Occurrence and antimicrobial susceptibility of Salmonella isolates recovered from the pig slaughter process in Romania

Introduction: Reported human salmonellosis cases have increased in Romania. Antibiotic susceptibility testing of Salmonella strains isolated from pork and chicken meat indicate a worrying multidrug resistance pattern. This study aimed to investigate the occurrence of Salmonella and to evaluate the a...

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Main Authors: Adriana Morar, Claudia Sala, Kálmán Imre
Format: Article
Language:English
Published: The Journal of Infection in Developing Countries 2015-01-01
Series:Journal of Infection in Developing Countries
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Online Access:https://jidc.org/index.php/journal/article/view/5236
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author Adriana Morar
Claudia Sala
Kálmán Imre
author_facet Adriana Morar
Claudia Sala
Kálmán Imre
author_sort Adriana Morar
collection DOAJ
description Introduction: Reported human salmonellosis cases have increased in Romania. Antibiotic susceptibility testing of Salmonella strains isolated from pork and chicken meat indicate a worrying multidrug resistance pattern. This study aimed to investigate the occurrence of Salmonella and to evaluate the antibiotic resistance of Salmonella strains in a pig slaughterhouse-processing complex, which receives animals from 30% of the large industrialized swine farms in Romania. Methodology: A total of 108 samples, including pork (n = 47), packaged pork products (n = 44), scald water sludge (n = 8), and detritus from the hair removal machine of the slaughterhouse (n = 9) were examined for the presence of Salmonella through standard methods. The antibiotic susceptibility of the isolated strains to 17 antibiotics was tested using the Vitek 2 system. Results: Twenty-six (24.1%) samples were found to be Salmonella positive; this included 25.5% of meat samples and 15.9% of packaged products, as well as samples from two different points of the slaughter (41.2%). Resistance was observed against tetracycline (61.5%), ampicillin (50%), piperacillin (50%), trimethoprim-sulfamethoxazole (34.6%), amoxicillin/clavulanic acid (26.9%), nitrofurantion (23.1%), cefazolin (15.4%), piperacillin/tazobactam (7.7%), imipenem (3.8%), ciprofloxacin (3.8%), and norfloxacin (3.8%). No resistance towards cefoxitin, cefotaxime, ceftazidime, cefepime, amikacin, and gentamicin was found. Conclusions: Our study demonstrated the occurrence of multidrug-resistant Salmonella strains in the investigated pork production complex and highlighted it as a potential source of human infections. The results demonstrate the seriousness of antibiotic resistance of Salmonella in Romania, while providing a useful insight for the treatment of human salmonellosis by specialists.
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spelling doaj-art-8ac8983d030a4fe9be776aebe660b06c2025-08-20T03:52:42ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802015-01-0190110.3855/jidc.5236Occurrence and antimicrobial susceptibility of Salmonella isolates recovered from the pig slaughter process in RomaniaAdriana Morar0Claudia Sala1Kálmán Imre2Banat's University of Agricultural Sciences and Veterinary Medicine King Michael I of Romania, Timişoara, RomaniaBanat's University of Agricultural Sciences and Veterinary Medicine King Michael I of Romania, Timişoara, RomaniaBanat's University of Agricultural Sciences and Veterinary Medicine King Michael I of Romania, Timişoara, RomaniaIntroduction: Reported human salmonellosis cases have increased in Romania. Antibiotic susceptibility testing of Salmonella strains isolated from pork and chicken meat indicate a worrying multidrug resistance pattern. This study aimed to investigate the occurrence of Salmonella and to evaluate the antibiotic resistance of Salmonella strains in a pig slaughterhouse-processing complex, which receives animals from 30% of the large industrialized swine farms in Romania. Methodology: A total of 108 samples, including pork (n = 47), packaged pork products (n = 44), scald water sludge (n = 8), and detritus from the hair removal machine of the slaughterhouse (n = 9) were examined for the presence of Salmonella through standard methods. The antibiotic susceptibility of the isolated strains to 17 antibiotics was tested using the Vitek 2 system. Results: Twenty-six (24.1%) samples were found to be Salmonella positive; this included 25.5% of meat samples and 15.9% of packaged products, as well as samples from two different points of the slaughter (41.2%). Resistance was observed against tetracycline (61.5%), ampicillin (50%), piperacillin (50%), trimethoprim-sulfamethoxazole (34.6%), amoxicillin/clavulanic acid (26.9%), nitrofurantion (23.1%), cefazolin (15.4%), piperacillin/tazobactam (7.7%), imipenem (3.8%), ciprofloxacin (3.8%), and norfloxacin (3.8%). No resistance towards cefoxitin, cefotaxime, ceftazidime, cefepime, amikacin, and gentamicin was found. Conclusions: Our study demonstrated the occurrence of multidrug-resistant Salmonella strains in the investigated pork production complex and highlighted it as a potential source of human infections. The results demonstrate the seriousness of antibiotic resistance of Salmonella in Romania, while providing a useful insight for the treatment of human salmonellosis by specialists. https://jidc.org/index.php/journal/article/view/5236Salmonellaslaughterhousepig meatmultidrug resistance
spellingShingle Adriana Morar
Claudia Sala
Kálmán Imre
Occurrence and antimicrobial susceptibility of Salmonella isolates recovered from the pig slaughter process in Romania
Journal of Infection in Developing Countries
Salmonella
slaughterhouse
pig meat
multidrug resistance
title Occurrence and antimicrobial susceptibility of Salmonella isolates recovered from the pig slaughter process in Romania
title_full Occurrence and antimicrobial susceptibility of Salmonella isolates recovered from the pig slaughter process in Romania
title_fullStr Occurrence and antimicrobial susceptibility of Salmonella isolates recovered from the pig slaughter process in Romania
title_full_unstemmed Occurrence and antimicrobial susceptibility of Salmonella isolates recovered from the pig slaughter process in Romania
title_short Occurrence and antimicrobial susceptibility of Salmonella isolates recovered from the pig slaughter process in Romania
title_sort occurrence and antimicrobial susceptibility of salmonella isolates recovered from the pig slaughter process in romania
topic Salmonella
slaughterhouse
pig meat
multidrug resistance
url https://jidc.org/index.php/journal/article/view/5236
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AT claudiasala occurrenceandantimicrobialsusceptibilityofsalmonellaisolatesrecoveredfromthepigslaughterprocessinromania
AT kalmanimre occurrenceandantimicrobialsusceptibilityofsalmonellaisolatesrecoveredfromthepigslaughterprocessinromania