Minimum Effective Dose of Cattle and Sheep BSE for Oral Sheep Infection.
The minimum dose required to cause infection of Romney and Suffolk sheep of the ARQ/ARQ or ARQ/ARR prion protein gene genotypes following oral inoculation with Romney or Suffolk a sheep Bovine spongiform encephalopathy (BSE)-derived or cattle BSE-derived agent was investigated using doses ranging fr...
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0151440&type=printable |
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| author | Gillian McGovern Stuart Martin Martin Jeffrey Glenda Dexter Steve A C Hawkins Sue J Bellworthy Lisa Thurston Lynne Algar Lorenzo González |
| author_facet | Gillian McGovern Stuart Martin Martin Jeffrey Glenda Dexter Steve A C Hawkins Sue J Bellworthy Lisa Thurston Lynne Algar Lorenzo González |
| author_sort | Gillian McGovern |
| collection | DOAJ |
| description | The minimum dose required to cause infection of Romney and Suffolk sheep of the ARQ/ARQ or ARQ/ARR prion protein gene genotypes following oral inoculation with Romney or Suffolk a sheep Bovine spongiform encephalopathy (BSE)-derived or cattle BSE-derived agent was investigated using doses ranging from 0.0005g to 5g. ARQ/ARQ sheep which were methionine (M) / threonine (T) heterozygous or T/T homozygous at codon 112 of the Prnp gene, dosed ARQ/ARR sheep and undosed controls did not show any evidence of infection. Within groups of susceptible sheep, the minimum effective oral dose of BSE was found to be 0.05g, with higher attack rates following inoculation with the 5g dose. Surprisingly, this study found no effect of dose on survival time suggesting a possible lack of homogeneity within the inoculum. All clinical BSE cases showed PrPd accumulation in brain; however, following cattle BSE inoculation, LRS involvement within Romney recipients was found to be significantly lower than within the Suffolk sheep inoculated group which is in agreement with previous reports. |
| format | Article |
| id | doaj-art-8a8c7ba9aa0749e0aa3aba4cb4bca8cc |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-8a8c7ba9aa0749e0aa3aba4cb4bca8cc2025-08-20T03:10:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01113e015144010.1371/journal.pone.0151440Minimum Effective Dose of Cattle and Sheep BSE for Oral Sheep Infection.Gillian McGovernStuart MartinMartin JeffreyGlenda DexterSteve A C HawkinsSue J BellworthyLisa ThurstonLynne AlgarLorenzo GonzálezThe minimum dose required to cause infection of Romney and Suffolk sheep of the ARQ/ARQ or ARQ/ARR prion protein gene genotypes following oral inoculation with Romney or Suffolk a sheep Bovine spongiform encephalopathy (BSE)-derived or cattle BSE-derived agent was investigated using doses ranging from 0.0005g to 5g. ARQ/ARQ sheep which were methionine (M) / threonine (T) heterozygous or T/T homozygous at codon 112 of the Prnp gene, dosed ARQ/ARR sheep and undosed controls did not show any evidence of infection. Within groups of susceptible sheep, the minimum effective oral dose of BSE was found to be 0.05g, with higher attack rates following inoculation with the 5g dose. Surprisingly, this study found no effect of dose on survival time suggesting a possible lack of homogeneity within the inoculum. All clinical BSE cases showed PrPd accumulation in brain; however, following cattle BSE inoculation, LRS involvement within Romney recipients was found to be significantly lower than within the Suffolk sheep inoculated group which is in agreement with previous reports.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0151440&type=printable |
| spellingShingle | Gillian McGovern Stuart Martin Martin Jeffrey Glenda Dexter Steve A C Hawkins Sue J Bellworthy Lisa Thurston Lynne Algar Lorenzo González Minimum Effective Dose of Cattle and Sheep BSE for Oral Sheep Infection. PLoS ONE |
| title | Minimum Effective Dose of Cattle and Sheep BSE for Oral Sheep Infection. |
| title_full | Minimum Effective Dose of Cattle and Sheep BSE for Oral Sheep Infection. |
| title_fullStr | Minimum Effective Dose of Cattle and Sheep BSE for Oral Sheep Infection. |
| title_full_unstemmed | Minimum Effective Dose of Cattle and Sheep BSE for Oral Sheep Infection. |
| title_short | Minimum Effective Dose of Cattle and Sheep BSE for Oral Sheep Infection. |
| title_sort | minimum effective dose of cattle and sheep bse for oral sheep infection |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0151440&type=printable |
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