Protective Effect of Zeaxanthin from <i>Lycium barbarum</i> L. on Ultraviolet B-Induced Skin Photodamage in Mice Through Nrf2-Related Pathway

Ultraviolet (UV) radiation is a predominant cause of skin damage, with UVB leading to more severe harm compared to UVA. <i>Lycium barbarum</i> L. (<i>L. barbarum</i>) is known for its high carotenoid content and has shown great potential in mitigating UVB-induced skin damage....

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Main Authors: Lin Zhu, Qiruonan Shen, Yujuan Xu, Chunmei Li
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Antioxidants
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Online Access:https://www.mdpi.com/2076-3921/14/6/632
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author Lin Zhu
Qiruonan Shen
Yujuan Xu
Chunmei Li
author_facet Lin Zhu
Qiruonan Shen
Yujuan Xu
Chunmei Li
author_sort Lin Zhu
collection DOAJ
description Ultraviolet (UV) radiation is a predominant cause of skin damage, with UVB leading to more severe harm compared to UVA. <i>Lycium barbarum</i> L. (<i>L. barbarum</i>) is known for its high carotenoid content and has shown great potential in mitigating UVB-induced skin damage. This study investigated the protective effect and mechanism of zeaxanthin from <i>L. barbarum</i> on UVB-damaged skin in BALB/c mice. The results demonstrated that zeaxanthin effectively alleviated the UVB-injured appearance of mouse skin. Histological analyses revealed a reduction in epidermal thickness by 30% and 61% with low and high doses of zeaxanthin, respectively, compared to the model group. Zeaxanthin also inhibited the degeneration of elastic and collagen fibers. Further investigations indicated that the protective mechanism of zeaxanthin was not involved with inflammation suppression. Instead, it activated nuclear factor erythroid 2-related factor 2 (Nrf2) to approximately 3 times the level of the model group, significantly promoting the expression of various antioxidant enzymes and enhancing the total antioxidant capacity of skin tissue, subsequently reducing oxidative stress. Zeaxanthin also downregulated the expression of matrix metalloproteinases, reducing collagen degradation by 35% compared to the model group, which led to improved skin tissue structure and protection against UVB-induced photodamage. These findings provided a theoretical basis for the advanced development and high-value utilization of carotenoids in <i>L. barbarum</i>.
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spelling doaj-art-8a9fb114043a408c8a5ac74fff2e5df92025-08-20T02:24:22ZengMDPI AGAntioxidants2076-39212025-05-0114663210.3390/antiox14060632Protective Effect of Zeaxanthin from <i>Lycium barbarum</i> L. on Ultraviolet B-Induced Skin Photodamage in Mice Through Nrf2-Related PathwayLin Zhu0Qiruonan Shen1Yujuan Xu2Chunmei Li3College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaSericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Products, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaUltraviolet (UV) radiation is a predominant cause of skin damage, with UVB leading to more severe harm compared to UVA. <i>Lycium barbarum</i> L. (<i>L. barbarum</i>) is known for its high carotenoid content and has shown great potential in mitigating UVB-induced skin damage. This study investigated the protective effect and mechanism of zeaxanthin from <i>L. barbarum</i> on UVB-damaged skin in BALB/c mice. The results demonstrated that zeaxanthin effectively alleviated the UVB-injured appearance of mouse skin. Histological analyses revealed a reduction in epidermal thickness by 30% and 61% with low and high doses of zeaxanthin, respectively, compared to the model group. Zeaxanthin also inhibited the degeneration of elastic and collagen fibers. Further investigations indicated that the protective mechanism of zeaxanthin was not involved with inflammation suppression. Instead, it activated nuclear factor erythroid 2-related factor 2 (Nrf2) to approximately 3 times the level of the model group, significantly promoting the expression of various antioxidant enzymes and enhancing the total antioxidant capacity of skin tissue, subsequently reducing oxidative stress. Zeaxanthin also downregulated the expression of matrix metalloproteinases, reducing collagen degradation by 35% compared to the model group, which led to improved skin tissue structure and protection against UVB-induced photodamage. These findings provided a theoretical basis for the advanced development and high-value utilization of carotenoids in <i>L. barbarum</i>.https://www.mdpi.com/2076-3921/14/6/632UVBskin<i>Lycium barbarum</i> L.zeaxanthinappearancematrix metalloproteinases
spellingShingle Lin Zhu
Qiruonan Shen
Yujuan Xu
Chunmei Li
Protective Effect of Zeaxanthin from <i>Lycium barbarum</i> L. on Ultraviolet B-Induced Skin Photodamage in Mice Through Nrf2-Related Pathway
Antioxidants
UVB
skin
<i>Lycium barbarum</i> L.
zeaxanthin
appearance
matrix metalloproteinases
title Protective Effect of Zeaxanthin from <i>Lycium barbarum</i> L. on Ultraviolet B-Induced Skin Photodamage in Mice Through Nrf2-Related Pathway
title_full Protective Effect of Zeaxanthin from <i>Lycium barbarum</i> L. on Ultraviolet B-Induced Skin Photodamage in Mice Through Nrf2-Related Pathway
title_fullStr Protective Effect of Zeaxanthin from <i>Lycium barbarum</i> L. on Ultraviolet B-Induced Skin Photodamage in Mice Through Nrf2-Related Pathway
title_full_unstemmed Protective Effect of Zeaxanthin from <i>Lycium barbarum</i> L. on Ultraviolet B-Induced Skin Photodamage in Mice Through Nrf2-Related Pathway
title_short Protective Effect of Zeaxanthin from <i>Lycium barbarum</i> L. on Ultraviolet B-Induced Skin Photodamage in Mice Through Nrf2-Related Pathway
title_sort protective effect of zeaxanthin from i lycium barbarum i l on ultraviolet b induced skin photodamage in mice through nrf2 related pathway
topic UVB
skin
<i>Lycium barbarum</i> L.
zeaxanthin
appearance
matrix metalloproteinases
url https://www.mdpi.com/2076-3921/14/6/632
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AT qiruonanshen protectiveeffectofzeaxanthinfromilyciumbarbarumilonultravioletbinducedskinphotodamageinmicethroughnrf2relatedpathway
AT yujuanxu protectiveeffectofzeaxanthinfromilyciumbarbarumilonultravioletbinducedskinphotodamageinmicethroughnrf2relatedpathway
AT chunmeili protectiveeffectofzeaxanthinfromilyciumbarbarumilonultravioletbinducedskinphotodamageinmicethroughnrf2relatedpathway