Effect of IBA and BAP on Shoot Growth of Tawangmangu Tangerine (Citrus reticulate) by In-Vitro

Tawangmangu tangerine (Citrus reticulate Blanco subsp. tawangmangu) is one of prime local fruit from Tawangmangu region, Karanganyar, Central Java. This tangerine have good appearance, easy to peeled, sweet flavored fruit, and high production rate. However at 1984 Tawangmangu tangerine run into depr...

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Main Authors: Arif Ardianto Sofian, Erma Prihastanti, Sri Widodo Agung Suedy
Format: Article
Language:English
Published: Universitas Negeri Semarang 2018-08-01
Series:Biosaintifika: Journal of Biology & Biology Education
Subjects:
Online Access:https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/14977
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author Arif Ardianto Sofian
Erma Prihastanti
Sri Widodo Agung Suedy
author_facet Arif Ardianto Sofian
Erma Prihastanti
Sri Widodo Agung Suedy
author_sort Arif Ardianto Sofian
collection DOAJ
description Tawangmangu tangerine (Citrus reticulate Blanco subsp. tawangmangu) is one of prime local fruit from Tawangmangu region, Karanganyar, Central Java. This tangerine have good appearance, easy to peeled, sweet flavored fruit, and high production rate. However at 1984 Tawangmangu tangerine run into depreciation of population caused by CVPD (Citrus Vein Phloem Degeneration). As a result, this variety listed as endangeredThe effort of consevation by culture tissue technique can order to obtain prime seeds that free from virus or disease. This study was aimed to understand the effect of IBA and BAP in culture medium on growth of apical and lateral shoot of Tawangmangu tangerine. The treatment provided were IBA (0 ppm, 0.5 ppm, and 1 ppm) and BAP (0 ppm, 1 ppm and 2 ppm). The treatment in this research was using complete randomized factorial design in 9 different treatment. This research resulted that added combination of IBA and BAP affecting growth of Tawangmangu tangerine but the interaction of IBA and BAP did not happen. The highest shoot length occurs on treatment IBA 1 ppm+BAP 2 ppm while when the fastest shoot appears occurs on treatment IBA 0 ppm+ BAP 2 ppm and the highest shoot formed on treatment IBA 0.5 ppm+BAP 2 ppm. Benefit of the research Efforts to conserve Tawangmangu orange plants need to be done to maintain the diversity of germplasm and support the cultivation in a manner. One technique that can support the conservation of plants is by tissue culture techniques.
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spelling doaj-art-8a5bab270d7c420b96b183cb199cf0782025-08-20T02:09:18ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102018-08-0110237938710.15294/biosaintifika.v10i2.149777533Effect of IBA and BAP on Shoot Growth of Tawangmangu Tangerine (Citrus reticulate) by In-VitroArif Ardianto Sofian0Erma Prihastanti1Sri Widodo Agung Suedy2Department of Biology, Faculty of Science and Mathematics, Universitas DiponegoroDepartment of Biology, Faculty of Science and Mathematics, Universitas DiponegoroDepartment of Biology, Faculty of Science and Mathematics, Universitas DiponegoroTawangmangu tangerine (Citrus reticulate Blanco subsp. tawangmangu) is one of prime local fruit from Tawangmangu region, Karanganyar, Central Java. This tangerine have good appearance, easy to peeled, sweet flavored fruit, and high production rate. However at 1984 Tawangmangu tangerine run into depreciation of population caused by CVPD (Citrus Vein Phloem Degeneration). As a result, this variety listed as endangeredThe effort of consevation by culture tissue technique can order to obtain prime seeds that free from virus or disease. This study was aimed to understand the effect of IBA and BAP in culture medium on growth of apical and lateral shoot of Tawangmangu tangerine. The treatment provided were IBA (0 ppm, 0.5 ppm, and 1 ppm) and BAP (0 ppm, 1 ppm and 2 ppm). The treatment in this research was using complete randomized factorial design in 9 different treatment. This research resulted that added combination of IBA and BAP affecting growth of Tawangmangu tangerine but the interaction of IBA and BAP did not happen. The highest shoot length occurs on treatment IBA 1 ppm+BAP 2 ppm while when the fastest shoot appears occurs on treatment IBA 0 ppm+ BAP 2 ppm and the highest shoot formed on treatment IBA 0.5 ppm+BAP 2 ppm. Benefit of the research Efforts to conserve Tawangmangu orange plants need to be done to maintain the diversity of germplasm and support the cultivation in a manner. One technique that can support the conservation of plants is by tissue culture techniques.https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/14977ibabapshoot growthtawangmangu tangerine
spellingShingle Arif Ardianto Sofian
Erma Prihastanti
Sri Widodo Agung Suedy
Effect of IBA and BAP on Shoot Growth of Tawangmangu Tangerine (Citrus reticulate) by In-Vitro
Biosaintifika: Journal of Biology & Biology Education
iba
bap
shoot growth
tawangmangu tangerine
title Effect of IBA and BAP on Shoot Growth of Tawangmangu Tangerine (Citrus reticulate) by In-Vitro
title_full Effect of IBA and BAP on Shoot Growth of Tawangmangu Tangerine (Citrus reticulate) by In-Vitro
title_fullStr Effect of IBA and BAP on Shoot Growth of Tawangmangu Tangerine (Citrus reticulate) by In-Vitro
title_full_unstemmed Effect of IBA and BAP on Shoot Growth of Tawangmangu Tangerine (Citrus reticulate) by In-Vitro
title_short Effect of IBA and BAP on Shoot Growth of Tawangmangu Tangerine (Citrus reticulate) by In-Vitro
title_sort effect of iba and bap on shoot growth of tawangmangu tangerine citrus reticulate by in vitro
topic iba
bap
shoot growth
tawangmangu tangerine
url https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/14977
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AT ermaprihastanti effectofibaandbaponshootgrowthoftawangmangutangerinecitrusreticulatebyinvitro
AT sriwidodoagungsuedy effectofibaandbaponshootgrowthoftawangmangutangerinecitrusreticulatebyinvitro