Cloning and expression of ricin a- and b-subunits genes in fusion constructions with dihydrofolate reductase gene in escheriсhia coli.
Recombinant chimeric constructions RTA-DHFR and RTВ-DHFR consisting of ricin Aand B-subunits genes (RTA and RTB) and dihydrofolate reductase (DHFR) gene were cloned and expressed in Escheriсhia coli. Antigenic properties of RTA and RTB retained in chimeric proteins RTA-DHFR and RTВ-DHFR.
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| Main Authors: | M. А. Gracheva, Z. М. Galushkina, N. N. Poletaeva, L. V. Verhovskaya, V. G. Lunin, V. I. Shvets |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
MIREA - Russian Technological University
2007-12-01
|
| Series: | Тонкие химические технологии |
| Online Access: | https://www.finechem-mirea.ru/jour/article/view/1353 |
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