. Molars pretreated or treated with B. ambifaria demonstrated notably higher enamel volumes. Additionally, colonization of rat molars by B. ambifaria persisted for 6 weeks.Conclusion The B. ambifaria strain used in this study holds promise as a probiotic for inhibiting dental caries, both in vitro and in vivo. |
| format | Article |
| id | doaj-art-8acb2ab496c0471789bdd3ad306e7ce8 |
| institution | OA Journals |
| issn | 2000-2297 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj-art-8acb2ab496c0471789bdd3ad306e7ce82025-08-20T01:53:15ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972024-12-0116110.1080/20002297.2024.2420612Evaluation of a Burkholderia ambifaria strain from plants as a novel promising probiotic in dental caries managementZirang Chen0Yangyu Lu1Zhezhen Xu2Lijing Wu3Xi Wei4Yanling Cai5Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaHospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaHospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaHospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaHospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaHospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, ChinaBackground Probiotics serve as a novel preventive or therapeutic approach for dental caries owing to their ability to reverse dysbiosis and restore a healthy microbiota. Here, we identified Burkholderia ambifaria AFS098024 as a probiotic candidate isolated from plants.Methods The safety of B. ambifaria was evaluated by hemolytic activity, D-lactic acid production and antibiotic susceptibility. In vitro biofilm model derived from the saliva of caries-free and caries-active donors and in vivo rat caries model were used to assess the efficacy of B. ambifaria in caries prevention and treatment.Results B. ambifaria was safe as a probiotic candidate and it could integrate with in vitro biofilm model. It significantly reduced the biomass and lactate production of biofilms from caries-active donors and disrupted biofilm structures. B. ambifaria effectively reduced the severity of carious lesions in rat molars, regardless of the inoculation sequence. Molars pretreated or treated with B. ambifaria demonstrated notably higher enamel volumes. Additionally, colonization of rat molars by B. ambifaria persisted for 6 weeks.Conclusion The B. ambifaria strain used in this study holds promise as a probiotic for inhibiting dental caries, both in vitro and in vivo.https://www.tandfonline.com/doi/10.1080/20002297.2024.2420612Burkholderia ambifariaplantsprobioticsdental cariessaliva-derived biofilmsrat caries model |
| spellingShingle | Zirang Chen Yangyu Lu Zhezhen Xu Lijing Wu Xi Wei Yanling Cai Evaluation of a Burkholderia ambifaria strain from plants as a novel promising probiotic in dental caries management Journal of Oral Microbiology Burkholderia ambifaria plants probiotics dental caries saliva-derived biofilms rat caries model |
| title | Evaluation of a Burkholderia ambifaria strain from plants as a novel promising probiotic in dental caries management |
| title_full | Evaluation of a Burkholderia ambifaria strain from plants as a novel promising probiotic in dental caries management |
| title_fullStr | Evaluation of a Burkholderia ambifaria strain from plants as a novel promising probiotic in dental caries management |
| title_full_unstemmed | Evaluation of a Burkholderia ambifaria strain from plants as a novel promising probiotic in dental caries management |
| title_short | Evaluation of a Burkholderia ambifaria strain from plants as a novel promising probiotic in dental caries management |
| title_sort | evaluation of a burkholderia ambifaria strain from plants as a novel promising probiotic in dental caries management |
| topic | Burkholderia ambifaria plants probiotics dental caries saliva-derived biofilms rat caries model |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2024.2420612 |
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