Joint subarray acoustic tweezers enable controllable cell translation, rotation, and deformation

Abstract Contactless microscale tweezers are highly effective tools for manipulating, patterning, and assembling bioparticles. However, current tweezers are limited in their ability to comprehensively manipulate bioparticles, providing only partial control over the six fundamental motions (three tra...

Full description

Saved in:
Bibliographic Details
Main Authors: Liang Shen, Zhenhua Tian, Kaichun Yang, Joseph Rich, Jianping Xia, Neil Upreti, Jinxin Zhang, Chuyi Chen, Nanjing Hao, Zhichao Pei, Tony Jun Huang
Format: Article
Language:English
Published: Nature Portfolio 2024-10-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-024-52686-8
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850181504933560320
author Liang Shen
Zhenhua Tian
Kaichun Yang
Joseph Rich
Jianping Xia
Neil Upreti
Jinxin Zhang
Chuyi Chen
Nanjing Hao
Zhichao Pei
Tony Jun Huang
author_facet Liang Shen
Zhenhua Tian
Kaichun Yang
Joseph Rich
Jianping Xia
Neil Upreti
Jinxin Zhang
Chuyi Chen
Nanjing Hao
Zhichao Pei
Tony Jun Huang
author_sort Liang Shen
collection DOAJ
description Abstract Contactless microscale tweezers are highly effective tools for manipulating, patterning, and assembling bioparticles. However, current tweezers are limited in their ability to comprehensively manipulate bioparticles, providing only partial control over the six fundamental motions (three translational and three rotational motions). This study presents a joint subarray acoustic tweezers platform that leverages acoustic radiation force and viscous torque to control the six fundamental motions of single bioparticles. This breakthrough is significant as our manipulation mechanism allows for controlling the three translational and three rotational motions of single cells, as well as enabling complex manipulation that combines controlled translational and rotational motions. Moreover, our tweezers can gradually increase the load on an acoustically trapped cell to achieve controllable cell deformation critical for characterizing cell mechanical properties. Furthermore, our platform allows for three-dimensional (3D) imaging of bioparticles without using complex confocal microscopy by rotating bioparticles with acoustic tweezers and taking images of each orientation using a standard microscope. With these capabilities, we anticipate the JSAT platform to play a pivotal role in various applications, including 3D imaging, tissue engineering, disease diagnostics, and drug testing.
format Article
id doaj-art-8aa1b21fde1a46119e604ea5498fdb12
institution OA Journals
issn 2041-1723
language English
publishDate 2024-10-01
publisher Nature Portfolio
record_format Article
series Nature Communications
spelling doaj-art-8aa1b21fde1a46119e604ea5498fdb122025-08-20T02:17:53ZengNature PortfolioNature Communications2041-17232024-10-0115111110.1038/s41467-024-52686-8Joint subarray acoustic tweezers enable controllable cell translation, rotation, and deformationLiang Shen0Zhenhua Tian1Kaichun Yang2Joseph Rich3Jianping Xia4Neil Upreti5Jinxin Zhang6Chuyi Chen7Nanjing Hao8Zhichao Pei9Tony Jun Huang10Department of Mechanical Engineering and Materials Science, Duke UniversityDepartment of Mechanical Engineering, Virginia Polytechnical Institute and State UniversityDepartment of Mechanical Engineering and Materials Science, Duke UniversityDepartment of Biomedical Engineering, Duke UniversityDepartment of Mechanical Engineering and Materials Science, Duke UniversityDepartment of Mechanical Engineering and Materials Science, Duke UniversityDepartment of Mechanical Engineering and Materials Science, Duke UniversityDepartment of Mechanical Engineering and Materials Science, Duke UniversityDepartment of Mechanical Engineering and Materials Science, Duke UniversityDepartment of Mechanical Engineering and Materials Science, Duke UniversityDepartment of Mechanical Engineering and Materials Science, Duke UniversityAbstract Contactless microscale tweezers are highly effective tools for manipulating, patterning, and assembling bioparticles. However, current tweezers are limited in their ability to comprehensively manipulate bioparticles, providing only partial control over the six fundamental motions (three translational and three rotational motions). This study presents a joint subarray acoustic tweezers platform that leverages acoustic radiation force and viscous torque to control the six fundamental motions of single bioparticles. This breakthrough is significant as our manipulation mechanism allows for controlling the three translational and three rotational motions of single cells, as well as enabling complex manipulation that combines controlled translational and rotational motions. Moreover, our tweezers can gradually increase the load on an acoustically trapped cell to achieve controllable cell deformation critical for characterizing cell mechanical properties. Furthermore, our platform allows for three-dimensional (3D) imaging of bioparticles without using complex confocal microscopy by rotating bioparticles with acoustic tweezers and taking images of each orientation using a standard microscope. With these capabilities, we anticipate the JSAT platform to play a pivotal role in various applications, including 3D imaging, tissue engineering, disease diagnostics, and drug testing.https://doi.org/10.1038/s41467-024-52686-8
spellingShingle Liang Shen
Zhenhua Tian
Kaichun Yang
Joseph Rich
Jianping Xia
Neil Upreti
Jinxin Zhang
Chuyi Chen
Nanjing Hao
Zhichao Pei
Tony Jun Huang
Joint subarray acoustic tweezers enable controllable cell translation, rotation, and deformation
Nature Communications
title Joint subarray acoustic tweezers enable controllable cell translation, rotation, and deformation
title_full Joint subarray acoustic tweezers enable controllable cell translation, rotation, and deformation
title_fullStr Joint subarray acoustic tweezers enable controllable cell translation, rotation, and deformation
title_full_unstemmed Joint subarray acoustic tweezers enable controllable cell translation, rotation, and deformation
title_short Joint subarray acoustic tweezers enable controllable cell translation, rotation, and deformation
title_sort joint subarray acoustic tweezers enable controllable cell translation rotation and deformation
url https://doi.org/10.1038/s41467-024-52686-8
work_keys_str_mv AT liangshen jointsubarrayacoustictweezersenablecontrollablecelltranslationrotationanddeformation
AT zhenhuatian jointsubarrayacoustictweezersenablecontrollablecelltranslationrotationanddeformation
AT kaichunyang jointsubarrayacoustictweezersenablecontrollablecelltranslationrotationanddeformation
AT josephrich jointsubarrayacoustictweezersenablecontrollablecelltranslationrotationanddeformation
AT jianpingxia jointsubarrayacoustictweezersenablecontrollablecelltranslationrotationanddeformation
AT neilupreti jointsubarrayacoustictweezersenablecontrollablecelltranslationrotationanddeformation
AT jinxinzhang jointsubarrayacoustictweezersenablecontrollablecelltranslationrotationanddeformation
AT chuyichen jointsubarrayacoustictweezersenablecontrollablecelltranslationrotationanddeformation
AT nanjinghao jointsubarrayacoustictweezersenablecontrollablecelltranslationrotationanddeformation
AT zhichaopei jointsubarrayacoustictweezersenablecontrollablecelltranslationrotationanddeformation
AT tonyjunhuang jointsubarrayacoustictweezersenablecontrollablecelltranslationrotationanddeformation