Assessing the Biocompatibility of Tannic Acid-Based Biomaterials: Addressing Challenges in Standard Cytotoxic Assays
In this comprehensive study, we delve into the intricate binding properties of tannic acid (TA) and examine their dual role in the realm of biomaterial development. While TA’s properties can enhance the functionality and performance of biomaterials, they also raise concerns regarding potential biase...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
|
| Series: | Bioengineering |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-5354/12/6/660 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850156286475239424 |
|---|---|
| author | Silvia Cometta Dietmar Werner Hutmacher |
| author_facet | Silvia Cometta Dietmar Werner Hutmacher |
| author_sort | Silvia Cometta |
| collection | DOAJ |
| description | In this comprehensive study, we delve into the intricate binding properties of tannic acid (TA) and examine their dual role in the realm of biomaterial development. While TA’s properties can enhance the functionality and performance of biomaterials, they also raise concerns regarding potential biases in in vitro biocompatibility assessments. We focus on the relevance and constraints of several widely employed cell viability assays, namely the DNA-based PicoGreen assay, the PrestoBlue assay, and the Live/Dead staining technique utilizing fluorescein diacetate (FDA) and propidium iodide (PI). We investigate how these assays perform when applied to TA-coated scaffolds and cell sheets. Through a detailed presentation of our experimental findings, we juxtapose them through a critical review of the existing literature, allowing us to identify and elucidate the limitations these assays face when assessing TA-based biomaterials. In doing so, we aim not only to enhance the understanding of these potential assay biases but also to provide actionable recommendations for accurately evaluating the biocompatibility of TA-modified substances. This dual approach, combining empirical research with literature analysis, offers vital insights for the research community, ensuring that the assessment of TA-coated biomaterials is scientifically sound and reproducible. |
| format | Article |
| id | doaj-art-8a5943f2b4a046939384f4bb02f640dc |
| institution | OA Journals |
| issn | 2306-5354 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj-art-8a5943f2b4a046939384f4bb02f640dc2025-08-20T02:24:35ZengMDPI AGBioengineering2306-53542025-06-0112666010.3390/bioengineering12060660Assessing the Biocompatibility of Tannic Acid-Based Biomaterials: Addressing Challenges in Standard Cytotoxic AssaysSilvia Cometta0Dietmar Werner Hutmacher1Faculty of Engineering, School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD 4000, AustraliaFaculty of Engineering, School of Mechanical, Medical and Process Engineering, Queensland University of Technology, Brisbane, QLD 4000, AustraliaIn this comprehensive study, we delve into the intricate binding properties of tannic acid (TA) and examine their dual role in the realm of biomaterial development. While TA’s properties can enhance the functionality and performance of biomaterials, they also raise concerns regarding potential biases in in vitro biocompatibility assessments. We focus on the relevance and constraints of several widely employed cell viability assays, namely the DNA-based PicoGreen assay, the PrestoBlue assay, and the Live/Dead staining technique utilizing fluorescein diacetate (FDA) and propidium iodide (PI). We investigate how these assays perform when applied to TA-coated scaffolds and cell sheets. Through a detailed presentation of our experimental findings, we juxtapose them through a critical review of the existing literature, allowing us to identify and elucidate the limitations these assays face when assessing TA-based biomaterials. In doing so, we aim not only to enhance the understanding of these potential assay biases but also to provide actionable recommendations for accurately evaluating the biocompatibility of TA-modified substances. This dual approach, combining empirical research with literature analysis, offers vital insights for the research community, ensuring that the assessment of TA-coated biomaterials is scientifically sound and reproducible.https://www.mdpi.com/2306-5354/12/6/660tannic acidbiocompatibilitycytotoxicityDNAPrestoBlue |
| spellingShingle | Silvia Cometta Dietmar Werner Hutmacher Assessing the Biocompatibility of Tannic Acid-Based Biomaterials: Addressing Challenges in Standard Cytotoxic Assays Bioengineering tannic acid biocompatibility cytotoxicity DNA PrestoBlue |
| title | Assessing the Biocompatibility of Tannic Acid-Based Biomaterials: Addressing Challenges in Standard Cytotoxic Assays |
| title_full | Assessing the Biocompatibility of Tannic Acid-Based Biomaterials: Addressing Challenges in Standard Cytotoxic Assays |
| title_fullStr | Assessing the Biocompatibility of Tannic Acid-Based Biomaterials: Addressing Challenges in Standard Cytotoxic Assays |
| title_full_unstemmed | Assessing the Biocompatibility of Tannic Acid-Based Biomaterials: Addressing Challenges in Standard Cytotoxic Assays |
| title_short | Assessing the Biocompatibility of Tannic Acid-Based Biomaterials: Addressing Challenges in Standard Cytotoxic Assays |
| title_sort | assessing the biocompatibility of tannic acid based biomaterials addressing challenges in standard cytotoxic assays |
| topic | tannic acid biocompatibility cytotoxicity DNA PrestoBlue |
| url | https://www.mdpi.com/2306-5354/12/6/660 |
| work_keys_str_mv | AT silviacometta assessingthebiocompatibilityoftannicacidbasedbiomaterialsaddressingchallengesinstandardcytotoxicassays AT dietmarwernerhutmacher assessingthebiocompatibilityoftannicacidbasedbiomaterialsaddressingchallengesinstandardcytotoxicassays |