Effects of fermented Crescentia cujete L. on the profile of haematology, clinical chemistry and circulatory CD4+/CD8+ in Sprague Dawley rats
Background: The calabash (Crescentia cujete L.) is a tropical fruit that offers numerous health benefits. Its fermented form (FC) has been found to affect the neurological system positively. However, its impact on haematology, clinical chemistry, and CD4+ and CD8+ levels has yet to be documented....
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| Format: | Article |
| Language: | English |
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Tripoli University
2024-09-01
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| Series: | Open Veterinary Journal |
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| Online Access: | https://www.openveterinaryjournal.com/?mno=207513 |
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| author | Agustina Dwi Wijayanti Yos Adi Prakoso Krestel Joy Viernes Isla |
| author_facet | Agustina Dwi Wijayanti Yos Adi Prakoso Krestel Joy Viernes Isla |
| author_sort | Agustina Dwi Wijayanti |
| collection | DOAJ |
| description | Background:
The calabash (Crescentia cujete L.) is a tropical fruit that offers numerous health benefits. Its fermented form (FC) has been found to affect the neurological system positively. However, its impact on haematology, clinical chemistry, and CD4+ and CD8+ levels has yet to be documented.
Aim:
Therefore, this study aims to examine the effects of FC on haematology, clinical chemistry, and the levels of CD4+ and CD8+ in the circulatory system using rat models.
Methods:
This study used twenty male Sprague Dawley rats. The rats were divided into group 1 (0 mg/kg BW FC), group 2 (50 mg/kg BW FC), group 3 (500 mg/kg BW FC), and group 4 (2,000 mg/kg BW FC). The treatment was administered using a gastric probe once daily for 14 days. On day 15, the blood samples were collected and tested against haematology, clinical chemistry, quality of the erythrocytes, and CD4+/CD8+. The data was then analysed using SPSS with p-value at 0.05.
Results:
The conducted study demonstrated that the utilisation of FC at varying doses did not have a significant impact on the haematological profile changes (p≥0.05), except for total lymphocytes and a decrease in the neutrophils/lymphocytes (N/L) ratio (p≤0.05). Furthermore, FC did not influence the changes in clinical chemistry, circulatory protein, and electrolyte levels in rat models compared to the control (p≥0.05). The utilisation of FC decreased the percentage of haemolysis and elevated the ATP concentration (p≤0.05). Additionally, the use of FC led to a significant increase in CD4+ and the ratio of CD4+/CD8+ (p≤0.05), while no significant effect was observed regarding CD8+ (p≥0.05).
Conclusion:
The study highlighted FC's beneficial effects on the haemorheology and immune system, specifically on the decrease in the percentage of haemolysis, elevated ATP concentration, number of lymphocytes, ratio N/L, CD4+, and the CD4+/CD8+ ratio, without causing significant changes to the haematological and clinical chemistry profiles in rat models. [Open Vet J 2024; 14(9.000): 2475-2483] |
| format | Article |
| id | doaj-art-8aa93ea7d9e54b5eb759d8b82aec018f |
| institution | OA Journals |
| issn | 2226-4485 2218-6050 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj-art-8aa93ea7d9e54b5eb759d8b82aec018f2025-08-20T01:47:36ZengTripoli UniversityOpen Veterinary Journal2226-44852218-60502024-09-011492475248310.5455/OVJ.2024.v14.i9.36207513Effects of fermented Crescentia cujete L. on the profile of haematology, clinical chemistry and circulatory CD4+/CD8+ in Sprague Dawley ratsAgustina Dwi Wijayanti0Yos Adi Prakoso1Krestel Joy Viernes Isla2Dept. Pharmacology, Faculty of Veterinary Medicine, University of Gadjah Mada, Yogyakarta, Indonesia, 55281 Dept. Pharmacology, Faculty of Veterinary Medicine, University of Wijaya Kusuma Surabaya, Indonesia, 60225 College of Veterinary Medicine, Tarlac Agricultural University, Philippines, 2306Background: The calabash (Crescentia cujete L.) is a tropical fruit that offers numerous health benefits. Its fermented form (FC) has been found to affect the neurological system positively. However, its impact on haematology, clinical chemistry, and CD4+ and CD8+ levels has yet to be documented. Aim: Therefore, this study aims to examine the effects of FC on haematology, clinical chemistry, and the levels of CD4+ and CD8+ in the circulatory system using rat models. Methods: This study used twenty male Sprague Dawley rats. The rats were divided into group 1 (0 mg/kg BW FC), group 2 (50 mg/kg BW FC), group 3 (500 mg/kg BW FC), and group 4 (2,000 mg/kg BW FC). The treatment was administered using a gastric probe once daily for 14 days. On day 15, the blood samples were collected and tested against haematology, clinical chemistry, quality of the erythrocytes, and CD4+/CD8+. The data was then analysed using SPSS with p-value at 0.05. Results: The conducted study demonstrated that the utilisation of FC at varying doses did not have a significant impact on the haematological profile changes (p≥0.05), except for total lymphocytes and a decrease in the neutrophils/lymphocytes (N/L) ratio (p≤0.05). Furthermore, FC did not influence the changes in clinical chemistry, circulatory protein, and electrolyte levels in rat models compared to the control (p≥0.05). The utilisation of FC decreased the percentage of haemolysis and elevated the ATP concentration (p≤0.05). Additionally, the use of FC led to a significant increase in CD4+ and the ratio of CD4+/CD8+ (p≤0.05), while no significant effect was observed regarding CD8+ (p≥0.05). Conclusion: The study highlighted FC's beneficial effects on the haemorheology and immune system, specifically on the decrease in the percentage of haemolysis, elevated ATP concentration, number of lymphocytes, ratio N/L, CD4+, and the CD4+/CD8+ ratio, without causing significant changes to the haematological and clinical chemistry profiles in rat models. [Open Vet J 2024; 14(9.000): 2475-2483]https://www.openveterinaryjournal.com/?mno=207513cd4+cd8+clinical chemistryfermented crescentia cujete (l)haematology |
| spellingShingle | Agustina Dwi Wijayanti Yos Adi Prakoso Krestel Joy Viernes Isla Effects of fermented Crescentia cujete L. on the profile of haematology, clinical chemistry and circulatory CD4+/CD8+ in Sprague Dawley rats Open Veterinary Journal cd4+ cd8+ clinical chemistry fermented crescentia cujete (l) haematology |
| title | Effects of fermented Crescentia cujete L. on the profile of haematology, clinical chemistry and circulatory CD4+/CD8+ in Sprague Dawley rats |
| title_full | Effects of fermented Crescentia cujete L. on the profile of haematology, clinical chemistry and circulatory CD4+/CD8+ in Sprague Dawley rats |
| title_fullStr | Effects of fermented Crescentia cujete L. on the profile of haematology, clinical chemistry and circulatory CD4+/CD8+ in Sprague Dawley rats |
| title_full_unstemmed | Effects of fermented Crescentia cujete L. on the profile of haematology, clinical chemistry and circulatory CD4+/CD8+ in Sprague Dawley rats |
| title_short | Effects of fermented Crescentia cujete L. on the profile of haematology, clinical chemistry and circulatory CD4+/CD8+ in Sprague Dawley rats |
| title_sort | effects of fermented crescentia cujete l on the profile of haematology clinical chemistry and circulatory cd4 cd8 in sprague dawley rats |
| topic | cd4+ cd8+ clinical chemistry fermented crescentia cujete (l) haematology |
| url | https://www.openveterinaryjournal.com/?mno=207513 |
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