Seven-Year Analysis of Microbial Keratitis Tendency at an Ophthalmology Department in Poland: A Single-Center Study
This study aimed to analyze the frequency, drug susceptibility, and drug resistance of pathogens causing microbial keratitis (a corneal inflammation) in the Clinical Department of Ophthalmology, Medical University of Silesia, Katowice. Despite intensive treatment, severe inflammation causes irrevers...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2020/8851570 |
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| author | Klaudia Ulfik Sławomir Teper Michał Dembski Anna Nowińska Ewa Wróblewska-Czajka Edward Wylęgała |
| author_facet | Klaudia Ulfik Sławomir Teper Michał Dembski Anna Nowińska Ewa Wróblewska-Czajka Edward Wylęgała |
| author_sort | Klaudia Ulfik |
| collection | DOAJ |
| description | This study aimed to analyze the frequency, drug susceptibility, and drug resistance of pathogens causing microbial keratitis (a corneal inflammation) in the Clinical Department of Ophthalmology, Medical University of Silesia, Katowice. Despite intensive treatment, severe inflammation causes irreversible blindness in ∼7% of cases and eye loss (evisceration or enucleation of the eyeball) in ∼1% of cases at our hospital. The choice of a targeted drug depends on the culture result and drug resistance of the microorganism. This was a retrospective observation study. Conjunctival swabs and corneal scrapes were collected between January 1, 2013, and December 31, 2019, in the tertiary reference center for keratitis. The collected data included the type of material received, culture result, and antimicrobial susceptibilities. Of the 2482 samples analyzed, 679 were positive and 1803 were negative. Of the total pathogens isolated, 69.9% were Gram-positive bacteria, 20.8% were Gram-negative bacteria, and 7.1% were fungi. A significant increase in the number of Gram-positive methicillin-resistant Staphylococcus aureus and a partial increase in the number of Gram-negative beta-lactams-resistant bacteria were observed. All fungal species were sensitive to amphotericin B, 82.81% were sensitive to voriconazole, and 56.25% were sensitive to fluconazole. Dual drug therapy (levofloxacin and tobramycin) was the first-line treatment. Drug susceptibility testing of the cultured microorganisms is necessary to initiate targeted treatment. Increased drug resistance was observed in this study. In the present study, most bacteria were sensitive to fluoroquinolones. Ciprofloxacin therapy remains the recommended empirical treatment in microbial keratitis. According to our study, voriconazole remains a first-line antifungal drug, when a fungal infection is suspected. |
| format | Article |
| id | doaj-art-8ac73c01ef304643b8899c220b2210ad |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-8ac73c01ef304643b8899c220b2210ad2025-02-03T06:45:51ZengWileyJournal of Ophthalmology2090-004X2090-00582020-01-01202010.1155/2020/88515708851570Seven-Year Analysis of Microbial Keratitis Tendency at an Ophthalmology Department in Poland: A Single-Center StudyKlaudia Ulfik0Sławomir Teper1Michał Dembski2Anna Nowińska3Ewa Wróblewska-Czajka4Edward Wylęgała5Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Chair and Department of Ophthalmology, Panewnicka 65, 40-760 Katowice, PolandFaculty of Medical Sciences in Zabrze, Medical University of Silesia, Chair and Department of Ophthalmology, Panewnicka 65, 40-760 Katowice, PolandFaculty of Medical Sciences in Zabrze, Medical University of Silesia, Chair and Department of Ophthalmology, Panewnicka 65, 40-760 Katowice, PolandFaculty of Medical Sciences in Zabrze, Medical University of Silesia, Chair and Department of Ophthalmology, Panewnicka 65, 40-760 Katowice, PolandFaculty of Medical Sciences in Zabrze, Medical University of Silesia, Chair and Department of Ophthalmology, Panewnicka 65, 40-760 Katowice, PolandFaculty of Medical Sciences in Zabrze, Medical University of Silesia, Chair and Department of Ophthalmology, Panewnicka 65, 40-760 Katowice, PolandThis study aimed to analyze the frequency, drug susceptibility, and drug resistance of pathogens causing microbial keratitis (a corneal inflammation) in the Clinical Department of Ophthalmology, Medical University of Silesia, Katowice. Despite intensive treatment, severe inflammation causes irreversible blindness in ∼7% of cases and eye loss (evisceration or enucleation of the eyeball) in ∼1% of cases at our hospital. The choice of a targeted drug depends on the culture result and drug resistance of the microorganism. This was a retrospective observation study. Conjunctival swabs and corneal scrapes were collected between January 1, 2013, and December 31, 2019, in the tertiary reference center for keratitis. The collected data included the type of material received, culture result, and antimicrobial susceptibilities. Of the 2482 samples analyzed, 679 were positive and 1803 were negative. Of the total pathogens isolated, 69.9% were Gram-positive bacteria, 20.8% were Gram-negative bacteria, and 7.1% were fungi. A significant increase in the number of Gram-positive methicillin-resistant Staphylococcus aureus and a partial increase in the number of Gram-negative beta-lactams-resistant bacteria were observed. All fungal species were sensitive to amphotericin B, 82.81% were sensitive to voriconazole, and 56.25% were sensitive to fluconazole. Dual drug therapy (levofloxacin and tobramycin) was the first-line treatment. Drug susceptibility testing of the cultured microorganisms is necessary to initiate targeted treatment. Increased drug resistance was observed in this study. In the present study, most bacteria were sensitive to fluoroquinolones. Ciprofloxacin therapy remains the recommended empirical treatment in microbial keratitis. According to our study, voriconazole remains a first-line antifungal drug, when a fungal infection is suspected.http://dx.doi.org/10.1155/2020/8851570 |
| spellingShingle | Klaudia Ulfik Sławomir Teper Michał Dembski Anna Nowińska Ewa Wróblewska-Czajka Edward Wylęgała Seven-Year Analysis of Microbial Keratitis Tendency at an Ophthalmology Department in Poland: A Single-Center Study Journal of Ophthalmology |
| title | Seven-Year Analysis of Microbial Keratitis Tendency at an Ophthalmology Department in Poland: A Single-Center Study |
| title_full | Seven-Year Analysis of Microbial Keratitis Tendency at an Ophthalmology Department in Poland: A Single-Center Study |
| title_fullStr | Seven-Year Analysis of Microbial Keratitis Tendency at an Ophthalmology Department in Poland: A Single-Center Study |
| title_full_unstemmed | Seven-Year Analysis of Microbial Keratitis Tendency at an Ophthalmology Department in Poland: A Single-Center Study |
| title_short | Seven-Year Analysis of Microbial Keratitis Tendency at an Ophthalmology Department in Poland: A Single-Center Study |
| title_sort | seven year analysis of microbial keratitis tendency at an ophthalmology department in poland a single center study |
| url | http://dx.doi.org/10.1155/2020/8851570 |
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