Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius)
Introduction: Little information is currently available regarding the presence of Anaplasma species in North African dromedaries. To fill this gap in knowledge, the prevalence, risk factors, and genetic diversity of Anaplasma species were investigated in Tunisian dromedary camels. Methodology: A to...
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The Journal of Infection in Developing Countries
2015-10-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/6950 |
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| author | Hanène Belkahia Mourad Ben Said Lotfi Sayahi Alberto Alberti Lilia Messadi |
| author_facet | Hanène Belkahia Mourad Ben Said Lotfi Sayahi Alberto Alberti Lilia Messadi |
| author_sort | Hanène Belkahia |
| collection | DOAJ |
| description | Introduction: Little information is currently available regarding the presence of Anaplasma species in North African dromedaries. To fill this gap in knowledge, the prevalence, risk factors, and genetic diversity of Anaplasma species were investigated in Tunisian dromedary camels.
Methodology: A total of 226 camels from three different bioclimatic areas were sampled and tested for the presence of Anaplasma species by quantitative polymerase chain reaction (qPCR) and nested polymerase chain reaction (nPCR) assays. Detected Anaplasma strains were characterized by 16S rRNA sequence analysis.
Results: Overall infection rate of Anaplasma spp. was 17.7%, and was significantly higher in females. Notably, A. marginale, A. centrale, A. bovis, and A. phagocytophilum were not detected. Animals were severely infested by three tick species belonging to the genus Hyalomma (H. dromedarii, H. impeltatum, and H. excavatum). Alignment, similarity comparison, and phylogenetic analysis of the 16S rRNA sequence variants obtained in this study suggest that Tunisian dromedaries are infected by more than one novel Anaplasma strain genetically related to A. platys.
Conclusions: This study reports the presence of novel Anaplasma sp. strains genetically related to A. platys in dromedaries from various bioclimatic areas of Tunisia. Findings raise new concerns about the specificity of the direct and indirect diagnostic tests routinely used to detect different Anaplasma species in ruminants and provide useful molecular information to elucidate the evolutionary history of bacterial species related to A. platys.
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| format | Article |
| id | doaj-art-8a571a3771b54615a11dcf9f936af2de |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2015-10-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-8a571a3771b54615a11dcf9f936af2de2025-08-20T02:27:18ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802015-10-0191010.3855/jidc.6950Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius)Hanène Belkahia0Mourad Ben Said1Lotfi Sayahi2Alberto Alberti3Lilia Messadi4Ecole Nationale de Médecine Vétérinaire, Institution de la Recherche et de l’Enseignement Supérieur Agricoles, Université de La Manouba, 2020 Sidi Thabet, TunisiaEcole Nationale de Médecine Vétérinaire, Institution de la Recherche et de l’Enseignement Supérieur Agricoles, Université de La Manouba, 2020 Sidi Thabet, TunisiaCommissariat régional au développement agricole de Sidi Bouzid, 9100 Sidi Bouzid, TunisiaUniversità degli Studi di Sassari, Sassari, ItalyUniversità degli Studi di Sassari, Sassari, ItalyIntroduction: Little information is currently available regarding the presence of Anaplasma species in North African dromedaries. To fill this gap in knowledge, the prevalence, risk factors, and genetic diversity of Anaplasma species were investigated in Tunisian dromedary camels. Methodology: A total of 226 camels from three different bioclimatic areas were sampled and tested for the presence of Anaplasma species by quantitative polymerase chain reaction (qPCR) and nested polymerase chain reaction (nPCR) assays. Detected Anaplasma strains were characterized by 16S rRNA sequence analysis. Results: Overall infection rate of Anaplasma spp. was 17.7%, and was significantly higher in females. Notably, A. marginale, A. centrale, A. bovis, and A. phagocytophilum were not detected. Animals were severely infested by three tick species belonging to the genus Hyalomma (H. dromedarii, H. impeltatum, and H. excavatum). Alignment, similarity comparison, and phylogenetic analysis of the 16S rRNA sequence variants obtained in this study suggest that Tunisian dromedaries are infected by more than one novel Anaplasma strain genetically related to A. platys. Conclusions: This study reports the presence of novel Anaplasma sp. strains genetically related to A. platys in dromedaries from various bioclimatic areas of Tunisia. Findings raise new concerns about the specificity of the direct and indirect diagnostic tests routinely used to detect different Anaplasma species in ruminants and provide useful molecular information to elucidate the evolutionary history of bacterial species related to A. platys. https://jidc.org/index.php/journal/article/view/6950Anaplasma speciesDromedary (Camelus dromedarius)Molecular identification16S rRNA geneTunisia |
| spellingShingle | Hanène Belkahia Mourad Ben Said Lotfi Sayahi Alberto Alberti Lilia Messadi Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius) Journal of Infection in Developing Countries Anaplasma species Dromedary (Camelus dromedarius) Molecular identification 16S rRNA gene Tunisia |
| title | Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius) |
| title_full | Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius) |
| title_fullStr | Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius) |
| title_full_unstemmed | Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius) |
| title_short | Detection of novel strains genetically related to Anaplasma platys in Tunisian one-humped camels (Camelus dromedarius) |
| title_sort | detection of novel strains genetically related to anaplasma platys in tunisian one humped camels camelus dromedarius |
| topic | Anaplasma species Dromedary (Camelus dromedarius) Molecular identification 16S rRNA gene Tunisia |
| url | https://jidc.org/index.php/journal/article/view/6950 |
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