A Novel Ephemero- and a New CHeRI Orbivirus Isolated from a Dead Farmed White-Tailed Deer (<i>Odocoileus virginianus</i>) in Florida, USA
A novel ephemeral fever rhabdovirus and a CHeRI orbivirus of a previously unidentified genetic lineage were isolated in mosquito cell line C6/36 cells as co-infecting agents from the spleen tissue of a dead farmed white-tailed deer (WTD; <i>Odocoileus virginianus</i>) in Florida. We desi...
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2025-04-01
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| Online Access: | https://www.mdpi.com/1999-4915/17/5/614 |
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| author | Emily DeRuyter Pedro H. O. Viadanna Kristen Wilson Zoe White Amira Richardson Merrie Urban Pacharapong Khrongsee Thais C. S. Rodrigues Thomas B. Waltzek Juan M. Campos Krauer Samantha M. Wisely Kuttichantran Subramaniam John A. Lednicky |
| author_facet | Emily DeRuyter Pedro H. O. Viadanna Kristen Wilson Zoe White Amira Richardson Merrie Urban Pacharapong Khrongsee Thais C. S. Rodrigues Thomas B. Waltzek Juan M. Campos Krauer Samantha M. Wisely Kuttichantran Subramaniam John A. Lednicky |
| author_sort | Emily DeRuyter |
| collection | DOAJ |
| description | A novel ephemeral fever rhabdovirus and a CHeRI orbivirus of a previously unidentified genetic lineage were isolated in mosquito cell line C6/36 cells as co-infecting agents from the spleen tissue of a dead farmed white-tailed deer (WTD; <i>Odocoileus virginianus</i>) in Florida. We designated the ephemeral fever rhabdovirus as Hardee County ephemerovirus 1, strain CHeRI ephemerovirus 1. The genetic sequences of the CHeRI orbivirus isolated in this work differ significantly from those of three previously described CHeRI orbivirus lineages. We designated this new virus as CHeRI orbivirus 4, strain CHeRI orbivirus 4-1. Whereas it remains unknown whether one, both, or none of the viruses contributed to the pathology, gross observations revealed that the dead WTD had severely congested and hemorrhagic lungs, and that its heart, kidneys, and spleen were also congested. |
| format | Article |
| id | doaj-art-8a871c23a5ac4a1996a479f1e705dcc6 |
| institution | OA Journals |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-8a871c23a5ac4a1996a479f1e705dcc62025-08-20T01:56:42ZengMDPI AGViruses1999-49152025-04-0117561410.3390/v17050614A Novel Ephemero- and a New CHeRI Orbivirus Isolated from a Dead Farmed White-Tailed Deer (<i>Odocoileus virginianus</i>) in Florida, USAEmily DeRuyter0Pedro H. O. Viadanna1Kristen Wilson2Zoe White3Amira Richardson4Merrie Urban5Pacharapong Khrongsee6Thais C. S. Rodrigues7Thomas B. Waltzek8Juan M. Campos Krauer9Samantha M. Wisely10Kuttichantran Subramaniam11John A. Lednicky12Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, FL 32610, USAWashington Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Washington State University, Pullman, WA 99163, USAEmerging Pathogens Institute, University of Florida, 2055 Mowry Rd., Gainesville, FL 32610, USAEmerging Pathogens Institute, University of Florida, 2055 Mowry Rd., Gainesville, FL 32610, USADepartment of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USADepartment of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USAEmerging Pathogens Institute, University of Florida, 2055 Mowry Rd., Gainesville, FL 32610, USAOperation GRACE, National Marine Mammal Foundation, San Diego, CA 92106, USAWashington Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Washington State University, Pullman, WA 99163, USADepartment of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USAEmerging Pathogens Institute, University of Florida, 2055 Mowry Rd., Gainesville, FL 32610, USAEmerging Pathogens Institute, University of Florida, 2055 Mowry Rd., Gainesville, FL 32610, USADepartment of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, FL 32610, USAA novel ephemeral fever rhabdovirus and a CHeRI orbivirus of a previously unidentified genetic lineage were isolated in mosquito cell line C6/36 cells as co-infecting agents from the spleen tissue of a dead farmed white-tailed deer (WTD; <i>Odocoileus virginianus</i>) in Florida. We designated the ephemeral fever rhabdovirus as Hardee County ephemerovirus 1, strain CHeRI ephemerovirus 1. The genetic sequences of the CHeRI orbivirus isolated in this work differ significantly from those of three previously described CHeRI orbivirus lineages. We designated this new virus as CHeRI orbivirus 4, strain CHeRI orbivirus 4-1. Whereas it remains unknown whether one, both, or none of the viruses contributed to the pathology, gross observations revealed that the dead WTD had severely congested and hemorrhagic lungs, and that its heart, kidneys, and spleen were also congested.https://www.mdpi.com/1999-4915/17/5/614vectorephemeral feverwhite-tailed deerdeer farmingFlorida deer farmingorbiviruses |
| spellingShingle | Emily DeRuyter Pedro H. O. Viadanna Kristen Wilson Zoe White Amira Richardson Merrie Urban Pacharapong Khrongsee Thais C. S. Rodrigues Thomas B. Waltzek Juan M. Campos Krauer Samantha M. Wisely Kuttichantran Subramaniam John A. Lednicky A Novel Ephemero- and a New CHeRI Orbivirus Isolated from a Dead Farmed White-Tailed Deer (<i>Odocoileus virginianus</i>) in Florida, USA Viruses vector ephemeral fever white-tailed deer deer farming Florida deer farming orbiviruses |
| title | A Novel Ephemero- and a New CHeRI Orbivirus Isolated from a Dead Farmed White-Tailed Deer (<i>Odocoileus virginianus</i>) in Florida, USA |
| title_full | A Novel Ephemero- and a New CHeRI Orbivirus Isolated from a Dead Farmed White-Tailed Deer (<i>Odocoileus virginianus</i>) in Florida, USA |
| title_fullStr | A Novel Ephemero- and a New CHeRI Orbivirus Isolated from a Dead Farmed White-Tailed Deer (<i>Odocoileus virginianus</i>) in Florida, USA |
| title_full_unstemmed | A Novel Ephemero- and a New CHeRI Orbivirus Isolated from a Dead Farmed White-Tailed Deer (<i>Odocoileus virginianus</i>) in Florida, USA |
| title_short | A Novel Ephemero- and a New CHeRI Orbivirus Isolated from a Dead Farmed White-Tailed Deer (<i>Odocoileus virginianus</i>) in Florida, USA |
| title_sort | novel ephemero and a new cheri orbivirus isolated from a dead farmed white tailed deer i odocoileus virginianus i in florida usa |
| topic | vector ephemeral fever white-tailed deer deer farming Florida deer farming orbiviruses |
| url | https://www.mdpi.com/1999-4915/17/5/614 |
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