Postbiotic, anti-inflammatory, and immunomodulatory effects of aqueous microbial lysozyme in broiler chickens

Lysozymes, efficient alternative supplements to antibiotics, have several benefits in poultry production. In the present study, 120, one–day–old, Ross 308 broiler chickens of mixed sex, were allocated into 2 equal groups, lysozyme treated group (LTG) and lysozyme free group (LFG), to evaluate the ef...

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Main Authors: Mustafa Bastamy, Ismail Raheel, Ahmed Elbestawy, Mohamed Diab, Enas Hammad, Lamiaa Elebeedy, Amal M. El-Barbary, Ghadeer M. Albadrani, Mohamed M. Abdel-Daim, Mervat A. Abdel-Latif, Ahmed Orabi
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Animal Biotechnology
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Online Access:https://www.tandfonline.com/doi/10.1080/10495398.2024.2309955
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author Mustafa Bastamy
Ismail Raheel
Ahmed Elbestawy
Mohamed Diab
Enas Hammad
Lamiaa Elebeedy
Amal M. El-Barbary
Ghadeer M. Albadrani
Mohamed M. Abdel-Daim
Mervat A. Abdel-Latif
Ahmed Orabi
author_facet Mustafa Bastamy
Ismail Raheel
Ahmed Elbestawy
Mohamed Diab
Enas Hammad
Lamiaa Elebeedy
Amal M. El-Barbary
Ghadeer M. Albadrani
Mohamed M. Abdel-Daim
Mervat A. Abdel-Latif
Ahmed Orabi
author_sort Mustafa Bastamy
collection DOAJ
description Lysozymes, efficient alternative supplements to antibiotics, have several benefits in poultry production. In the present study, 120, one–day–old, Ross 308 broiler chickens of mixed sex, were allocated into 2 equal groups, lysozyme treated group (LTG) and lysozyme free group (LFG), to evaluate the efficacy of lysozyme (Lysonir®) usage via both drinking water (thrice) and spray (once). LTG had better (p = 0.042) FCR, and higher European production efficiency factor compared to LFG (p = 0.042). The intestinal integrity score of LTG was decreased (p = 0.242) compared to that of LFG; 0.2 vs. 0.7. Higher (p ≤ 0.001) intestinal Lactobacillus counts were detected in chickens of LTG. Decreased (p ≤ 0.001) IL-1β and CXCL8 values were reported in LTG. The cellular immune modulation showed higher (p ≤ 0.001) opsonic activity (MΦ and phagocytic index) in LTG vs. LFG at 25 and 35 days. Also, higher (p ≤ 0.001) local, IgA, and humoral, HI titers, for both Newcastle, and avian influenza H5 viruses were found in LTG compared to LFG. In conclusion, microbial lysozyme could improve feed efficiency, intestinal integrity, Lactobacillus counts, anti-inflammatory, and immune responses in broiler chickens.
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spelling doaj-art-8aa7cbb3a89b455aad1f2a6957c5dc852025-08-20T02:50:22ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782024-12-0135110.1080/10495398.2024.2309955Postbiotic, anti-inflammatory, and immunomodulatory effects of aqueous microbial lysozyme in broiler chickensMustafa Bastamy0Ismail Raheel1Ahmed Elbestawy2Mohamed Diab3Enas Hammad4Lamiaa Elebeedy5Amal M. El-Barbary6Ghadeer M. Albadrani7Mohamed M. Abdel-Daim8Mervat A. Abdel-Latif9Ahmed Orabi10Department of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, EgyptDepartment of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suief University, Beni-Suief, EgyptDepartment of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Damanhour University, Elbeheira, EgyptDepartment of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, New Valley University, El Kharga, EgyptAgricultural Research Center (ARC), Animal Health Research Institute-Mansoura Provincial Lab (AHRI-Mansoura), Dokki, Giza, EgyptFaculty of Pharmacy, New Valley University, El Kharga, EgyptPoultry Breeding Research Department, Animal Production Research Institute, Agriculture Research Center, Giza, EgyptDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi ArabiaDepartment of Pharmaceutical Sciences, Pharmacy Program, Batterjee Medical College, Jeddah, Saudi ArabiaDepartment of Nutrition and Veterinary Clinical Nutrition, Faculty of Veterinary Medicine, Damanhour University, Damanhour, EgyptDepartment of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza, EgyptLysozymes, efficient alternative supplements to antibiotics, have several benefits in poultry production. In the present study, 120, one–day–old, Ross 308 broiler chickens of mixed sex, were allocated into 2 equal groups, lysozyme treated group (LTG) and lysozyme free group (LFG), to evaluate the efficacy of lysozyme (Lysonir®) usage via both drinking water (thrice) and spray (once). LTG had better (p = 0.042) FCR, and higher European production efficiency factor compared to LFG (p = 0.042). The intestinal integrity score of LTG was decreased (p = 0.242) compared to that of LFG; 0.2 vs. 0.7. Higher (p ≤ 0.001) intestinal Lactobacillus counts were detected in chickens of LTG. Decreased (p ≤ 0.001) IL-1β and CXCL8 values were reported in LTG. The cellular immune modulation showed higher (p ≤ 0.001) opsonic activity (MΦ and phagocytic index) in LTG vs. LFG at 25 and 35 days. Also, higher (p ≤ 0.001) local, IgA, and humoral, HI titers, for both Newcastle, and avian influenza H5 viruses were found in LTG compared to LFG. In conclusion, microbial lysozyme could improve feed efficiency, intestinal integrity, Lactobacillus counts, anti-inflammatory, and immune responses in broiler chickens.https://www.tandfonline.com/doi/10.1080/10495398.2024.2309955Lysozymepostbioticanti-inflammatoryimmunomodulationbroiler chickens
spellingShingle Mustafa Bastamy
Ismail Raheel
Ahmed Elbestawy
Mohamed Diab
Enas Hammad
Lamiaa Elebeedy
Amal M. El-Barbary
Ghadeer M. Albadrani
Mohamed M. Abdel-Daim
Mervat A. Abdel-Latif
Ahmed Orabi
Postbiotic, anti-inflammatory, and immunomodulatory effects of aqueous microbial lysozyme in broiler chickens
Animal Biotechnology
Lysozyme
postbiotic
anti-inflammatory
immunomodulation
broiler chickens
title Postbiotic, anti-inflammatory, and immunomodulatory effects of aqueous microbial lysozyme in broiler chickens
title_full Postbiotic, anti-inflammatory, and immunomodulatory effects of aqueous microbial lysozyme in broiler chickens
title_fullStr Postbiotic, anti-inflammatory, and immunomodulatory effects of aqueous microbial lysozyme in broiler chickens
title_full_unstemmed Postbiotic, anti-inflammatory, and immunomodulatory effects of aqueous microbial lysozyme in broiler chickens
title_short Postbiotic, anti-inflammatory, and immunomodulatory effects of aqueous microbial lysozyme in broiler chickens
title_sort postbiotic anti inflammatory and immunomodulatory effects of aqueous microbial lysozyme in broiler chickens
topic Lysozyme
postbiotic
anti-inflammatory
immunomodulation
broiler chickens
url https://www.tandfonline.com/doi/10.1080/10495398.2024.2309955
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